FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10622862
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10174079.577522517001539No Hit
CCCCCCACTGCTAAATTTGACTG9484238.928130667611045No Hit
TTCAAGTAATCCAGGATAGGCT4218263.9709261025889258No Hit
CCCCCACTGCTAAATTTGACTG3621843.409476655161293No Hit
TAGCAGCACGTAAATATTGGCG3525253.3185501233095187No Hit
AAGCTGCCAGTTGAAGAACTGT2909272.7386875589648065No Hit
AACATTCAACGCTGTCGGTGAGT2570672.4199410667294745No Hit
AACATTCAACGCTGTCGGTGAG2119201.9949426058627138No Hit
TAGCTTATCAGACTGATGTTGAC1758421.6553166180639456No Hit
TGAGGTAGTAGGTTGTATAGTT1436981.3527239645963582No Hit
TAGCTTATCAGACTGATGTTGACT1405221.3228261837534931No Hit
TATTGCACTTGTCCCGGCCTGTAT1328391.2505010419979097No Hit
GCCTGTCTGAGCGTCGCT1316791.2395811976094577No Hit
TATTGCACTTGTCCCGGCCTGTAA1263601.189509945624823No Hit
TAGCTTATCAGACTGATGTTGA1247791.1746269508160796No Hit
TTCAAGTAATTCAGGATAGGTT1173311.1045140189150533No Hit
TGAGGTAGTAGATTGTATAGTT954390.8984301970598885No Hit
TATTGCACTTGTCCCGGCCTGT910910.8574996079211046No Hit
TGTAAACATCCTTGACTGGAAGCT874550.8232715439586807No Hit
CATAAAGTAGAAAGCACTACT844010.7945222295083941No Hit
CAACGGAATCCCAAAAGCAGCTGT704930.6635970607544369No Hit
TCGTACGACTCTTAGCGG631180.5941713259571667No Hit
CTCCCACTGCTTCACTTGACTAG613140.5771890852013327No Hit
AGCAGCATTGTACAGGGCTATGA589330.5547751632281395No Hit
TATTGCACTTGTCCCGGCCTGTA587600.5531466002288272No Hit
CCCCCACTGCTAAATTTGACTGGCTT539210.5075939045428624No Hit
CCCATAAAGTAGAAAGCACTAC529360.4983214504716337No Hit
TTCTCACTACTGCACTTGACTAG522660.4920142989714072No Hit
TGTAAACATCCCCGACTGGAAGCT468460.4409922674322607No Hit
TCCTGTCTGAGCGTCGCT455140.4284532737034521No Hit
ACTGGACTTGGAGTCAGAAGGC416570.39214479111184913No Hit
CATTGCACTTGTCTCGGTCTGA410480.38641187280791184No Hit
CTTCTCACTACTGCACTTGACTAG378300.35611871828891306No Hit
TGAGGTAGTAGTTTGTACAGTT370360.3486442730781968No Hit
CCCCCCACTGCTAAATTTGACTGGCT359130.3380727340711006No Hit
ACCCCCCACTGCTAAATTTGACTG347420.32704933943413744No Hit
CTCCCACTGCTTCACTTGACTAGCCT295090.27778766211968114No Hit
TGTAAACATCCTACACTCTCAGCT294790.27750525235101425No Hit
AACCGGGCGGAAACACCA293250.2760555488718577No Hit
TAGCAGCACGTAAATATTGGC290040.2730337643471223No Hit
CCCCCCACTGCTAAATTTGACTGG282600.2660300020841841No Hit
CAACGGAATCCCAAAAGCAGCT248490.23392001138676188No Hit
TGTCAGTTTGTCAAATACCCCAA240970.22684093985217918No Hit
TAGCTTATCAGACTGATGTTGACA232100.21849102435859566No Hit
TGAGGTAGTAGTTTGTGCTGTT229780.21630705548090523No Hit
