FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12194628
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG122075610.010604669531535No Hit
CAACGGAATCCCAAAAGCAGCTG7936376.508087003555992No Hit
GGAATACCGGGTGCTGTAGGCTTT4754753.8990529272397647No Hit
AACATTCAACGCTGTCGGTGAGT3834173.1441467505199836No Hit
TTCAAGTAATCCAGGATAGGCT3478112.85216572412049No Hit
GGAATACCGGGTGCTGTAGGCTT3284442.693349891444003No Hit
CCCCCACTGCTAAATTTGACTG3058812.508325797228091No Hit
TAGCAGCACGTAAATATTGGCG2957212.4250104226221576No Hit
AAGCTGCCAGTTGAAGAACTGT2735072.2428482443252884No Hit
TATTGCACTTGTCCCGGCCTGTAT2373671.9464882405596955No Hit
TATTGCACTTGTCCCGGCCTGTAA2335911.9155237863754435No Hit
AACATTCAACGCTGTCGGTGAG2174531.7831868261992085No Hit
TGAGGTAGTAGGTTGTATAGTT1702541.396139349228201No Hit
TAGCTTATCAGACTGATGTTGACT1581231.296661119962003No Hit
TAGCTTATCAGACTGATGTTGAC1399521.147652884532435No Hit
TATTGCACTTGTCCCGGCCTGT1324331.0859945871247567No Hit
TAGCTTATCAGACTGATGTTGA1154560.9467775482778154No Hit
TGAATACCGGGTGCTGTAGGCTTT1119050.9176581688264702No Hit
TATTGCACTTGTCCCGGCCTGTA919660.7541517461623266No Hit
TGAGGTAGTAGATTGTATAGTT907120.743868529650925No Hit
TGTAAACATCCTTGACTGGAAGCT874710.7172912531649183No Hit
GGAATACCGGGTGCTGTAGGCTTA796280.652976048141854No Hit
TGAATACCGGGTGCTGTAGGCTT762190.6250211158552765No Hit
AGCAGCATTGTACAGGGCTATGA722430.5924165952417737No Hit
ACTGGACTTGGAGTCAGAAGGC721920.5919983783023147No Hit
TGTAAACATCCCCGACTGGAAGCT670630.5499388747241818No Hit
CATAAAGTAGAAAGCACTACT603830.4951606559872101No Hit
TTCAAGTAATTCAGGATAGGTT599040.4912326968891548No Hit
TCGTACGACTCTTAGCGG581570.47690671662965034No Hit
CAACGGAATCCCAAAAGCAGCTGT519550.42604825665858764No Hit
GGAATACCGGGTGCTGTAGGCT510920.41897137001637114No Hit
CCCATAAAGTAGAAAGCACTAC485290.39795391872552405No Hit
TTCTCACTACTGCACTTGACTAG437240.3585513227627772No Hit
CTTCTCACTACTGCACTTGACTAG399880.32791488186437506No Hit
CTCCCACTGCTTCACTTGACTAG374800.30734844884157186No Hit
TGAGGTAGTAGTTTGTACAGTT357740.29335868220006384No Hit
CATTGCACTTGTCTCGGTCTGA340650.279344314562117No Hit
CGCCTGTCTGAGCGTCGCT303270.24869147299942237No Hit
CCCATAAAGTAGAAAGCACT301290.24706780723446425No Hit
CACCCGGCTGTGTGCACATGTGC297530.243984482347473No Hit
AGCTCGGTCTGAGGCCCCTCAGT286860.23523472794742079No Hit
CAACGGAATCCCAAAAGCAGCT282200.23141337316726676No Hit
AACATTCAACGCTGTCGGTGAGTT281480.23082294925273655No Hit
TATTGCACTTGTCCCGGCCTGTAG280890.23033912965610756No Hit
TATTGCACTTGTCCCGGCCTGTT259870.21310203148468326No Hit
