FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7688934
Sequences flagged as poor quality0
Sequence length18-26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG119284815.513828054708235No Hit
CCCCCCACTGCTAAATTTGACTG3859045.018953212499938No Hit
TTCAAGTAATCCAGGATAGGCT3489424.538236379711414No Hit
TAGCAGCACGTAAATATTGGCG2871723.7348740410569263No Hit
CCCCCACTGCTAAATTTGACTG1873432.4365276122801935No Hit
TCGTACGACTCTTAGCGG1856302.414248841256798No Hit
AAGCTGCCAGTTGAAGAACTGT1806602.3496104921696555No Hit
TAGCTTATCAGACTGATGTTGAC1804992.347516573818946No Hit
AACATTCAACGCTGTCGGTGAG1599622.080418429914992No Hit
TGAGGTAGTAGATTGTATAGTT1305081.6973484230713907No Hit
TAGCTTATCAGACTGATGTTGA1241441.614580122550148No Hit
TGAGGTAGTAGGTTGTATAGTT967901.2588220941940718No Hit
TAGCTTATCAGACTGATGTTGACT914271.1890725034185492No Hit
TTAATGCTAATCGTGATAGGGGTT866151.126489055569992No Hit
TGAGAACTGAATTCCATGGGTT676190.8794327015942652No Hit
TGTAAACATCCTTGACTGGAAGCT666940.8674024253557125No Hit
TATTGCACTTGTCCCGGCCTGT646690.8410658746713133No Hit
TTCAAGTAATTCAGGATAGGTT614670.7994216103298585No Hit
TTCTCACTACTGCACTTGACTAG550330.7157429105256984No Hit
CAACGGAATCCCAAAAGCAGCTGT545570.7095521954018593No Hit
AACATTCAACGCTGTCGGTGAGT493530.6418705115689639No Hit
CATTGCACTTGTCTCGGTCTGA471150.6127637459236872No Hit
TGAGGTAGTAGTTTGTACAGTT455460.5923577962822936No Hit
AACCGGGCGGAAACACCA438110.5697928997699812No Hit
CATAAAGTAGAAAGCACTACT426430.5546022374493006No Hit
TGAGAACTGAATTCCATAGGCTGT425180.5529765244440906No Hit
TATTGCACTTGTCCCGGCCTGTAA419930.5461485298222094No Hit
TATTGCACTTGTCCCGGCCTGTAT399800.5199680475863104No Hit
AGCAGCATTGTACAGGGCTATGA393670.5119955510087615No Hit
CAACGGAATCCCAAAAGCAGCT378430.4921748580492432No Hit
TATTGCACTTGTCCCGGCCTGTA376530.48970377428132433No Hit
CCCATAAAGTAGAAAGCACT370340.48165324347952526No Hit
TGATGTAGTAGATTGTATAGTT332170.432010471152438No Hit
CCCATAAAGTAGAAAGCACTAC305000.3966739732711973No Hit
TGTAAACATCCCCGACTGGAAGCT273280.3554198800509928No Hit
CTTCTCACTACTGCACTTGACTAG271000.35245457952949005No Hit
TAGCAGCACGTAAATATTGGC267350.3477074975542773No Hit
GGAATACCGGGTGCTGTAGGCTTT260990.3394358697837697No Hit
AACGGAATCCCAAAAGCAGCTG260530.3388376073978525No Hit
ACTGGACTTGGAGTCAGAAGGC257070.33433763379943177No Hit
TGATGTAGTAGGTTGTATAGTT247720.3221773005204623No Hit
CCCCCACTGCTAAATTTGACTGGCTT237480.3088594595817834No Hit
CTCCCACTGCTTCACTTGACTAG221060.2875040935453471No Hit
GCCTGTCTGAGCGTCGCT215580.28037696773050724No Hit
GGAATACCGGGTGCTGTAGGCTT209320.27223539700041643No Hit
