FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14533118
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTT11563887.956916058893899No Hit
CAACGGAATCCCAAAAGCAGCTG8348275.744307587676643No Hit
GGAATACCGGGTGCTGTAGGCTT8094255.569520594273025No Hit
CCCCCCACTGCTAAATTTGACTG6027924.147712830791025No Hit
TTCAAGTAATCCAGGATAGGCT4818553.31556518016299No Hit
AAGCTGCCAGTTGAAGAACTGT4534863.1203627466590445No Hit
TAGCAGCACGTAAATATTGGCG4445803.0590820221785853No Hit
AACATTCAACGCTGTCGGTGAGT3451082.3746315140357357No Hit
TGAATACCGGGTGCTGTAGGCTTT2832871.9492513581737931No Hit
TAGCTTATCAGACTGATGTTGAC2618381.8016643090629276No Hit
TAGCTTATCAGACTGATGTTGACT2361891.6251777491932562No Hit
AACATTCAACGCTGTCGGTGAG2136651.4701938014953158No Hit
GGAATACCGGGTGCTGTAGGCTTA2052321.41216771239317No Hit
TGAATACCGGGTGCTGTAGGCTT1981811.3636509384978501No Hit
TAGCTTATCAGACTGATGTTGA1936131.332219280129701No Hit
CCCCCACTGCTAAATTTGACTG1813011.2475024285910292No Hit
TATTGCACTTGTCCCGGCCTGTAT1762171.2125202589010837No Hit
TATTGCACTTGTCCCGGCCTGTAA1656821.1400306527477448No Hit
CATAAAGTAGAAAGCACTACT1650051.1353723268468612No Hit
TGAGGTAGTAGGTTGTATAGTT1602581.1027089988535153No Hit
TGAGGTAGTAGATTGTATAGTT1402810.9652505401800219No Hit
TTCAAGTAATTCAGGATAGGTT1340620.9224586217493039No Hit
TATTGCACTTGTCCCGGCCTGT1314650.9045890909301086No Hit
TGTAAACATCCTTGACTGGAAGCT1312640.9032060429152231No Hit
GGAATACCGGGTGCTGTAGGCT1219260.8389527973281439No Hit
TCGTACGACTCTTAGCGG1049530.7221643696830921No Hit
ATCCCGGACGAGCCCCCA989860.6811064218979025No Hit
TATTGCACTTGTCCCGGCCTGTA803470.5528545216518574No Hit
ACTGGACTTGGAGTCAGAAGGC762830.524890804574765No Hit
AGCAGCATTGTACAGGGCTATGA728400.501200086588439No Hit
CATTGCACTTGTCTCGGTCTGA647220.44534146079320347No Hit
TGTAAACATCCCCGACTGGAAGCT613510.4221461629913141No Hit
TGAGGTAGTAGTTTGTACAGTT599870.41276070283059696No Hit
CCCATAAAGTAGAAAGCACTAC556790.38311806179513574No Hit
CCCATAAAGTAGAAAGCACT544470.37464087197255264No Hit
CAACGGAATCCCAAAAGCAGCTGT534160.36754673016485523No Hit
TGAATACCGGGTGCTGTAGGCTTA522840.3597576239317674No Hit
AACCGGGCGGAAACACCA520930.3584433842758312No Hit
TGTAAACATCCTACACTCTCAGCT463390.3188510545362668No Hit
TTCTCACTACTGCACTTGACTAG454230.31254820885648904No Hit
CAACACCAGTCGATGGGCTGT447050.30760776868391215No Hit
CGCCTGTCTGAGCGTCGCT446620.30731189274042914No Hit
CTCCCACTGCTTCACTTGACTAG439210.3022131933422683No Hit
GCCTGTCTGAGCGTCGCT398940.2745040671932891No Hit
CTTCTCACTACTGCACTTGACTAG367450.25283631496007947No Hit
TGAGGTAGTAGTTTGTGCTGTT338420.2328612483570284No Hit
TGTCAGTTTGTCAAATACCCCAA332400.2287189851482662No Hit
CAACGGAATCCCAAAAGCAGCT322070.22161108166877885No Hit
