FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7223358
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT74922510.372253458848364No Hit
CCCCCCACTGCTAAATTTGACTG5090327.0470271582828925No Hit
CAACGGAATCCCAAAAGCAGCTG4137105.727391609276461No Hit
TTCAAGTAATCCAGGATAGGCT2153552.981369606767379No Hit
CCCCCACTGCTAAATTTGACTG1801392.493840122557957No Hit
AAGCTGCCAGTTGAAGAACTGT1775932.4585933578260972No Hit
TAGCAGCACGTAAATATTGGCG1668392.3097152321676426No Hit
AACATTCAACGCTGTCGGTGAGT1494822.069425328219922No Hit
AACATTCAACGCTGTCGGTGAG1016671.4074755813016604No Hit
TGAGGTAGTAGGTTGTATAGTT970191.343128777502098No Hit
TAGCTTATCAGACTGATGTTGA912301.2629859962637875No Hit
TAGCTTATCAGACTGATGTTGAC893031.2363086531222736No Hit
TAGCTTATCAGACTGATGTTGACT829751.1487039684313032No Hit
TATTGCACTTGTCCCGGCCTGTAA669420.9267434896622873No Hit
TATTGCACTTGTCCCGGCCTGTAT640380.8865405812642817No Hit
TGAGGTAGTAGATTGTATAGTT613510.84934181581475No Hit
TATTGCACTTGTCCCGGCCTGT577860.799988038804113No Hit
AACCGGGCGGAAACACCA569690.7886775098229937No Hit
TTCAAGTAATTCAGGATAGGTT508670.7042015638709863No Hit
CATAAAGTAGAAAGCACTACT495280.6856644790414652No Hit
TGTAAACATCCTTGACTGGAAGCT403720.5589090281832909No Hit
ACCGGGTGCTGTAGGCTT377690.5228731567783294No Hit
TATTGCACTTGTCCCGGCCTGTA329970.45680969986535347No Hit
TTCTCACTACTGCACTTGACTAG326320.4517566483621606No Hit
ACCGGGTGCTGTAGGCTTT306090.42375028345542337No Hit
CTCCCACTGCTTCACTTGACTAG294450.4076358945520906No Hit
ACCGGGTGCTGTAGGCTTA282890.3916322574625264No Hit
CGCCTGTCTGAGCGTCGCT278640.385748567356069No Hit
AGCAGCATTGTACAGGGCTATGA278350.3853470920311578No Hit
CAACGGAATCCCAAAAGCAGCTGT271790.3762654433021318No Hit
CAACGGAATCCCAAAAGCAGCT265530.36759911387473804No Hit
TCGTACGACTCTTAGCGG258260.3575345427985156No Hit
CCCATAAAGTAGAAAGCACTAC253310.3506817743215829No Hit
ACTGGACTTGGAGTCAGAAGGC248370.34384284982137114No Hit
CATTGCACTTGTCTCGGTCTGA223310.30914984415835406No Hit
TGAGGTAGTAGTTTGTACAGTT217050.30048351473096035No Hit
TGTAAACATCCCCGACTGGAAGCT214620.29711942838773875No Hit
TCCTGTCTGAGCGTCGCT213730.295887314459563No Hit
CACCCGGCTGTGTGCACATGTGC213650.2957765626457944No Hit
GGAATACCGGGTGCTGTAGGCTTT213210.29516742767006704No Hit
CCCCCACTGCTAAATTTGACTGGCTT193260.2675486941115199No Hit
CCCATAAAGTAGAAAGCACT165550.22918703461741755No Hit
GGAATACCGGGTGCTGTAGGCTT163980.22701353027220855No Hit
CTTCTCACTACTGCACTTGACTAG158620.2195931587497117No Hit
CAACACCAGTCGATGGGCTGT151820.21017925457937986No Hit
TCGTTTCCCGGCCAATGCACCA151800.2101515666259377No Hit
TGAGGTAGTAGTTTGTGCTGTT148950.20620603326043097No Hit
CCCCCCACTGCTAAATTTGACTGGCT148700.20585993384240406No Hit
CTACGCCTGTCTGAGCGTCGCT144610.20019774736348386No Hit
AGCTCGGTCTGAGGCCCCTCAGT135980.18825039545319505No Hit
TAGCTTATCAGACTGATGTTGACA127180.1760676959386479No Hit
AACGGAATCCCAAAAGCAGCTG124740.1726897656187053No Hit
CCCCCCACTGCTAAATTTGACTGG122400.1694502750659735No Hit
GACCCGCGGGCGCTCTCCAGTCCT121950.16882729611352504No Hit
ACCCCCCACTGCTAAATTTGACTG121740.16853657260238245No Hit
GGGAATACCGGGTGCTGTAGGCTTT116690.1615453643582389No Hit
TGTAAACATCCTACACTCTCAGCT113350.15692147613339946No Hit
GGGAATACCGGGTGCTGTAGGCTT112270.15542632664752323No Hit
TTCAAGTAATCCAGGATAGGCTT108190.1497779841453241No Hit
CTCCCACTGCTTCACTTGACTAGCCT107450.14875352986796445No Hit
GCCTGTCTGAGCGTCGCTT107060.1482136147758425No Hit
TTCAAGTAATCCAGGATAGGC106980.14810286296207387No Hit
TTCACAGTGGCTAAGTTCTGC106710.1477290755906048No Hit
TGAGGTAGTAGGTTGTGTGGTT106280.14713378459159854No Hit
TACGCCTGTCTGAGCGTCGCT105810.14648311768570796No Hit
TTCACAGTGGCTAAGTTCCG104330.14443420913098867No Hit
TAGCAGCACGTAAATATTGGC101960.14115318664809356No Hit
TATTGCACTTGTCCCGGCCTGTT99080.13716612135242362No Hit
TGAGGTAGTAGGTTGTATAGT93940.13005031731779043No Hit
AACATTCAACGCTGTCGGTGAGTT91760.12703233039259582No Hit
ATCCCGGACGAGCCCCCA89140.12340520849167382No Hit
CAACGGAATCCCAAAAGCAGCTGA85400.1182275611979913No Hit
CGCGACCTCAGATCAGACGTGG84590.11710619908358412No Hit
TGATGTAGTAGGTTGTATAGTT84240.11662165989834644No Hit
TTCACAGTGGCTAAGTTCCGC83430.11550029778393926No Hit
CGCGACCTCAGATCAGACGTGGCG82350.11400514829806303No Hit
CAAAGAATTCTCCTTTTGGGCT82120.11368673683347828No Hit
CACTAGATTGTGAGCTCCTGGA81330.11259306267251326No Hit
TCCCACTGCTTCACTTGACTAG81300.11255153074235001No Hit
CAAAGTGCTGTTCGTGCAGGT76580.10601717373000202No Hit
GAATACCGGGTGCTGTAGGCTTT76250.10556032249820652No Hit
AATGCACCCGGGCAAGGATTCT76120.10538035080083251No Hit
ACGCCTGTCTGAGCGTCGCT74570.1032345344090657No Hit
AGAATTACTCTGAGACCT73600.10189166866712131No Hit
TTATAAAGCAATGAGACTGATT73030.10110256199401994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)