FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8428115
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG101305712.019971250985542No Hit
CCCCCCACTGCTAAATTTGACTG8073509.579247554168399No Hit
TTCAAGTAATCCAGGATAGGCT2814633.3395723717580976No Hit
AACATTCAACGCTGTCGGTGAGT2203702.614700914735976No Hit
CCCCCACTGCTAAATTTGACTG1898672.2527813158695627No Hit
TAGCAGCACGTAAATATTGGCG1636221.9413830969321133No Hit
AAGCTGCCAGTTGAAGAACTGT1289391.5298675919823117No Hit
AACATTCAACGCTGTCGGTGAG1250801.4840803667249438No Hit
CAACGGAATCCCAAAAGCAGCTGT708590.8407455285078573No Hit
ACTGGACTTGGAGTCAGAAGGC620730.736499205338323No Hit
TATTGCACTTGTCCCGGCCTGTAA588220.6979259300567209No Hit
TCGTACGACTCTTAGCGG560520.6650597434894991No Hit
AGCAGCATTGTACAGGGCTATGA507910.6026377191103823No Hit
TATTGCACTTGTCCCGGCCTGTAT488420.5795127380203047No Hit
CATAAAGTAGAAAGCACTACT487930.5789313506044946No Hit
CCCATAAAGTAGAAAGCACTAC481390.5711716083608256No Hit
CAACGGAATCCCAAAAGCAGCT434450.5154770669360824No Hit
CACCCGGCTGTGTGCACATGTGC427880.5076817295445066No Hit
TATTGCACTTGTCCCGGCCTGT418430.49646925795388414No Hit
CTCCCACTGCTTCACTTGACTAG382280.45357710472626445No Hit
TAGCTTATCAGACTGATGTTGAC369170.4380220250910198No Hit
TTCAAGTAATTCAGGATAGGTT368390.43709655124544455No Hit
CACTAGATTGTGAGCTCCTGGA357150.42376023582971994No Hit
TAGCTTATCAGACTGATGTTGA352540.41829044810138444No Hit
CACTAGATTGTGAGCTCCTGGAA320410.3801680446932677No Hit
CCCCCACTGCTAAATTTGACTGGCTT305560.36254844647943224No Hit
CATTGCACTTGTCTCGGTCTGA302380.35877536080131794No Hit
AGCTCGGTCTGAGGCCCCTCAGT300100.35607012956040585No Hit
TGAGGTAGTAGGTTGTATAGTT284460.337513192451693No Hit
CCCATAAAGTAGAAAGCACT268310.31835113782856544No Hit
CTACGCCTGTCTGAGCGTCGCT264680.31404412493185013No Hit
ACCGGGTGCTGTAGGCTTA264620.31397293463603665No Hit
CAAAGAATTCTCCTTTTGGGCT264460.3137830938472007No Hit
TTAATGCTAATCGTGATAGGGGTT254930.30247570186216016No Hit
TATTGCACTTGTCCCGGCCTGTA250390.2970889694789404No Hit
CTTCTCACTACTGCACTTGACTAG248560.2949176654566294No Hit
GACCCGCGGGCGCTCTCCAGTCCT247940.29418203239989016No Hit
TAGCTTATCAGACTGATGTTGACT246600.2925921157933891No Hit
ATCCCGGACGAGCCCCCA246530.2925090604482734No Hit
TTCTCACTACTGCACTTGACTAG227710.27017903766144624No Hit
CGCGACCTCAGATCAGACGTGGCG219900.26091243415639204No Hit
CCCCCCACTGCTAAATTTGACTGGCT213360.25315269191272305No Hit
CGCCTGTCTGAGCGTCGCT213040.2527730103350512No Hit
TGTAAACATCCTACACTCTCAGCT203200.24109780182164103No Hit
TCGTTTCCCGGGCGGCGCACCA194240.23046671764682852No Hit
AACGGAATCCCAAAAGCAGCTG193290.22933953796311513No Hit
ACCGGGTGCTGTAGGCTT185070.2195864674366688No Hit
TGAGGTAGTAGATTGTATAGTT183410.21761686925249596No Hit
CAAAGAATTCTCCTTTTGGGCTT182770.2168575060971522No Hit
CCCCCCACTGCTAAATTTGACTGG182300.21629984877994662No Hit
AACATTCAACGCTGTCGGTGAGTT180860.2145912816804232No Hit
ACCCCCCACTGCTAAATTTGACTG171190.20311777900515118No Hit
TGAGAACTGAATTCCATGGGTT167930.199249772932619No Hit
ACTGGACTTGGAGTCAGAAGGCT167650.1989175515521561No Hit
TGTAAACATCCTTGACTGGAAGCT162760.19311554244335774No Hit
ACTGGACTTGGAGTCAGAAGGCA162690.193032487098242No Hit
TACGCCTGTCTGAGCGTCGCT162240.19249855987964093No Hit
GGAATACCGGGTGCTGTAGGCTTT160010.18985265388524006No Hit
CGCGACCTCAGATCAGACGTGGC159330.1890458305326873No Hit
ACCGGGTGCTGTAGGCTTT155980.18507103901643487No Hit
TTCAAGTAATCCAGGATAGGCTT150910.1790554590201961No Hit
CCCCCCACTGCTAAATTTGACTGT143150.16984818076165312No Hit
TGTAAACATCCCCGACTGGAAGCT139750.16581406399888943No Hit
TCGTTTCCCGGCCAATGCACCA137910.16363089492727614No Hit
CAACGGAATCCCAAAAGCAGCTGA136130.16151891615147634No Hit
AACCGGGCGGAAACACCA128170.15207433690688843No Hit
CCCCCCACTGCTAAATTTGACTGTA127360.1511132679134065No Hit
CAACACCAGTCGATGGGCTGT127320.15106580771619751No Hit
CCCATAAAGTAGAAAGCACTA123470.14649776373483275No Hit
CGCGACCTCAGATCAGACGTGG121500.14416034902229027No Hit
GCCTGTCTGAGCGTCGCT117610.13954484484371654No Hit
TTCAAGTAATCCAGGATAGGC116180.13784814279349536No Hit
ACTGGACTTGGAGTCAGAAGG114500.13585481451071799No Hit
TTCACAGTGGCTAAGTTCCG112910.13396827167166087No Hit
GGAATACCGGGTGCTGTAGGCTT112240.13317331336841037No Hit
AGCAGCATTGTACAGGGCTATGT107920.12804761206984006No Hit
TAGCAGCACGTAAATATTGGC105100.12470166816660666No Hit
AGCTACATCTGGCTACTGGGTCTC100800.11959969696664081No Hit
ACCACAGGGTAGAACCACGGAC100100.11876914351548358No Hit
CGGGCCGCCGGTGAAATACCACTAC98120.11641986375363887No Hit
ACGCCTGTCTGAGCGTCGCT93310.11071277503925847No Hit
TTCACAGTGGCTAAGTTCCGC93060.11041614880670232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)