FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13717244
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8508216.2025651800026305No Hit
CCCCCCACTGCTAAATTTGACTG6545984.772081039019208No Hit
TAGCAGCACGTAAATATTGGCG5283313.8515827231767545No Hit
AAGCTGCCAGTTGAAGAACTGT4588203.344840989924798No Hit
TTCAAGTAATCCAGGATAGGCT4502073.2820514091606157No Hit
AACATTCAACGCTGTCGGTGAGT4241063.0917726622053237No Hit
TAGCTTATCAGACTGATGTTGAC2944982.146918141865815No Hit
AACATTCAACGCTGTCGGTGAG2741881.998856330032476No Hit
CCCCCACTGCTAAATTTGACTG1918361.3985024980236556No Hit
TAGCTTATCAGACTGATGTTGACT1917121.3975985263512116No Hit
TCCACCACGTTCCCGTGG1823161.329100801881194No Hit
TATTGCACTTGTCCCGGCCTGTAA1771881.2917171991691627No Hit
TGAGGTAGTAGGTTGTATAGTT1514911.1043836502434454No Hit
TGTAAACATCCTTGACTGGAAGCT1435791.0467044254662234No Hit
TATTGCACTTGTCCCGGCCTGTAT1416151.0323866805897743No Hit
CGCCTGTCTGAGCGTCGCT1319220.9617237981623714No Hit
TAGCTTATCAGACTGATGTTGA1297310.9457512019178197No Hit
AGCAGCATTGTACAGGGCTATGA1052400.7672095065160319No Hit
CATAAAGTAGAAAGCACTACT1019970.7435677312439729No Hit
ACTGGACTTGGAGTCAGAAGGC969990.7071318407691808No Hit
GTCAGGATGGCCGAGCGGTCTAAGG960630.7003083126610564No Hit
TAGCAGCACATCATGGTTTACA947960.6910717633950377No Hit
TGAGGTAGTAGATTGTATAGTT889610.6485340641312497No Hit
TATTGCACTTGTCCCGGCCTGT883810.644305809534335No Hit
GCCTGTCTGAGCGTCGCT846670.6172303999258161No Hit
TATTGCACTTGTCCCGGCCTGTA759450.5536461989011787No Hit
CTACGCCTGTCTGAGCGTCGCT747940.5452553005545429No Hit
TCGTTTCCCGGCCAATGCACCA716350.5222258931896232No Hit
CCCCCACTGCTAAATTTGACTGGCTT708240.5163136268480752No Hit
TTCAAGTAATTCAGGATAGGTT697210.5082726530198048No Hit
TACGCCTGTCTGAGCGTCGCT691360.5040079479522271No Hit
TGTAAACATCCCCGACTGGAAGCT677310.493765365695908No Hit
TGTAAACATCCTACACTCTCAGCT588060.42870127556235055No Hit
TTCACAGTGGCTAAGTTCCGC569230.4149740283106431No Hit
TGTCAGTTTGTCAAATACCCCAA487840.35563995216531835No Hit
CAACGGAATCCCAAAAGCAGCTGT479350.3494506622467312No Hit
CCCATAAAGTAGAAAGCACTAC472990.3448141623783903No Hit
AACATTCAACGCTGTCGGTGAGTT442150.3223315120734165No Hit
CATTGCACTTGTCTCGGTCTGA425030.30985087091838565No Hit
AACCGGGCGGAAACACCA419160.30557158566254267No Hit
ACGCCTGTCTGAGCGTCGCT414720.3023347838676632No Hit
TGAGGTAGTAGTTTGTACAGTT394810.2878202064496338No Hit
ATCACATTGCCAGGGATTTCCA389520.2839637466534823No Hit
TTCACAGTGGCTAAGTTCCG389300.28380336458256483No Hit
TTCTCACTACTGCACTTGACTAG373240.2720954734055908No Hit
TAGCAGCACGTAAATATTGGC369200.26915027537601577No Hit
ATCACATTGCCAGGGATTTCC360800.26302659630462216No Hit
CCCCCCACTGCTAAATTTGACTGGCT358360.2612478133362649No Hit
TGTCAGTTTGTCAAATACCCCA357490.2606135751467277No Hit
TAGCTTATCAGACTGATGTTGACA353400.25763192664648965No Hit
TCGTACGACTCTTAGCGG351260.25607184650211073No Hit
TGTAAACATCCTACACTCAGCT339990.24785591041465763No Hit
CAAAGAATTCTCCTTTTGGGCT335360.2444805968312585No Hit
CTTCTCACTACTGCACTTGACTAG329220.2400044790338351No Hit
