FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8501335
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT218622525.716255152867166No Hit
CAACGGAATCCCAAAAGCAGCTG3924944.616851353346268No Hit
CCCCCCACTGCTAAATTTGACTG2609143.0690944422258384No Hit
TTCAAGTAATCCAGGATAGGCT2547042.996047091427405No Hit
TAGCAGCACGTAAATATTGGCG2391592.8131934572628885No Hit
AAGCTGCCAGTTGAAGAACTGT2216192.606872920547185No Hit
CCCCCACTGCTAAATTTGACTG1641411.9307673441877071No Hit
CATAAAGTAGAAAGCACTACT1133041.332778910606393No Hit
AACATTCAACGCTGTCGGTGAGT1103661.2982196325635915No Hit
TAGCTTATCAGACTGATGTTGA1068021.2562968051488383No Hit
TAGCTTATCAGACTGATGTTGAC1040431.2238430787635117No Hit
AACATTCAACGCTGTCGGTGAG919181.081218420401031No Hit
TGAGGTAGTAGGTTGTATAGTT890181.0471061309782523No Hit
TAGCTTATCAGACTGATGTTGACT780410.9179852340838233No Hit
AACCGGGCGGAAACACCA704110.8282346243266498No Hit
TATTGCACTTGTCCCGGCCTGT643910.7574222166283295No Hit
TGAGGTAGTAGATTGTATAGTT616300.7249449645261598No Hit
TTCAAGTAATTCAGGATAGGTT593140.6977021844216231No Hit
TGTAAACATCCTTGACTGGAAGCT515760.6066811859549117No Hit
TATTGCACTTGTCCCGGCCTGTAT489380.5756507654386046No Hit
TCGTACGACTCTTAGCGG480990.565781727222842No Hit
TATTGCACTTGTCCCGGCCTGTAA453430.5333632894127804No Hit
TTCTCACTACTGCACTTGACTAG422060.49646320254407106No Hit
ACCGGGTGCTGTAGGCTT408270.4802422207806186No Hit
CGCCTGTCTGAGCGTCGCT349250.4108178303760527No Hit
CCCATAAAGTAGAAAGCACTAC341500.40170161509927554No Hit
CATTGCACTTGTCTCGGTCTGA315730.37138872894668895No Hit
TGAGGTAGTAGTTTGTACAGTT290840.3421109743352073No Hit
CAACACCAGTCGATGGGCTGT290630.34186395430835276No Hit
CAACGGAATCCCAAAAGCAGCTGT277350.32624287832440435No Hit
TATTGCACTTGTCCCGGCCTGTA275590.3241726152421943No Hit
ACCGGGTGCTGTAGGCTTT259080.3047521359880537No Hit
AGCAGCATTGTACAGGGCTATGA249790.29382444051434276No Hit
ACCGGGTGCTGTAGGCTTA231400.27219254387693226No Hit
GGAATACCGGGTGCTGTAGGCTTT230110.2706751351405397No Hit
CTCCCACTGCTTCACTTGACTAG228020.2682166977304153No Hit
TGAGAACTGAATTCCATGGGTT217190.2554775220597706No Hit
GGAATACCGGGTGCTGTAGGCTT208780.24558495812716474No Hit
TCCTGTCTGAGCGTCGCT199830.23505719983978987No Hit
CTACGCCTGTCTGAGCGTCGCT195330.2297639135500483No Hit
CTTCTCACTACTGCACTTGACTAG194320.22857586484946188No Hit
CCCATAAAGTAGAAAGCACT187380.22041244110483824No Hit
TGTAAACATCCCCGACTGGAAGCT182360.21450748617717097No Hit
TTCACAGTGGCTAAGTTCCGC176730.20788499688578324No Hit
ACTGGACTTGGAGTCAGAAGGC173800.2044384793682404No Hit
TGAGGTAGTAGTTTGTGCTGTT163690.19254622950395436No Hit
TAGCAGCACGTAAATATTGGC159080.1871235517715747No Hit
TTCACAGTGGCTAAGTTCTGC157590.18537088586674916No Hit
TTCACAGTGGCTAAGTTCCG154750.18203023407500116No Hit
GCCGGTCTGAGCGTCGCT151760.17851313940692845No Hit
TTCAAGTAATCCAGGATAGGC143760.16910285266961012No Hit
ATCCCGGACGAGCCCCCA143180.16842060688115454No Hit
TGTAAACATCCTACACTCTCAGCT140360.16510348080624984No Hit
TGTCAGTTTGTCAAATACCCCA137960.16228039478505435No Hit
TTATAAAGCAATGAGACTGATT129240.1520231822413774No Hit
TGATGTAGTAGGTTGTATAGTT128370.15099981355869402No Hit
TACGCCTGTCTGAGCGTCGCT128080.15065869066446622No Hit
CCCCCACTGCTAAATTTGACTGGCTT127170.14958827054809629No Hit
TCCCACTGCTTCACTTGACTAG124500.14644758734951627No Hit
TGAGGTAGTAGGTTGTATAGT119010.1399897780760316No Hit
TGTCAGTTTGTCAAATACCCCAA118290.13914285226967293No Hit
ACGCCTGTCTGAGCGTCGCT115810.13622566338110426No Hit
AACGGAATCCCAAAAGCAGCTG108340.12743880814013328No Hit
GCCTGTCTGAGCGTCGCTT105520.12412168206522858No Hit
CAACGGAATCCCAAAAGCAGCT100610.11834611858019947No Hit
TTCACAGTGGCTAAGTTCTG99360.11687576127749348No Hit
TAGCTTATCAGACTGATGTTGACA97820.1150642810805597No Hit
TTCAAGTAATCCAGGATAGGCTT97690.11491136392107829No Hit
ATCCCACCGCTGCCACCA95060.11181773215618489No Hit
TTAATGCTAATCGTGATAGGGGTT94230.1108414149071881No Hit
CGCGACCTCAGATCAGACGTGG93700.11021798341084078No Hit
TATTGCACTTGTCCCGGCCTGTT92660.1089946461349894No Hit
AGCTCGGTCTGAGGCCCCTCAGT91720.1078889374433545No Hit
CATTATTACTTTTGGTACGCG90610.10658326015855156No Hit
TGAGGTAGTAGATTGTATAGT89780.10560694290955479No Hit
TGATGTAGTAGATTGTATAGTT88950.10463062566055802No Hit
CAAAGAATTCTCCTTTTGGGCT85200.10021955375244006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)