FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8532702
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7206208.445390451934218No Hit
CAACGGAATCCCAAAAGCAGCTG5652186.624138520248334No Hit
AACATTCAACGCTGTCGGTGAGT3202743.7534886370108786No Hit
AAGCTGCCAGTTGAAGAACTGT2453212.8750681788722963No Hit
TAGCAGCACGTAAATATTGGCG2272032.6627321568244153No Hit
TTCAAGTAATCCAGGATAGGCT2097822.4585647078733093No Hit
AACATTCAACGCTGTCGGTGAG1984092.3252775029527575No Hit
CCCCCACTGCTAAATTTGACTG1713522.008179823929161No Hit
TAGCTTATCAGACTGATGTTGAC1442931.6910587056714272No Hit
TATTGCACTTGTCCCGGCCTGTAA1424031.6689086294118791No Hit
TATTGCACTTGTCCCGGCCTGTAT1212011.420429308324608No Hit
TAGCTTATCAGACTGATGTTGACT1104811.2947950133498158No Hit
CGCCTGTCTGAGCGTCGCT917391.075145950251163No Hit
TGTAAACATCCTTGACTGGAAGCT901321.0563125256220127No Hit
TGAGGTAGTAGGTTGTATAGTT837460.9814710510222905No Hit
GCCTGTCTGAGCGTCGCT763120.894347417734734No Hit
TATTGCACTTGTCCCGGCCTGT732940.858977613421868No Hit
AGCAGCATTGTACAGGGCTATGA701130.8216975115268295No Hit
ACTGGACTTGGAGTCAGAAGGC603630.7074312451085248No Hit
TATTGCACTTGTCCCGGCCTGTA602970.706657750382001No Hit
TAGCTTATCAGACTGATGTTGA590190.6916800797684016No Hit
TGTAAACATCCCCGACTGGAAGCT474370.5559434748805244No Hit
TGAGGTAGTAGATTGTATAGTT450490.5279570293208411No Hit
TTCAAGTAATTCAGGATAGGTT411800.48261383088264426No Hit
TACGCCTGTCTGAGCGTCGCT339110.39742393441139745No Hit
CAACGGAATCCCAAAAGCAGCTGT328010.38441515946531357No Hit
AACATTCAACGCTGTCGGTGAGTT318770.37358623329397883No Hit
CATAAAGTAGAAAGCACTACT317430.37201580460679395No Hit
CTACGCCTGTCTGAGCGTCGCT258650.303127895477892No Hit
CACCCGGCTGTGTGCACATGTGC257210.30144027062002166No Hit
GCGTTCGCGCTTTCCCCTG230930.2706411169638879No Hit
CCCATAAAGTAGAAAGCACTAC227360.2664572136704177No Hit
CCCCCACTGCTAAATTTGACTGGCTT225660.2644648787687652No Hit
CATTGCACTTGTCTCGGTCTGA220980.25898009798068655No Hit
TCCTGTCTGAGCGTCGCT213810.25057713254254044No Hit
TGTAAACATCCTACACTCTCAGCT206860.24243199867990234No Hit
GGAATACCGGGTGCTGTAGGCTTT206840.24240855944576523No Hit
TAGCTTATCAGACTGATGTTGACA201400.23603308776047727No Hit
TTCTCACTACTGCACTTGACTAG199590.2339118370710708No Hit
TGTCAGTTTGTCAAATACCCCAA197280.23120460552823716No Hit
TGAGGTAGTAGTTTGTACAGTT195640.2292825883289959No Hit
ACGCCTGTCTGAGCGTCGCT192700.22583702091084393No Hit
CAACGGAATCCCAAAAGCAGCT184830.21661368227789976No Hit
CTTCTCACTACTGCACTTGACTAG184700.2164613272560087No Hit
ACCGGGTGCTGTAGGCTT181040.2121719474089216No Hit
ACTGGACTTGGAGTCAGAAGGCA180340.2113515742141235No Hit
CGCGACCTCAGATCAGACGTGGCG180220.21121093880930095No Hit
TAGCAGCACGTAAATATTGGC175310.20545660682864583No Hit
CAAAGAATTCTCCTTTTGGGCT174930.20501126138004117No Hit
