FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8072142
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5589496.924419813229251No Hit
CCCCCCACTGCTAAATTTGACTG3593764.452052503536237No Hit
TTCAAGTAATCCAGGATAGGCT3113683.8573156914236644No Hit
AAGCTGCCAGTTGAAGAACTGT2990853.705150380159318No Hit
AACATTCAACGCTGTCGGTGAGT2968823.6778589871189085No Hit
TAGCAGCACGTAAATATTGGCG2747233.4033469678803967No Hit
TAGCTTATCAGACTGATGTTGAC1688942.0923070976699867No Hit
AACATTCAACGCTGTCGGTGAG1608251.992346021663147No Hit
CCCCCACTGCTAAATTTGACTG1409641.7463022825911636No Hit
TAGCTTATCAGACTGATGTTGACT1295241.6045802960354267No Hit
TAGCTTATCAGACTGATGTTGA1194981.4803753452305473No Hit
TGAGGTAGTAGGTTGTATAGTT1165981.4444493171700894No Hit
GCCTGTCTGAGCGTCGCT996211.234133393589954No Hit
CGCCTGTCTGAGCGTCGCT922691.1430547183139246No Hit
TATTGCACTTGTCCCGGCCTGT851871.055320880133179No Hit
TATTGCACTTGTCCCGGCCTGTAA841951.0430317008793948No Hit
TGAGGTAGTAGATTGTATAGTT819821.0156164249836042No Hit
TGTAAACATCCTTGACTGGAAGCT798320.9889816110767129No Hit
TATTGCACTTGTCCCGGCCTGTAT787990.9761845121158672No Hit
CATAAAGTAGAAAGCACTACT741960.919161233784044No Hit
TTCAAGTAATTCAGGATAGGTT676310.8378321392264904No Hit
TCGTACGACTCTTAGCGG658440.8156942729699255No Hit
ACTGGACTTGGAGTCAGAAGGC601460.745105821973895No Hit
AGCAGCATTGTACAGGGCTATGA527180.6530856369969705No Hit
CCCATAAAGTAGAAAGCACT478030.5921972135772636No Hit
TATTGCACTTGTCCCGGCCTGTA468020.5797965397536367No Hit
TTAATGCTAATCGTGATAGGGGTT444620.5508079515945086No Hit
CCCATAAAGTAGAAAGCACTAC411000.5091585356154538No Hit
TGTAAACATCCCCGACTGGAAGCT393560.48755336563702667No Hit
AACCGGGCGGAAACACCA393310.4872436584985745No Hit
CATTGCACTTGTCTCGGTCTGA382630.47401296954389555No Hit
TGAGGTAGTAGTTTGTACAGTT376420.4663198442247423No Hit
CAACGGAATCCCAAAAGCAGCTGT343320.4253146190936681No Hit
TGTCAGTTTGTCAAATACCCCAA315450.3907884672990143No Hit
TGTAAACATCCTACACTCTCAGCT309350.3832316131207801No Hit
TCCTGTCTGAGCGTCGCT283520.3512326715758965No Hit
TTCTCACTACTGCACTTGACTAG277900.3442704551034905No Hit
TACGCCTGTCTGAGCGTCGCT252300.3125564441259829No Hit
CAACACCAGTCGATGGGCTGT234840.2909264975764797No Hit
AACATTCAACGCTGTCGGTGAGTT230930.28608267793108694No Hit
TGAGGTAGTAGTTTGTGCTGTT217840.2698664121617286No Hit
TTATAAAGCAATGAGACTGATT207000.2564375106384402No Hit
GGAATACCGGGTGCTGTAGGCTTT205830.2549880812304838No Hit
TGTCAGTTTGTCAAATACCCCA201710.24988410758879118No Hit
ATCCCGGACGAGCCCCCA198250.24559776079261242No Hit
ACGCCTGTCTGAGCGTCGCT197590.24478013394709855No Hit
CTACGCCTGTCTGAGCGTCGCT197090.2441607196701941No Hit
CTTCTCACTACTGCACTTGACTAG185640.22997613272908232No Hit