GCGTTCGCGCTTTCCCCTG324300.23641775272059024No Hit
GTCAGGATGGCCGAGTGGTCTAAGG320710.23380060892698268No Hit
CTCCCACTGCTTCACTTGACTAG311850.22734158552548894No Hit
TCAGGCTCAGTCCCCTCCCGAT304830.22222393944439567No Hit
CCCATAAAGTAGAAAGCACT295840.21567014481917798No Hit
ACTGGACTTGGAGTCAGAAGGCA292400.21316235243755963No Hit
TGAGGTAGTAGTTTGTGCTGTT291870.21277597744853122No Hit
TAGCAGCACGTAAATATTGGCGT290990.2121344491648614No Hit
TTAATGCTAATCGTGATAGGGGTT283230.20647733611795488No Hit
CCAAGTCCTTCTGATCGAGGCCC271770.19812288824198215No Hit
CCCCCCACTGCTAAATTTGACTGG266640.19438306995195245No Hit
ACTGGACTTGGAGTCAGAAGG245220.17876768831989867No Hit
AACGGAATCCCAAAAGCAGCTG232680.16962591027760388No Hit
CAACACCAGTCGATGGGCTGT222480.16219001426234017No Hit
TGAGGTAGTAGGTTGTGTGGTT220280.16058619355316564No Hit
TCGTTTCCCGGCCAACGCACCA218670.1594124883978152No Hit
GGGCTACGCCTGTCTGAGCGTCGCT218650.15939790820954997No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT207960.1516047975817883No Hit
AGCTCGGTCTGAGGCCCCTCAGT205380.14972395329557453No Hit
CAAAGAATTCTCCTTTTGGGCTT203410.14828780475145006No Hit
ACTGGACTTGGAGTCAGAAGGCT199570.14548840860452728No Hit
TTCAAGTAATCCAGGATAGGC196810.1434763426239265No Hit
TTCAAGTAATCCAGGATAGGCTT195040.14218599596245426No Hit
TATTGCACTTGTCCCGGCCTGTAG193490.14105603137189948No Hit
TGTAAACATCCCCGACTGGAAGC192510.14034160214690355No Hit
TTATAAAGCAATGAGACTGATT190910.13917518708568571No Hit
GGAATACCGGGTGCTGTAGGCTTT190820.13910957623849224No Hit
TTCACAGTGGCTAAGTTCTGC187890.13697357865763707No Hit
AGCAGCATTGTACAGGGCTATGT184110.13421792307550992No Hit
TAGCAGCACATAATGGTTTGT181780.13251933114261144No Hit
CAACGGAATCCCAAAAGCAGCT181170.13207463540052214No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC179990.13121440429287398No Hit
CAAGTCCTTCTGATCGAGGCCC176220.12846603880487947No Hit
TCGTTTCCCGGGCGGCGCACCA174690.1273506544025899No Hit
CAAAGTGCTGTTCGTGCAGGTAG174650.12732149402605947No Hit
TATTGCACTTGTCCCGGCCTGTT170040.12396076063092556No Hit
CCTCGACACAAGGGTTTGT169410.12350148470057105No Hit
TCAGGCTCAGTCCCCTCCCGATA169170.12332652244138836No Hit
GTCAGTTTGTCAAATACCCCAA166320.12124884561359409No Hit
TCAGGCTCAGTCCCCTCCCGA164660.12003868998758059No Hit
TCTCCCAACCCTTGTACCAGTGA159970.11661963583938582No Hit
AGCAGCATTGTACAGGGCTATG152380.11108645439273371No Hit
TACCTGGTTGATCCTGCC151940.1107656902508988No Hit
TTCAGGATGGCCGAGCGGTCTAAGG151100.11015332234375944No Hit
TCCTGTCTGAGCGTCGCT148220.10805377523356732No Hit
GGAATACCGGGTGCTGTAGGCTT147650.10763823986800847No Hit
CCCCCACTGCTAAATTTGACTGGCTA146250.10661762668944287No Hit
CTCCCACTGCTTCACTTGACTAGCCT145880.10634789320653625No Hit
ACCACAGGGTAGAACCACGGAC144080.10503567626266617No Hit
CACTAGATTGTGAGCTCCTGGAA143760.1048023932504226No Hit
TAGCAGCACGTAAATATTGGCGA143660.10472949230909648No Hit
TATTGCACTTGTCCCGGCCTGTAAA140800.10264452538716962No Hit
AGAATTACTCTGAGACCT140190.1021998296450803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)