TATTGCACTTGTCCCGGCCTGTAG167580.1963973428346613No Hit
ACCCCCCACTGCTAAATTTGACTG165270.19369011129182762No Hit
CCCCCCACTGCTAAATTTGACTGG161920.18976403957386537No Hit
CTCCCACTGCTTCACTTGACTAG154700.18130247605037653No Hit
GGAATACCGGGTGCTGTAGGCTT149880.1756536206233383No Hit
TCGTACGACTCTTAGCGG147790.1732042206560126No Hit
CAAAGAATTCTCCTTTTGGGCTT147100.17239556707828307No Hit
AACGGAATCCCAAAAGCAGCTG144670.16954770013062687No Hit
TTCAAGTAATCCAGGATAGGCTT144600.16946566281114706No Hit
TGAGGTAGTAGTTTGTGCTGTT138600.1624338925700206No Hit
CCCCCCACTGCTAAATTTGACTGGCT137140.16072282847801317No Hit
ACCGGGTGCTGTAGGCTTA136490.15996105336855782No Hit
ACCGGGTGCTGTAGGCTTT134590.15773432612553445No Hit
AGCAGCATTGTACAGGGCTATGT134130.15719522374038142No Hit
TATTGCACTTGTCCCGGCCTGTT132940.15580058930922466No Hit
CCAAGTCCTTCTGATCGAGGCCC131940.15462862760237026No Hit
CACTAGATTGTGAGCTCCTGGAA131530.15414812330255995No Hit
CAACACCAGTCGATGGGCTGT128460.15055020086251694No Hit
ACTGGACTTGGAGTCAGAAGGCT127880.14987046307254137No Hit
CACTAGATTGTGAGCTCCTGGA125690.14730386693453024No Hit
CCCATAAAGTAGAAAGCACT124880.14635457795197818No Hit
TATTGCACTTGTCCCGGCCTGTAAT124600.14602642867405893No Hit
AACCGGGCGGAAACACCA122140.14314340287519708No Hit
TGAGGTAGTAGGTTGTGTGGTT120590.14132686222957275No Hit
TGTAAACATCCCCGACTGGAAGC119480.1400259847349644No Hit
TTATAAAGCAATGAGACTGATT117670.13790473404555792No Hit
TATTGCACTTGTCCCGGCCTGTAAA115360.13519750250272422No Hit
ACTGGACTTGGAGTCAGAAGG112750.13213868244783422No Hit
TGTCAGTTTGTCAAATACCCCA110050.12897438583932733No Hit
CGCGACCTCAGATCAGACGTGG109230.1280133772397067No Hit
TAGCAGCACGTAAATATTGGCGT108420.12706408825715465No Hit
ACCACAGGGTAGAACCACGGAC106920.12530614569687304No Hit
CCTCGACACAAGGGTTTGT102420.12003231801602822No Hit
CAAAGTGCTGTTCGTGCAGGTAG99110.11615312476634014No Hit
TTCAAGTAATCCAGGATAGGC97710.11451237837674397No Hit
TTAATGCTAATCGTGATAGGGGTT97450.11420766833296182No Hit
AGCTCGGTCTGAGGCCCCTCAGT97030.11371544441608297No Hit
GACCCGCGGGCGCTCTCCAGTCCT96840.11349277169178064No Hit
ATCACATTGCCAGGGATTTCCA95270.11165279181201923No Hit
AACATTCAACGCTGTCGGTGAGA95030.11137152100237416No Hit
CTCCCACTGCTTCACTTGACTAGCCT93990.11015268082724557No Hit
AGCAGCATTGTACAGGGCTATG93540.1096252980591611No Hit
TTCACAGTGGCTAAGTTCCG92590.10851193443764942No Hit
CCCCCCACTGCTAAATTTGACTGTA90980.10662507608961382No Hit
CAAGTCCTTCTGATCGAGGCCC90650.10623832872635186No Hit
TTCACAGTGGCTAAGTTCCGC88640.10388268569557452No Hit
CTGCGTTCGCGCTTTCCCCTG85970.10075354793827325No Hit
ATGCACCTGGGCAAGGATTCTGA85900.10067151061879344No Hit
AACATTCATTGCTGTCGGTGGGTT85580.10029648287260003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)