FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10079188
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6850046.796222076619664No Hit
CCCCCCACTGCTAAATTTGACTG5099225.059157543246539No Hit
AACATTCAACGCTGTCGGTGAGT4439764.404878646970372No Hit
AAGCTGCCAGTTGAAGAACTGT3627373.598871258279933No Hit
TTCAAGTAATCCAGGATAGGCT3382903.3563219576815113No Hit
TAGCAGCACGTAAATATTGGCG2831542.8092937645373817No Hit
AACATTCAACGCTGTCGGTGAG2326642.3083605544414887No Hit
CCCCCACTGCTAAATTTGACTG1879671.8649022123607577No Hit
TGAGGTAGTAGGTTGTATAGTT1259981.2500808596883004No Hit
CGCCTGTCTGAGCGTCGCT1259401.2495054165077584No Hit
TATTGCACTTGTCCCGGCCTGTAA1071651.0632304903926784No Hit
TAGCTTATCAGACTGATGTTGAC1037391.0292396570041158No Hit
TATTGCACTTGTCCCGGCCTGT1033981.0258564479598953No Hit
TAGCTTATCAGACTGATGTTGACT943670.9362559761758587No Hit
TATTGCACTTGTCCCGGCCTGTAT913370.9061940307096167No Hit
GCCTGTCTGAGCGTCGCT876040.8691573170378407No Hit
TTAATGCTAATCGTGATAGGGGTT872200.8653474863252874No Hit
TAGCTTATCAGACTGATGTTGA849220.8425480306548504No Hit
CATAAAGTAGAAAGCACTACT817680.8112558273543464No Hit
TGTAAACATCCTTGACTGGAAGCT694230.6887757228062419No Hit
AGCAGCATTGTACAGGGCTATGA629410.6244649866636082No Hit
TGAGGTAGTAGATTGTATAGTT591220.5865750296551666No Hit
TATTGCACTTGTCCCGGCCTGTA587040.5824278701816059No Hit
ACTGGACTTGGAGTCAGAAGGC542640.5383767025677069No Hit
TTCAAGTAATTCAGGATAGGTT498440.4945239636367533No Hit
CAACGGAATCCCAAAAGCAGCTGT442690.4392119682656976No Hit
ACCGGGTGCTGTAGGCTT434230.4308184349771033No Hit
CCCATAAAGTAGAAAGCACTAC433950.43054063482097965No Hit
TGTAAACATCCCCGACTGGAAGCT405210.40202643308171254No Hit
TCGTACGACTCTTAGCGG397600.3944762216956366No Hit
TCCTGTCTGAGCGTCGCT386720.3836817013434019No Hit
AACCGGGCGGAAACACCA350890.3481332027937171No Hit
TACGCCTGTCTGAGCGTCGCT350670.3479149312424771No Hit
CCCATAAAGTAGAAAGCACT348850.34610923022767315No Hit
AACATTCAACGCTGTCGGTGAGTT343770.3410691416808576No Hit
CATTGCACTTGTCTCGGTCTGA327630.32505594696715645No Hit
GGAATACCGGGTGCTGTAGGCTTT308070.3056496217750874No Hit
TGAGGTAGTAGTTTGTACAGTT297040.2947062799106436No Hit
ACCGGGTGCTGTAGGCTTT293830.29152149954936846No Hit
CTACGCCTGTCTGAGCGTCGCT292140.28984477717847906No Hit
ACGCCTGTCTGAGCGTCGCT272200.27006143748881356No Hit
CACCCGGCTGTGTGCACATGTGC266790.26469394161513804No Hit
ACCGGGTGCTGTAGGCTTA262120.2600606318683608No Hit
ATCCCGGACGAGCCCCCA238670.23679486879300196No Hit
CAACACCAGTCGATGGGCTGT236490.23463199614889613No Hit
GGAATACCGGGTGCTGTAGGCTT231070.2292545788410733No Hit
TTCTCACTACTGCACTTGACTAG230950.22913552163130602No Hit
TGTAAACATCCTACACTCTCAGCT222630.22088088842077358No Hit
TGTCAGTTTGTCAAATACCCCAA205910.20429225052653052No Hit
