FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8237392
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG121735214.77836674520285No Hit
CCCCCCACTGCTAAATTTGACTG5473456.644639468414275No Hit
TTCAAGTAATCCAGGATAGGCT2959453.5927026418070183No Hit
TCGTACGACTCTTAGCGG2332772.8319278723168693No Hit
CCCCCACTGCTAAATTTGACTG2297752.7894144166017596No Hit
AACATTCAACGCTGTCGGTGAG2293902.784740607221315No Hit
TAGCAGCACGTAAATATTGGCG2137402.5947532908473945No Hit
AAGCTGCCAGTTGAAGAACTGT1535951.8646071475049386No Hit
TGAGGTAGTAGATTGTATAGTT1203111.460547221742027No Hit
TAGCTTATCAGACTGATGTTGAC980081.1897940513210978No Hit
TGAGGTAGTAGGTTGTATAGTT939901.1410164770597298No Hit
AACATTCAACGCTGTCGGTGAGT936401.1367675594411435No Hit
TATTGCACTTGTCCCGGCCTGT904351.0978596138195196No Hit
TAGCTTATCAGACTGATGTTGA773410.9389015358259022No Hit
TATTGCACTTGTCCCGGCCTGTAA692150.840253808486958No Hit
TAGCTTATCAGACTGATGTTGACT576530.6998938498981231No Hit
TATTGCACTTGTCCCGGCCTGTAT560170.6800331949723893No Hit
CAACGGAATCCCAAAAGCAGCTGT520280.6316076738851326No Hit
TATTGCACTTGTCCCGGCCTGTA483870.5874067908872129No Hit
AGCAGCATTGTACAGGGCTATGA467520.5675582757261036No Hit
TGAGAACTGAATTCCATGGGTT445080.5403166439086546No Hit
TGAGGTAGTAGTTTGTACAGTT443700.5386413563904693No Hit
TTCAAGTAATTCAGGATAGGTT442740.5374759389865141No Hit
TTCTCACTACTGCACTTGACTAG440450.5346959328874965No Hit
TGTAAACATCCTTGACTGGAAGCT424980.5159157170133459No Hit
CATAAAGTAGAAAGCACTACT392380.4763400843373728No Hit
CAACGGAATCCCAAAAGCAGCT380090.4614203136138234No Hit
CACCCGGCTGTGTGCACATGTGC369010.44796945440984237No Hit
CATTGCACTTGTCTCGGTCTGA353390.4290071420663239No Hit
ACTGGACTTGGAGTCAGAAGGC323870.3931705568947065No Hit
CCCCCACTGCTAAATTTGACTGGCTT317840.3858502788261139No Hit
AACGGAATCCCAAAAGCAGCTG281280.3414672993588262No Hit
CCCATAAAGTAGAAAGCACTAC276930.3361865017471549No Hit
CTCCCACTGCTTCACTTGACTAG276360.33549453516355665No Hit
TTAATGCTAATCGTGATAGGGGTT270340.32818639685958856No Hit
TGTAAACATCCCCGACTGGAAGCT259920.3155367621208266No Hit
CCCATAAAGTAGAAAGCACT256130.3109357913281291No Hit
TGAGGTAGTAGTTTGTGCTGTT244290.2965623100126836No Hit
TCGTTTCCCGGCCAATGCACCA239500.290747362757533No Hit
CTTCTCACTACTGCACTTGACTAG216150.26240101235925156No Hit
TAGCAGCACGTAAATATTGGC216030.26225533518375715No Hit
ACCGGGTGCTGTAGGCTT207610.2520336533699015No Hit
GCCTGTCTGAGCGTCGCT195020.23674968970761617No Hit
TTCACAGTGGCTAAGTTCCGC172110.2089374889528142No Hit
CAAAGAATTCTCCTTTTGGGCT171900.208682553895699No Hit
TGATGTAGTAGATTGTATAGTT163390.19835161420022257No Hit
ACCGGGTGCTGTAGGCTTT151890.18439088488201122No Hit
TAGCTTATCAGACTGATGTTGACA148630.1804333216144139No Hit
TTCACAGTGGCTAAGTTCTGC146340.17765331551539615No Hit
TGAGGTAGTAGATTGTATAGT136890.16618123794521372No Hit
CACTAGATTGTGAGCTCCTGGA136630.1658656040649759No Hit
TTCAAGTAATCCAGGATAGGC136470.1656713678309834No Hit
GGAATACCGGGTGCTGTAGGCTTT134410.1631705763183299No Hit
TATTGCACTTGTCCCGGCCTGTT132720.16111895609678403No Hit
CCCATAAAGTAGAAAGCACTA132040.16029345210231588No Hit
TGTCAGTTTGTCAAATACCCCA128030.1554254064878787No Hit
TGATGTAGTAGGTTGTATAGTT127860.15521903048926167No Hit
AATGCACCCGGGCAAGGATTCT127520.15480627849202758No Hit
ACCCCCCACTGCTAAATTTGACTG126170.1531674102677158No Hit
CAACACCAGTCGATGGGCTGT124060.1506059199319396No Hit
AGCTCGGTCTGAGGCCCCTCAGT123680.1501446088762074No Hit
TGGCTCAGTTCAGCAGGAAC122420.14861499853351642No Hit
TGTAAACATCCCCGACTGGA122240.14839648277027487No Hit
ACTGGACTTGGAGTCAGAAGGCT121450.14743744136493686No Hit
CTCCCACTGCTTCACTTGACTAGCCT117660.14283647057223936No Hit
CACTAGATTGTGAGCTCCTGGAA115700.14045707670583116No Hit
CCCCCCACTGCTAAATTTGACTGG114780.13934021836037427No Hit
GGAATACCGGGTGCTGTAGGCTT114440.13892746636314018No Hit
TGAGAACTGAATTCCATAGGCTGT114020.13841759624890984No Hit
AACCGGGCGGAAACACCA109570.13301540099099327No Hit
TGTAAACATCCTACACTCTCAGCT109020.13234771393664402No Hit
CCCCCCACTGCTAAATTTGACTGGCT108430.13163146782379664No Hit
CAAAGTGCTGTTCGTGCAGGTAG107590.130611727595336No Hit
TTCACAGTGGCTAAGTTCTG104380.12671486315086133No Hit
ACCGGGTGCTGTAGGCTTA104060.12632639068287632No Hit
TGAGGTAGTAAGTTGTATTGTT99180.12040218554610488No Hit
TTCAAGTAATCCAGGATAGGCTT98110.11910323073128001No Hit
ATCACATTGCCAGGGATTTCC97330.11815632909056653No Hit
TTCACAGTGGCTAAGTTCCG96850.11757362038858901No Hit
ACTGGACTTGGAGTCAGAAGGCA95260.11564339781328847No Hit
AGCAGCATTGTACAGGGCTATGT91250.11077535219885129No Hit
TGAGGTAGTAGTTTGTGCTGTTA87730.10650215505101615No Hit
GACCCGCGGGCGCTCTCCAGTCCT87580.10632005858164817No Hit
CAAAGAATTCTCCTTTTGGGCTT86970.10557953293955173No Hit
GTCAGGATGGCCGAGCGGTCTAAGG85570.1038799658921173No Hit
TGTAAACATCCCCGACTGGAAGC85240.10347935365950776No Hit
TCCCACTGCTTCACTTGACTAG82870.10060222944349377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)