TGTCAGTTTGTCAAATACCCCA227150.21383126317559242No Hit
CCCATAAAGTAGAAAGCACT218340.20553782963574221No Hit
CAAAGAATTCTCCTTTTGGGCT216620.20391868029538554No Hit
AACGGAATCCCAAAAGCAGCTG213210.20070862259153888No Hit
TTCAAGTAATCCAGGATAGGCTT213130.2006333133198944No Hit
TTCAAGTAATCCAGGATAGGC212990.20050152209451652No Hit
CAACACCAGTCGATGGGCTGT212040.19960722449373813No Hit
CGCGACCTCAGATCAGACGTGG205420.19337538226515605No Hit
TGATGTAGTAGGTTGTATAGTT191610.1803751192475248No Hit
AGCTCGGTCTGAGGCCCCTCAGT189760.17863359234074583No Hit
TCCCACTGCTTCACTTGACTAG187950.1769297200697891No Hit
TTCACAGTGGCTAAGTTCTGC180140.16957765242549513No Hit
TATTGCACTTGTCCCGGCCTGTT174920.16466372245069172No Hit
CACCCGGCTGTGTGCACATGTGC169000.15909083634899898No Hit
AACATTCAACGCTGTCGGTGAGTT168890.15898728610048782No Hit
TTCACAGTGGCTAAGTTCCGC168390.15851660315270968No Hit
ACCGGGTGCTGTAGGCTT165430.15573016010186333No Hit
TGAGGTAGTAGGTTGTATAGT157420.1481898192784581No Hit
CTACGCCTGTCTGAGCGTCGCT153460.14446201033205552No Hit
CGCGACCTCAGATCAGACGTGGCG151860.14295582489916558No Hit
TAGCAGCACGTAAATATTGGCGT149360.14060241016027508No Hit
CACTAGATTGTGAGCTCCTGGA148530.13982107646696343No Hit
TTATAAAGCAATGAGACTGATT147850.13918094765798522No Hit
TATTGCACTTGTCCCGGCCTGTAG146170.13759945295345077No Hit
ACCGGGTGCTGTAGGCTTA146150.13758062563553966No Hit
ATCACATTGCCAGGGATTTCC144480.13600854458996078No Hit
AGCAGCATTGTACAGGGCTATGT142950.13456825476975978No Hit
CAAAGAATTCTCCTTTTGGGCTT137350.12929660575464502No Hit
ACCGGGTGCTGTAGGCTTT137280.1292307101419561No Hit
TGAGGTAGTAGGTTGTGTGGTT136430.12843054913073332No Hit
CGCCTGTCTGAGCGTCGCT132550.12477804945597523No Hit
TCGTTTCCCGGCCAATGCACCA132480.12471215384328631No Hit
CCCCCACTGCTAAATTTGACTGGCT132290.12453329432313062No Hit
AGAGGTAGTAGGTTGCATAGTT131670.12394964746788577No Hit
CACTAGATTGTGAGCTCCTGGAA130220.12258466691932926No Hit
TGATGTAGTAGATTGTATAGTT128420.1208902083073281No Hit
CCCCCCACTGCTAAATTTGACTGT126270.11886627163188225No Hit
CAAAGTGCTGTTCGTGCAGGTAG124790.11747305010645907No Hit
TGTAAACATCCCCGACTGGAAGC120020.11298273478465597No Hit
TTCACCACCTTCTCCACCCAGC119560.11254970647270011No Hit
TTCACAGTGGCTAAGTTCCG114940.10820059603523043No Hit
TATTGCACTTGTCCCGGCCTGTAAT114810.10807821846880812No Hit
TAGCAGCACATCATGGTTTACA112830.10621431399560684No Hit
CCCATAAAGTAGAAAGCACTA112680.10607310911127339No Hit
GGAATACCGGGTGCTGTAGGCTTT110310.1038420719388052No Hit
TATTGCACTTGTCCCGGCCTGTTT109540.10311722019922692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)