FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5204957
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG2527774.856466633634053No Hit
TTCAAGTAATCCAGGATAGGCT2270114.361438528694857No Hit
AAGCTGCCAGTTGAAGAACTGT2266464.354425982769886No Hit
TAGCAGCACGTAAATATTGGCG1936713.7208952926988634No Hit
CCCCCCACTGCTAAATTTGACTG1532502.94430866575843No Hit
AACATTCAACGCTGTCGGTGAGT1389402.6693784405903833No Hit
TAGCTTATCAGACTGATGTTGAC1151522.2123525708281546No Hit
TGAGGTAGTAGGTTGTATAGTT1044242.0062413579977703No Hit
TAGCTTATCAGACTGATGTTGA1020311.960265954166384No Hit
TGAGGTAGTAGATTGTATAGTT875561.6821656739911588No Hit
AACATTCAACGCTGTCGGTGAG742681.4268705774130315No Hit
TTCAAGTAATTCAGGATAGGTT735171.412442024016721No Hit
TAGCTTATCAGACTGATGTTGACT689071.3238726083616061No Hit
CGCCTGTCTGAGCGTCGCT662531.272882753882501No Hit
CATAAAGTAGAAAGCACTACT640291.2301542548766493No Hit
CCCCCACTGCTAAATTTGACTG610761.1734198764754444No Hit
TATTGCACTTGTCCCGGCCTGT566521.0884239773738764No Hit
TGTAAACATCCTTGACTGGAAGCT529981.0182216683058092No Hit
TCGTACGACTCTTAGCGG404330.7768171763955014No Hit
TATTGCACTTGTCCCGGCCTGTAT377920.7260770838260527No Hit
GCCTGTCTGAGCGTCGCT361950.6953947938474804No Hit
TATTGCACTTGTCCCGGCCTGTAA355170.6823687496361641No Hit
TGAGGTAGTAGTTTGTACAGTT345750.6642706174133619No Hit
AGCAGCATTGTACAGGGCTATGA296570.5697837657448467No Hit
TTCTCACTACTGCACTTGACTAG290640.5583907801735922No Hit
CATTGCACTTGTCTCGGTCTGA285450.5484195162419209No Hit
ACTGGACTTGGAGTCAGAAGGC259610.498774533584043No Hit
AACCGGGCGGAAACACCA257260.49425960675563696No Hit
TATTGCACTTGTCCCGGCCTGTA250810.48186757354575654No Hit
TGAGGTAGTAGTTTGTGCTGTT226350.43487390962115535No Hit
TGTAAACATCCCCGACTGGAAGCT197440.3793307034044662No Hit
TAGTGGGTTATCAGAACT192980.3707619486577891No Hit
TGTAAACATCCTACACTCTCAGCT189440.36396073973329657No Hit
TGTCAGTTTGTCAAATACCCCAA185180.35577623407839876No Hit
CCCATAAAGTAGAAAGCACT180990.34772621560562367No Hit
TTATAAAGCAATGAGACTGATT178780.34348026314146307No Hit
CTACGCCTGTCTGAGCGTCGCT176930.33992595904250505No Hit
TACGCCTGTCTGAGCGTCGCT176280.33867714949422256No Hit
ACGCCTGTCTGAGCGTCGCT169040.32476733237181404No Hit
CCCATAAAGTAGAAAGCACTAC168470.3236722224602432No Hit
CAACGGAATCCCAAAAGCAGCTGT164150.3153724420778116No Hit
ATCCCGGACGAGCCCCCA162130.31149152625084126No Hit
TTCACAGTGGCTAAGTTCCG160860.3090515445180431No Hit
CTCCCACTGCTTCACTTGACTAG158150.3038449693244344No Hit
CAACACCAGTCGATGGGCTGT157840.30324938323217654No Hit
TGTCAGTTTGTCAAATACCCCA150430.28901295438175567No Hit
CTTCTCACTACTGCACTTGACTAG144310.27725493217331093No Hit
TTAATGCTAATCGTGATAGGGGTT141900.2726247306173711No Hit
TGATGTAGTAGGTTGTATAGTT141330.2715296207058003No Hit
TCCTGTCTGAGCGTCGCT136340.26194260586590823No Hit
TACCTGGTTGATCCTGCC125620.24134685454654092No Hit
TGTAAACATCCTACACTCAGCT124810.239790645724835No Hit