GGAATACCGGGTGCTGTAGGCTA321240.2210399722894977No Hit
ATACCGGGTGCTGTAGGCTTA317320.2183426846186758No Hit
TTATAAAGCAATGAGACTGATT303240.20865446767858076No Hit
TGAATACCGGGTGCTGTAGGCT297920.20499386298246527No Hit
CAAAGAATTCTCCTTTTGGGCT278440.19158999465909518No Hit
CACTAGATTGTGAGCTCCTGGA274420.18882389862932375No Hit
TGTCAGTTTGTCAAATACCCCA265290.18254169545723087No Hit
TTCACAGTGGCTAAGTTCCG263330.1811930516218199No Hit
AACATTCAACGCTGTCGGTGAGTT258100.17759437444875903No Hit
ATACCGGGTGCTGTAGGCTTT257530.17720216680274667No Hit
AGCTCGGTCTGAGGCCCCTCAGT252860.1739888164398032No Hit
CTACGCCTGTCTGAGCGTCGCT251940.17335577953746747No Hit
TAGCAGCACGTAAATATTGGC249020.17134657545614093No Hit
TTCACAGTGGCTAAGTTCCGC248610.17106446118444782No Hit
TTCAAGTAATCCAGGATAGGCTT245890.16919287382102038No Hit
TATTGCACTTGTCCCGGCCTGTT234420.1613005550495083No Hit
TTCAAGTAATCCAGGATAGGC234320.16123174669055876No Hit
AACGGAATCCCAAAAGCAGCTG232520.1599931962294671No Hit
TCGTTTCCCGGCCAATGCACCA231790.15949089520913545No Hit
CAAAGAATTCTCCTTTTGGGCTT227420.1564839699230406No Hit
CACTAGATTGTGAGCTCCTGGAA217560.1496994657306161No Hit
ATACCGGGTGCTGTAGGCTT217210.1494586364742927No Hit
TAGCTTATCAGACTGATGTTGACA207350.14267413228186823No Hit
TCGTTTCCCGGGCGGCGCACCA205910.14168329191299486No Hit
TGAGAACTGAATTCCATGGGTT205630.14149062850793614No Hit
TGTAAACATCCTACACTCAGCT199770.1374584586734932No Hit
TATTGCACTTGTCCCGGCCTGTAG198600.13665340087378358No Hit
ACTGGACTTGGAGTCAGAAGGCT196040.13489190688467542No Hit
CGAATACCGGGTGCTGTAGGCTTT191150.13152717813204295No Hit
TGTAAACATCCCCGACTGGAAGC190570.13112808965013564No Hit
TACGCCTGTCTGAGCGTCGCT189620.13047441024011502No Hit
TTCACAGTGGCTAAGTTCTGC187970.12933907231744765No Hit
AGGTCCCTGTTCGGGCGCCA187140.12876796293816647No Hit
CACCCGGCTGTGTGCACATGTGC182310.12544451920090377No Hit
ACTGGACTTGGAGTCAGAAGGCA177450.12210043295595618No Hit
ACTGGACTTGGAGTCAGAAGG173510.11938938361334436No Hit
GAATACCGGGTGCTGTAGGCTTT172310.11856368330594991No Hit
CCCCCCACTGCTAAATTTGACTGGCT171740.11817147565993753No Hit
CCCCCACTGCTAAATTTGACTGGCTT167780.11544666464553581No Hit
CCCATAAAGTAGAAAGCACTA167520.115267762912267No Hit
ACCGGGTGCTGTAGGCTTA160200.11023099103716079No Hit
TATTGCACTTGTCCCGGCCTGTTT158620.10914381896575807No Hit
AGCAGCATTGTACAGGGCTATGT156070.10738920581254484No Hit
TGAGGTAGTAGGTTGTATAGT151650.10434787634697523No Hit
CTCCTGGCTGGCTCGCCA150340.10344648684473627No Hit
TTCACAGTGGCTAAGTTCTG150300.10341896350115647No Hit
TGTCAGTTTGTCAAATACCCC148410.1021184855170102No Hit
ATCCCACCGCTGCCACCA147910.10177444372226249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)