TTCAAGTAATCCAGGATAGGCTT169120.20951068502015946No Hit
AACGGAATCCCAAAAGCAGCTG168670.20895321217094545No Hit
CTCCCACTGCTTCACTTGACTAG166020.2056703165033519No Hit
TAGCAGCACGTAAATATTGGC161800.20044246000627836No Hit
TAGCTTATCAGACTGATGTTGACA160980.19942662059215507No Hit
GGAATACCGGGTGCTGTAGGCTT156330.19366606781694376No Hit
CAACGGAATCCCAAAAGCAGCT154850.1918326015573066No Hit
TTCAAGTAATCCAGGATAGGC148730.18425097080799618No Hit
TGTAAACATCCTACACTCAGCT148170.1835572268178632No Hit
ATCCCACCGCTGCCACCA142100.17603753749624323No Hit
GCGTTCGCGCTTTCCCCTG142050.17597559606855281No Hit
ACTGGACTTGGAGTCAGAAGGCA135630.16802231675309973No Hit
TTCACAGTGGCTAAGTTCCG131980.1635005925316973No Hit
ACTGGACTTGGAGTCAGAAGG128870.15964783572935162No Hit
TATTGCACTTGTCCCGGCCTGTT128400.15906558630906148No Hit
CCCCCACTGCTAAATTTGACTGGCTT126410.15660031748698178No Hit
CAAAGAATTCTCCTTTTGGGCT123790.15335458667600246No Hit
ACTGGACTTGGAGTCAGAAGGCT123580.1530944326797026No Hit
TGAGGTAGTAGGTTGTGTGGTT122580.15185560412589372No Hit
TAGCAGCACGTAAATATTGGCGT122510.1517688861271271No Hit
ACCGGGTGCTGTAGGCTT120890.1497619838699567No Hit
TGAGGTAGTAGGTTGTATAGT119690.14827538960538603No Hit
ACCACAGGGTAGAACCACGGAC117690.14579773249776823No Hit
CACTAGATTGTGAGCTCCTGGA115420.14298559168062205No Hit
TTCACAGTGGCTAAGTTCCGC114380.14169720998466082No Hit
TAGTGGGTTATCAGAACT111870.13858775031460052No Hit
AACATTCAACGCTGTCGGTGA110450.1368286137681919No Hit
CAAAGAATTCTCCTTTTGGGCTT110370.13672950748388718No Hit
TCGTTTCCCGGCCAATGCACCA109840.13607292835036847No Hit
AGCTCGGTCTGAGGCCCCTCAGT109660.13584993921068286No Hit
TTCACAGTGGCTAAGTTCTGC107570.1332607875332223No Hit
AGCAGCATTGTACAGGGCTATGT107490.13316168124891756No Hit
CCCCCCACTGCTAAATTTGACTGGCT107450.13311212810676523No Hit
TGTAAACATCCCCGACTGGAAGC106810.13231927783232753No Hit
AGCAGCATTGTACAGGGCTATG97260.12048846514345264No Hit
AGAGGTAGTAGGTTGCATAGTT96900.12004248686408144No Hit
TATTGCACTTGTCCCGGCCTGTAG96660.11974516801116729No Hit
TACCTGGTTGATCCTGCC95940.1188532114524249No Hit
CCCATAAAGTAGAAAGCACTA94840.11749050004323512No Hit
CCCCCCACTGCTAAATTTGACTGG94560.11714362804816864No Hit
CACCCGGCTGTGTGCACATGTGC93610.11596674092205017No Hit
CGCGACCTCAGATCAGAC93100.11533493835960765No Hit
ACCGGGTGCTGTAGGCTTT87760.10871959388226816No Hit
CAAAGTGCTGTTCGTGCAGGT86980.10775330761029724No Hit
TTCACAGTGGCTAAGTTCTG86720.10743121218630694No Hit
CACTAGATTGTGAGCTCCTGGAA86500.10715866990446898No Hit
ATCACATTGCCAGGGATTTCC86330.10694806905032145No Hit
TGTCAGTTTGTCAAATACCCC84360.10450757679931796No Hit
TGAGGTAGTAGATTGTATAGT83600.1035660670984232No Hit
TAGCAGCACATAATGGTTTGT81150.1005309371415914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)