TTCACAGTGGCTAAGTTCCGC122020.2344303708945146No Hit
TTCAAGTAATCCAGGATAGGC119660.22989623161151956No Hit
TGAGGTAGTAGGTTGTATAGT118990.22860899715405908No Hit
TGATGTAGTAGATTGTATAGTT116340.22351769668798416No Hit
TAGCAGCACGTAAATATTGGC113120.2173312863103384No Hit
ACCGGGTGCTGTAGGCTT111450.21412280639398173No Hit
AACATTCAACGCTGTCGGTGAGTT110680.21264344739063165No Hit
TGAGGTAGTAGATTGTATAGT105960.20357516882464158No Hit
TTCAAGTAATCCAGGATAGGCTT105220.2021534471850584No Hit
TAGCAGCACGTAAATATTGGCGT104100.20000165227109465No Hit
TTCACAGTGGCTAAGTTCTGC100980.1940073664393385No Hit
TAGCAGCACATAATGGTTTGT100720.1935078426200255No Hit
ATCCCACCGCTGCCACCA96090.18461247614533607No Hit
GCGTTCGCGCTTTCCCCTG93520.17967487531597284No Hit
ACCGGGTGCTGTAGGCTTT82940.159348098360851No Hit
TAGCTTATCAGACTGATGTTGACA82370.15825298844928018No Hit
CAAAGTGCTGTTCGTGCAGGT78680.1511635927059532No Hit
TATTGCACTTGTCCCGGCCTGTT77150.148224087153842No Hit
TTCACAGTGGCTAAGTTCTG74820.14374758523461384No Hit
CAAAGAATTCTCCTTTTGGGCT74530.14319042405153395No Hit
TGAGGTAGTAGGTTGTGTGGTT74250.142652475323043No Hit
AGCTCGGTCTGAGGCCCCTCAGT74230.1426140504138651No Hit
TGAGGTAGTAAGTTGTATTGTT73990.14215295150373No Hit
AGAGGTAGTAGGTTGCATAGTT73830.14184555223030662No Hit
AACGGAATCCCAAAAGCAGCTG72610.13950163277045324No Hit
CAAAGAATTCTCCTTTTGGGCTT71260.1369079514009434No Hit
ACTGGACTTGGAGTCAGAAGG71210.13681188912799855No Hit
GGAATACCGGGTGCTGTAGGCTTT70850.13612024076279594No Hit
TGTAAACATCCCCGACTGGAAGC70470.13539016748841537No Hit
AGCAGCATTGTACAGGGCTATG67750.1301643798402177No Hit
TAGCTTATCAGACTGATGTTG67700.13006831756727288No Hit
CCCCCACTGCTAAATTTGACTGGCTT65480.12580315264852332No Hit
CAACGGAATCCCAAAAGCAGCT65010.1249001672828421No Hit
TGTCAGTTTGTCAAATACCCC64770.12443906837270702No Hit
AACATTCAACGCTGTCGGTGA64620.12415088155387259No Hit
TCCCACTGCTTCACTTGACTAG63240.12149956282059583No Hit
CTCGACACAAGGGTTTGT63100.12123058845635035No Hit
TCCCCGGCACCTCCACCA62730.12051972763655876No Hit
CCTCGACACAAGGGTTTGT62020.11915564336074246No Hit
GGAATACCGGGTGCTGTAGGCTT61940.11900194372403076No Hit
TGAGAACTGAATTCCATGGGTT61100.11738809753855796No Hit
ACCACAGGGTAGAACCACGGAC61010.1172151854472573No Hit
CCCCCCACTGCTAAATTTGACTGGCT60970.11713833562890145No Hit
AGCAGCATTGTACAGGGCTATGT58360.11212388498118236No Hit
CCCCCCACTGCTAAATTTGACTGG57840.11112483734255632No Hit
CTCCCACTGCTTCACTTGACTAGCCT57510.11049082634112059No Hit
TCGTTTCCCGGCCAATGCACCA56930.10937650397496079No Hit
ACCGGGTGCTGTAGGCTTA55500.10662912296873922No Hit
AGAATTACTCTGAGACCT55000.10566850023929113No Hit
CAAAGTGCTGTTCGTGCAGGTAG54610.10491921451032162No Hit
ATCACATTGCCAGGGATTTCC53580.10294033168765852No Hit
CACTAGATTGTGAGCTCCTGGA53440.10267135732341304No Hit
TTCACCACCTTCTCCACCCAGC53170.10215262104951107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)