FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7570421
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG113889915.044064260098613No Hit
CCCCCCACTGCTAAATTTGACTG6994089.238693594451352No Hit
CCCCCACTGCTAAATTTGACTG2439553.222476002325366No Hit
TTCAAGTAATCCAGGATAGGCT2383333.1482132895911596No Hit
AACATTCAACGCTGTCGGTGAG2138662.8250212240508157No Hit
TCGTACGACTCTTAGCGG1862112.459717894156745No Hit
TAGCAGCACGTAAATATTGGCG1313111.734527049420369No Hit
TTAATGCTAATCGTGATAGGGGTT1107421.4628248547868077No Hit
AAGCTGCCAGTTGAAGAACTGT1033081.3646268813848001No Hit
TGAGGTAGTAGATTGTATAGTT933391.232943319796878No Hit
TGAGGTAGTAGGTTGTATAGTT835541.103690270329748No Hit
AACATTCAACGCTGTCGGTGAGT809811.0697027285536695No Hit
TATTGCACTTGTCCCGGCCTGTAA790441.0441163047603297No Hit
TGAGAACTGAATTCCATGGGTT768401.0150029965308403No Hit
TATTGCACTTGTCCCGGCCTGT762961.007817134608498No Hit
TAGCTTATCAGACTGATGTTGAC665490.8790660387315316No Hit
TATTGCACTTGTCCCGGCCTGTAT659960.8717612930641506No Hit
GGAATACCGGGTGCTGTAGGCTTT517130.6830927896876541No Hit
TATTGCACTTGTCCCGGCCTGTA502320.6635298089762776No Hit
CAACGGAATCCCAAAAGCAGCTGT495730.6548248769784402No Hit
TAGCTTATCAGACTGATGTTGA455520.6017102615561275No Hit
TAGCTTATCAGACTGATGTTGACT451100.5958717487442244No Hit
GGAATACCGGGTGCTGTAGGCTT380530.50265368332884No Hit
CAACGGAATCCCAAAAGCAGCT365490.4827868886023644No Hit
CACCCGGCTGTGTGCACATGTGC344930.4556285575135121No Hit
AGCAGCATTGTACAGGGCTATGA338750.44746520702085124No Hit
TGAGGTAGTAGTTTGTACAGTT333990.44117757783880185No Hit
TTCTCACTACTGCACTTGACTAG333880.44103227548375445No Hit
TGTAAACATCCTTGACTGGAAGCT333870.4410190661787502No Hit
CCCCCACTGCTAAATTTGACTGGCTT312820.41321347914468687No Hit
TTCAAGTAATTCAGGATAGGTT284960.37641235540269163No Hit
CATTGCACTTGTCTCGGTCTGA256520.33884509197044654No Hit
ACTGGACTTGGAGTCAGAAGGC253760.3351993237892582No Hit
AACGGAATCCCAAAAGCAGCTG248150.3277889036818428No Hit
TGTAAACATCCCCGACTGGAAGCT238240.314698482422576No Hit
TCGTTTCCCGGCCAATGCACCA231150.30533308517452334No Hit
ACCGGGTGCTGTAGGCTT225190.2974603393919572No Hit
CATAAAGTAGAAAGCACTACT218940.28920452376426625No Hit
CTCCCACTGCTTCACTTGACTAG204030.2695094500028466No Hit
TTAATGCTAATCGTGATAGGGGTTT201610.26631279819180464No Hit
GCCTGTCTGAGCGTCGCT199170.2630897277707541No Hit
CCCATAAAGTAGAAAGCACTAC192580.2543847957729167No Hit
CTTCTCACTACTGCACTTGACTAG192510.2542923306378866No Hit
CAAAGAATTCTCCTTTTGGGCT183640.24257567709906752No Hit
TGAGGTAGTAGTTTGTGCTGTT180990.23907521127292655No Hit
ACCGGGTGCTGTAGGCTTT177710.23474255923151433No Hit
TGAGAACTGAATTCCATAGGCTGT175480.2317968842155542No Hit
ACCCCCCACTGCTAAATTTGACTG174980.2311364189653389No Hit
CCCATAAAGTAGAAAGCACT168720.2228673940326436No Hit
CACTAGATTGTGAGCTCCTGGA149230.19712245857925206No Hit
CACTAGATTGTGAGCTCCTGGAA142370.18806087534629845No Hit
CCCCCCACTGCTAAATTTGACTGG142250.18790236368624677No Hit
TAGCAGCACGTAAATATTGGC141980.18754571245113053No Hit
ACCGGGTGCTGTAGGCTTA138330.182724316124559No Hit
AGCTCGGTCTGAGGCCCCTCAGT135290.17870868740325008No Hit
CTCCCACTGCTTCACTTGACTAGCCT124220.16408598676348382No Hit
TATTGCACTTGTCCCGGCCTGTT120760.15951556723199412No Hit
GACCCGCGGGCGCTCTCCAGTCCT118880.15703221789118466No Hit
CCCATAAAGTAGAAAGCACTA115730.1528712868148284No Hit
TAGCTTATCAGACTGATGTTGACA113630.15009733276392423No Hit
AATGCACCCGGGCAAGGATTCT110880.14646477388774018No Hit
ACTGGACTTGGAGTCAGAAGGCT106720.14096970300594908No Hit
TGTAAACATCCTACACTCTCAGCT106120.14017714470569073No Hit
TGAGAACTGAATTCCATGGGTTG105610.13950347015047115No Hit
TTAATGCTAATCGTGATAGGGGT105410.13923928405038505No Hit
TATTGCACTTGTCCCGGCCTGTAG100950.13334793401846476No Hit
TGAGGTAGTAGATTGTATAGT97640.12897565406203962No Hit
TGATGTAGTAGATTGTATAGTT95900.12667723499129044No Hit
TTCAAGTAATCCAGGATAGGC95010.12550160684590725No Hit
CCCCCCACTGCTAAATTTGACTGGCT94140.12435239731053266No Hit
AGCTACATTGTCTGCTGGGTTT93120.12300504820009349No Hit
ACTGGACTTGGAGTCAGAAGGCA88550.11696839581312585No Hit
TTCACAGTGGCTAAGTTCCGC86190.11385099983210972No Hit
TTCACAGTGGCTAAGTTCTGC84850.1120809529615328No Hit
TGATGTAGTAGGTTGTATAGTT84850.1120809529615328No Hit
AGCTACATCTGGCTACTGGGTCTC83620.11045620844600319No Hit
ATGCACCTGGGCAAGGATTCTGA83480.11027127817594291No Hit
AATCCTTGGAACCTAGGTGTGAGT82860.10945230126567598No Hit
CAACACCAGTCGATGGGCTGT82170.1085408592203789No Hit
TGTAAACATCCCCGACTGGA82050.10838234756032723No Hit
TTCACAGTGGCTAAGTTCTG80740.10665192860476319No Hit
CTCGTACGACTCTTAGCGG77620.10253062544341987No Hit
CAAAGAATTCTCCTTTTGGGCTT76850.10151350895808832No Hit
CAAAGAATTCTCCTTTTGGGC76540.10110402050295486No Hit
GGAATACCGGGTGCTGTAGGCTTA76110.10053602038776971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)