FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9605264
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT109768311.427931600838875No Hit
CAACGGAATCCCAAAAGCAGCTG9159179.535573410579866No Hit
CCCCCCACTGCTAAATTTGACTG7049627.339329767510814No Hit
CCCCCACTGCTAAATTTGACTG3690123.841768430310713No Hit
TTCAAGTAATCCAGGATAGGCT3055263.18081835127072No Hit
TAGCAGCACGTAAATATTGGCG2477822.5796479930171623No Hit
AACATTCAACGCTGTCGGTGAGT1899201.977249141720623No Hit
AAGCTGCCAGTTGAAGAACTGT1709121.779357652220699No Hit
AACATTCAACGCTGTCGGTGAG1495631.5570941100629823No Hit
TAGCTTATCAGACTGATGTTGA1191981.2409653706550907No Hit
TAGCTTATCAGACTGATGTTGAC1145071.1921275667175832No Hit
TAGCTTATCAGACTGATGTTGACT1031791.0741922345913657No Hit
CATAAAGTAGAAAGCACTACT901590.9386415615437534No Hit
TGAGGTAGTAGGTTGTATAGTT819790.8534799251743627No Hit
TATTGCACTTGTCCCGGCCTGTAA666990.6944004870662587No Hit
TCGTACGACTCTTAGCGG643690.6701429549463711No Hit
AACCGGGCGGAAACACCA597580.6221380276481729No Hit
TATTGCACTTGTCCCGGCCTGTAT584340.6083539192676015No Hit
TTCAAGTAATTCAGGATAGGTT545860.5682925529168172No Hit
CAACGGAATCCCAAAAGCAGCTGT537740.5598388550278264No Hit
TATTGCACTTGTCCCGGCCTGT517270.5385276240194959No Hit
CCCCCACTGCTAAATTTGACTGGCTT463100.48213146458025513No Hit
TGAGGTAGTAGATTGTATAGTT448740.4671813289046506No Hit
TTCTCACTACTGCACTTGACTAG442960.4611637951856398No Hit
CCCATAAAGTAGAAAGCACTAC441420.4595605076549692No Hit
AGCAGCATTGTACAGGGCTATGA432050.44980544001705736No Hit
TGTAAACATCCTTGACTGGAAGCT419660.43690626306575225No Hit
CATTGCACTTGTCTCGGTCTGA379340.3949292804445562No Hit
CTCCCACTGCTTCACTTGACTAG356560.3712131181402198No Hit
CGCCTGTCTGAGCGTCGCT354250.36880818684421374No Hit
CAACACCAGTCGATGGGCTGT348590.3629155846210994No Hit
ACTGGACTTGGAGTCAGAAGGC316950.32997531353641085No Hit
CTTCTCACTACTGCACTTGACTAG313550.32643558781934573No Hit
CCCATAAAGTAGAAAGCACT303250.3157123010882366No Hit
TATTGCACTTGTCCCGGCCTGTA299930.3122558630351024No Hit
ACCGGGTGCTGTAGGCTT260190.2708827159774057No Hit
CAACGGAATCCCAAAAGCAGCT246220.2563386076634645No Hit
TGTAAACATCCCCGACTGGAAGCT230930.24042025289466276No Hit
CCCCCCACTGCTAAATTTGACTGGCT225060.2343090205537297No Hit
ACCCCCCACTGCTAAATTTGACTG210510.21916107667628915No Hit
CCCCCCACTGCTAAATTTGACTGG208920.21750573435566165No Hit
CTACGCCTGTCTGAGCGTCGCT206750.2152465564715348No Hit
TGATGTAGTAGGTTGTATAGTT191300.19916162637487111No Hit
TAGCAGCACGTAAATATTGGC180640.18806354515607274No Hit
AACGGAATCCCAAAAGCAGCTG180530.1879490246181677No Hit
ACCGGGTGCTGTAGGCTTT174840.18202518952107927No Hit
TGTAAACATCCTACACTCTCAGCT174590.18176491557129507No Hit
CTCCCACTGCTTCACTTGACTAGCCT168220.1751331353307936No Hit
TGAGGTAGTAGTTTGTACAGTT163480.17019834124288516No Hit
ACCGGGTGCTGTAGGCTTA156340.16276491723704833No Hit
TTCACAGTGGCTAAGTTCCGC153620.15993313666339623No Hit
CAAAGAATTCTCCTTTTGGGCT152190.1584443696706306No Hit
GGAATACCGGGTGCTGTAGGCTTT149030.1551545069453583No Hit
TTCACAGTGGCTAAGTTCTGC148630.15473806862570358No Hit
AACATTCAACGCTGTCGGTGAGTT144660.15060491830313044No Hit
AGCTCGGTCTGAGGCCCCTCAGT144650.1505945073451391No Hit
TTCAAGTAATCCAGGATAGGC143000.14887669927656336No Hit
TTCAAGTAATCCAGGATAGGCTT140050.1458054666691098No Hit
TTCACAGTGGCTAAGTTCCG132490.13793478242763552No Hit
CACCCGGCTGTGTGCACATGTGC131490.1368936866284987No Hit
TGAGGTAGTAGTTTGTGCTGTT126000.13117807069123763No Hit
TAGCTTATCAGACTGATGTTGACA122890.12794026275592216No Hit
GGAATACCGGGTGCTGTAGGCTT121310.12629533139328603No Hit
TCCCACTGCTTCACTTGACTAG120490.12544163283799384No Hit
GACCCGCGGGCGCTCTCCAGTCCT118970.1238591672233059No Hit
TTCACAGTGGCTAAGTTCTG111460.11604053777178848No Hit
CGCGACCTCAGATCAGACGTGG109720.11422903108129043No Hit
CCCATAAAGTAGAAAGCACTA107620.11204272990310313No Hit
TGATGTAGTAGATTGTATAGTT105350.10967944243906258No Hit
GCCGGTCTGAGCGTCGCT105110.10942957944726973No Hit
CCCCCACTGCTAAATTTGACTGGCT104140.10841971652210704No Hit
ATCACATTGCCAGGGATTTCC103900.10816985353031422No Hit
CACTAGATTGTGAGCTCCTGGA102160.10635834683981617No Hit
CAAAGAATTCTCCTTTTGGGCTT102110.10630629204985932No Hit
TTATAAAGCAATGAGACTGATT99190.10326629231637986No Hit
CGCGACCTCAGATCAGACGTGGCG98450.10249588142501863No Hit
TACGCCTGTCTGAGCGTCGCT98310.10235012801313946No Hit
ACTGGACTTGGAGTCAGAAGGCT97900.10192327873549338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)