ATCCCGGACGAGCCCCCA253770.2080998288754688No Hit
CGCGACCTCAGATCAGACGTGGCG252520.2070747873571871No Hit
ACCGGGTGCTGTAGGCTTA250770.20563972923159282No Hit
CACTAGATTGTGAGCTCCTGGA250420.20535271760647394No Hit
TGTAAACATCCTACACTCTCAGCT249860.20489349900628373No Hit
ACCCCCCACTGCTAAATTTGACTG245930.20167076847280624No Hit
CACTAGATTGTGAGCTCCTGGAA236250.19373284695523307No Hit
CCCCCACTGCTAAATTTGACTGGCTT234320.19215018285100618No Hit
TTCAAGTAATCCAGGATAGGCTT230780.18924726527123253No Hit
CAAAGAATTCTCCTTTTGGGCT227600.186639559648724No Hit
TATTGCACTTGTCCCGGCCTGTTT225550.1849584915587421No Hit
GACCCGCGGGCGCTCTCCAGTCCT225070.18456487561572194No Hit
CCCCCCACTGCTAAATTTGACTGGCT223780.18350703276885527No Hit
CCCCCCACTGCTAAATTTGACTGG217710.17852943115607953No Hit
TGAGGTAGTAGTTTGTGCTGTT208410.17090312226006402No Hit
AACGGAATCCCAAAAGCAGCTG203350.16675375419405988No Hit
TGTAAACATCCCCGACTGGAAGC192760.15806960245117768No Hit
TGAATACCGGGTGCTGTAGGCTTA192570.15791379614039888No Hit
ACTGGACTTGGAGTCAGAAGGCT191140.15674114864348465No Hit
ACTGGACTTGGAGTCAGAAGGCA190720.15639673469334203No Hit
CAACACCAGTCGATGGGCTGT190090.15588011376812808No Hit
TAGCTTATCAGACTGATGTTGACA188540.15460906228545881No Hit
CCCCCCACTGCTAAATTTGACTGT188070.1542236466745849No Hit
TATTGCACTTGTCCCGGCCTGTAAT185160.1518373500200252No Hit
CTACGCCTGTCTGAGCGTCGCT183380.15037769089799213No Hit
TAGCAGCACGTAAATATTGGC183140.15018088292648205No Hit
CAAAGAATTCTCCTTTTGGGCTT175110.1435960162130407No Hit
ACCGGGTGCTGTAGGCTT174370.14298919163421797No Hit
TGAGGTAGTAGGTTGTGTGGTT172300.14129172287994352No Hit
TTCAAGTAATCCAGGATAGGC167670.13749496909622827No Hit
AGCAGCATTGTACAGGGCTATGT165400.1356334936990288No Hit
TACGCCTGTCTGAGCGTCGCT161320.13228775818335747No Hit
TGAGAACTGAATTCCATGGGTT158930.13032787880040295No Hit
ACCGGGTGCTGTAGGCTTT158710.13014747149318537No Hit
TGAGGTAGTAGGTTGTATAGT152340.12492385991602203No Hit
GCCTGTCTGAGCGTCGCT151230.12401362304778794No Hit
CGCGACCTCAGATCAGACGTGG150890.12373481175481532No Hit
ACTGGACTTGGAGTCAGAAGG146600.12021686926407267No Hit
CCCCCCACTGCTAAATTTGACTGTA146120.11982325332105252No Hit
TATTGCACTTGTCCCGGCCTGTAAA143990.11807658257390057No Hit
TATTGCACTTGTCCCGGCCTGA141940.11639551448391866No Hit
TCGTTTCCCGGGCGGCGCACCA138720.1137550075328251No Hit
TGTCAGTTTGTCAAATACCCCAA130490.10700613417645868No Hit
TTCACAGTGGCTAAGTTCCG129430.10613689896895584No Hit
GGAATACCGGGTGCTGTAGGCTA127530.10457883586116772No Hit
TTCACAGTGGCTAAGTTCTGC125430.10285676611045455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)