TGAGGTAGTAGTTTGTGCTGTT192460.19094792159844623No Hit
CAACGGAATCCCAAAAGCAGCT188690.1872075409249237No Hit
AACGGAATCCCAAAAGCAGCTG179800.17838738596799664No Hit
TAGCAGCACGTAAATATTGGC174950.17357549040656847No Hit
TTCAAGTAATCCAGGATAGGCTT174240.172871068582112No Hit
TGAGGTAGTAGGTTGTGTGGTT162750.16147134074689348No Hit
TATTGCACTTGTCCCGGCCTGTT158460.15721504549771273No Hit
TTCACAGTGGCTAAGTTCCG158050.15680826669767445No Hit
TTCAAGTAATCCAGGATAGGC156720.15548871595608693No Hit
AGCAGCATTGTACAGGGCTATGT144020.14288849458904826No Hit
TTATAAAGCAATGAGACTGATT141780.14066609334005875No Hit
ACTGGACTTGGAGTCAGAAGGCA141340.14022955023757866No Hit
CTTCTCACTACTGCACTTGACTAG140450.13934654259847123No Hit
ACTGGACTTGGAGTCAGAAGGCT140390.1392870139935876No Hit
GCGTTCGCGCTTTCCCCTG139620.13852306356424743No Hit
TGAGAACTGAATTCCATGGGTT139400.1383047920130074No Hit
CCCCCACTGCTAAATTTGACTGGCTT138850.1377591131349073No Hit
ACCACAGGGTAGAACCACGGAC138710.13762021305684544No Hit
AGCTCGGTCTGAGGCCCCTCAGT137520.13643956239331978No Hit
TTCACAGTGGCTAAGTTCCGC134680.1336218750954938No Hit
ATCCCACCGCTGCCACCA134240.13318533199301374No Hit
CAAAGAATTCTCCTTTTGGGCT133020.13197491702704622No Hit
CTCCCACTGCTTCACTTGACTAG132070.13103238078305512No Hit
TGAATACCGGGTGCTGTAGGCTTT131710.13067520915375325No Hit
CACTAGATTGTGAGCTCCTGGA128190.12718286433391263No Hit
CCCACCACTGCTAAATTTGACTG127630.12662726402166524No Hit
CAACGGAATCCAAAAAGCAGCTG126880.12588315646061965No Hit
CCACCCACTGCTAAATTTGACTG126180.1251886560703104No Hit
TATTGCACTTGTCCCGGCCTGTAG124030.1230555477286464No Hit
CACCCCACTGCTAAATTTGACTG123920.12294641195302637No Hit
TAGCTTATCAGACTGATGTTGACA123900.12292656908473182No Hit
CGCGACCTCAGATCAGAC123850.12287696191399546No Hit
CAAAGTGCTGTTCGTGCAGGT123740.12276782613837543No Hit
ACTGGACTTGGAGTCAGAAGG121870.12091251795283509No Hit
TGAGGTAGTAGGTTGTATAGT120920.11996998170884401No Hit
TTCACAGTGGCTAAGTTCTGC120390.11944414569903847No Hit
TCGTTTCCCGGCCAATGCACCA118330.11740033026469988No Hit
AACATTCAACGCTGTCGGTGAGA116170.11525730048888858No Hit
AGCAGCATTGTACAGGGCTATG115080.11417586416683566No Hit
TGTAAACATCCCCGACTGGAAGC114550.11365002815703011No Hit
TGTCAGTTTGTCAAATACCCCA111130.1102568976786622No Hit
TAGCAGCACGTAAATATTGGCGT110680.10981043314203486No Hit
CGCGACCTCAGATCAGACGTGG110150.10928459713222931No Hit
CCCCCCACTGCTAAATTTGACTGG110080.10921514709319836No Hit
CAAAGAATTCTCCTTTTGGGCTT109740.10887781833219105No Hit
AACATTCAACGCTGTCGGTGA105120.10429411575615019No Hit
CGCGACCTCAGATCAGACGTGGCG103040.10223045745351708No Hit
TGTAAACATCCTACACTCAGCT101560.10076208519972046No Hit
TTAATGCTAATCGTGATAGGGGTTT101360.10056365651677497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)