TAGCTTATCAGACTGATGTTGACA208930.27172817454279097No Hit
TGTCAGTTTGTCAAATACCCCA208350.27097384370837363No Hit
TGTAAACATCCTACACTCTCAGCT201470.26202591932769875No Hit
CAAAGAATTCTCCTTTTGGGCT195800.254651685136067No Hit
TCGTTTCCCGGCCAATGCACCA195150.2538063143733579No Hit
TTCACAGTGGCTAAGTTCCGC168290.21887299331740914No Hit
CCCATAAAGTAGAAAGCACTA165280.2149582764008639No Hit
TGATAACTGAATTCCATGGGTT154720.2012242529328513No Hit
TTCACAGTGGCTAAGTTCTGC148680.1933688076916774No Hit
CAACACCAGTCGATGGGCTGT148290.19286158523405195No Hit
TTCAAGTAATCCAGGATAGGC145150.18877779416496487No Hit
CACCCGTAGAACCGACCTTGCG142250.18500613999287807No Hit
TGAGGTAGTAGATTGTATAGT141900.1845509403514193No Hit
AGCTCGGTCTGAGGCCCCTCAGT134270.17462758816761853No Hit
TGAGGTAGTAGTTTGTGCTGTT127330.16560162956269361No Hit
ATCACATTGCCAGGGATTTCC125550.1632866142432748No Hit
AGCTACATTGTCTGCTGGGTTT124200.1615308441976482No Hit
CTCCCACTGCTTCACTTGACTAGCCT123240.16028229660964707No Hit
TTAATGCTAATCGTGATAGGGGT119600.15554822033847604No Hit
TTCACAGTGGCTAAGTTCTG119520.1554441747061426No Hit
TTAATGCTAATCGTGATAGGGGTTT118390.15397453014943294No Hit
TTCACAGTGGCTAAGTTCCG116190.15111327526026366No Hit
TGGCTCAGTTCAGCAGGAAC115620.15037195012988797No Hit
TGATGTAGTAGTTTGTACAGTT112010.145676890970842No Hit
CACTAGATTGTGAGCTCCTGGA111940.14558585104255023No Hit
TGAGGTAGTAAGTTGTATTGTT106360.13832866818729359No Hit
TGTAAACATCCCCGACTGGA105310.13696306926291735No Hit
TTCAAGTAATCCAGGATAGGCTT105280.13692405215079229No Hit
TATTGCACTTGTCCCGGCCTGTT103270.1343099056384149No Hit
CCCCCCACTGCTAAATTTGACTGG101110.1315006735654123No Hit
TGATAACTGAATTCCATAGGCTGT99020.12878248142070148No Hit
CAAAGAATTCTCCTTTTGGGCTT95930.12476371887182282No Hit
CCCCCCACTGCTAAATTTGACTGGCT95680.12443857627078084No Hit
TTATAAAGCAATGAGACTGATT93470.12156431567756987No Hit
CAAAGTGCTGTTCGTGCAGGTAG93100.12108310462802777No Hit
CACCCGGCTGTGTGCACATGTGC90840.11814381551460841No Hit
TCCCACTGCTTCACTTGACTAG90040.11710335919127413No Hit
TGAGAACTGAATTCCATGGGTTG89120.11590683441943968No Hit
ACTGGACTTGGAGTCAGAAGGCT88210.11472331535164691No Hit
TGAATACCGGGTGCTGTAGGCTTT85640.11138084941293552No Hit
TAGCAGCACATCATGGTTTACA85600.1113288265967688No Hit
CACTAGATTGTGAGCTCCTGGAA84860.11036640449768459No Hit
CAAAGAATTCTCCTTTTGGGC81070.1054372426658884No Hit
TGTCAGTTTGTCAAATACCCCAA80840.1051381114729298No Hit
TCCTGTACTGAGCTGCCCCGAGA78840.10253697066459407No Hit
AATGCACCCGGGCAAGGATTCT77900.10131443448467628No Hit
ACCCCCCACTGCTAAATTTGACTG76950.10007889260071681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)