FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11885594
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG147820212.436921537114594No Hit
CAACGGAATCCCAAAAGCAGCTG7054475.9353112684145195No Hit
AACATTCAACGCTGTCGGTGAGT3885843.26936962511087No Hit
CCCCCACTGCTAAATTTGACTG3484492.931691928901492No Hit
TTCAAGTAATCCAGGATAGGCT2752122.3155090103195515No Hit
AAGCTGCCAGTTGAAGAACTGT2456962.0671747663600155No Hit
TAGCAGCACGTAAATATTGGCG2449552.060940328266303No Hit
AACATTCAACGCTGTCGGTGAG2290341.9269882514916798No Hit
TAGCTTATCAGACTGATGTTGAC2253521.8960095725968764No Hit
TATTGCACTTGTCCCGGCCTGTAA1960761.6496945798417817No Hit
TATTGCACTTGTCCCGGCCTGTAT1637681.3778697135372453No Hit
TAGCTTATCAGACTGATGTTGACT1552291.3060264383925615No Hit
TGAGGTAGTAGGTTGTATAGTT1240581.043767774669066No Hit
TTAATGCTAATCGTGATAGGGGTT1094180.9205934511981478No Hit
TGTAAACATCCTTGACTGGAAGCT1092200.9189275689544838No Hit
AGCAGCATTGTACAGGGCTATGA934580.7863132461028032No Hit
ACTGGACTTGGAGTCAGAAGGC911140.76659189267276No Hit
TAGCTTATCAGACTGATGTTGA880380.7407118230691709No Hit
CGCCTGTCTGAGCGTCGCT766330.6447553231247846No Hit
TATTGCACTTGTCCCGGCCTGTA753510.6339691562743941No Hit
TATTGCACTTGTCCCGGCCTGT697500.5868448812907457No Hit
TGTAAACATCCCCGACTGGAAGCT694790.5845648101390641No Hit
TGAGGTAGTAGATTGTATAGTT584190.491510983801062No Hit
GCCTGTCTGAGCGTCGCT526710.44314991745469345No Hit
TTCAAGTAATTCAGGATAGGTT480700.4044391891562172No Hit
CAACGGAATCCCAAAAGCAGCTGT456060.3837082101239534No Hit
GGAATACCGGGTGCTGTAGGCTTT422050.3550937378476835No Hit
CCCCCACTGCTAAATTTGACTGGCTT401490.3377954858629699No Hit
AACATTCAACGCTGTCGGTGAGTT390220.3283134187487811No Hit
TACGCCTGTCTGAGCGTCGCT357490.3007758804482132No Hit
TAGCTTATCAGACTGATGTTGACA345270.29049452639893303No Hit
TGTAAACATCCTACACTCTCAGCT324820.27328882342775634No Hit
CCCCCCACTGCTAAATTTGACTGG321990.27090778971585266No Hit
TTCTCACTACTGCACTTGACTAG309200.2601468635055177No Hit
CTTCTCACTACTGCACTTGACTAG300110.2524989495686964No Hit
ACCCCCCACTGCTAAATTTGACTG290770.24464069696474572No Hit
CGCGACCTCAGATCAGACGTGGCG288360.24261303221361927No Hit
ACTGGACTTGGAGTCAGAAGGCA278010.2339050113944663No Hit
CCCCCCACTGCTAAATTTGACTGGCT277610.2335684695270594No Hit
CATAAAGTAGAAAGCACTACT277170.23319827347291186No Hit
GGAATACCGGGTGCTGTAGGCTT275170.23151556413587743No Hit
GCGTTCGCGCTTTCCCCTG275120.23147349640245157No Hit
CTACGCCTGTCTGAGCGTCGCT267850.22535684796233155No Hit
CAACGGAATCCCAAAAGCAGCT253210.21303941561523979No Hit
CACCCGGCTGTGTGCACATGTGC245370.2064431950140649No Hit
CCCATAAAGTAGAAAGCACTAC244530.20573645709251048No Hit
CATTGCACTTGTCTCGGTCTGA244330.20556818615880706No Hit
TAGCAGCACGTAAATATTGGC240920.20269916673916338No Hit
TTCAAGTAATCCAGGATAGGCTT224810.18914494302935134No Hit
TGAGGTAGTAGTTTGTACAGTT222780.1874369930522614No Hit
CCCCCCACTGCTAAATTTGACTGTA222560.18725189502518763No Hit
CAAAGAATTCTCCTTTTGGGCT216880.18247300050800994No Hit
TATTGCACTTGTCCCGGCCTGTAG215230.18108476530495657No Hit
ACTGGACTTGGAGTCAGAAGGCT214540.1805042305836797No Hit
CACTAGATTGTGAGCTCCTGGAA210820.1773743912167957No Hit
CTCCCACTGCTTCACTTGACTAG204190.1717962097645267No Hit
CCAAGTCCTTCTGATCGAGGCCC192530.16198601432961618No Hit
AGCAGCATTGTACAGGGCTATGT189800.1596891160845642No Hit
CAAAGAATTCTCCTTTTGGGCTT189780.15967228899119387No Hit
CACTAGATTGTGAGCTCCTGGA177870.14965175488915405No Hit
TATTGCACTTGTCCCGGCCTGTAAT177010.14892818987422923No Hit
AACGGAATCCCAAAAGCAGCTG175830.14793539136537898No Hit
CCCCCCACTGCTAAATTTGACTGT170500.1434509709821823No Hit
CGCGACCTCAGATCAGACGTGG167270.14073339540287175No Hit
AACCGGGCGGAAACACCA165310.13908434025257804No Hit
TGTAAACATCCCCGACTGGAAGC162730.13691364520780366No Hit
TATTGCACTTGTCCCGGCCTGTAAA161450.13583671123210164No Hit
TGAGGTAGTAGTTTGTGCTGTT158660.1334893317069387No Hit
AGCTCGGTCTGAGGCCCCTCAGT157980.13291721053234698No Hit
ACGCCTGTCTGAGCGTCGCT157590.13258908221162527No Hit
CAAGTCCTTCTGATCGAGGCCC154720.1301743943129809No Hit
ATCACATTGCCAGGGATTTCCA153500.12914794161738993No Hit
TGAGGTAGTAGGTTGTGTGGTT152530.12833182758892825No Hit
ATAGGCGATAGAAATTGAAACCT152140.12800369926820654No Hit
TAGCAGCACGTAAATATTGGCGT150630.12673325371874558No Hit
TATTGCACTTGTCCCGGCCTGTT146950.12363706853860228No Hit
ACTGGACTTGGAGTCAGAAGG144930.12193753210819755No Hit
GACCCGCGGGCGCTCTCCAGTCCT144670.12171877989438305No Hit
GTGCGGTAACGCGACCGATCCCGGA144530.12160099024079066No Hit
TCCTGTCTGAGCGTCGCT141330.11890865530153562No Hit
ACCGGGTGCTGTAGGCTTA141310.11889182820816527No Hit
TGTCAGTTTGTCAAATACCCCAA138950.11690623119046469No Hit
ACCGGGTGCTGTAGGCTT136690.11500476963961581No Hit
TTAATGCTAATCGTGATAGGGGTTT136060.11447471619844998No Hit
TTATAAAGCAATGAGACTGATT135120.11368384281004382No Hit
CTGCGTTCGCGCTTTCCCCTG132150.11118501944454774No Hit
ACCACAGGGTAGAACCACGGAC131040.11025111576249366No Hit
TGTAAACATCCTACACTCAGCT128550.10815614263788582No Hit
CCCATAAAGTAGAAAGCACT127470.10724747959588725No Hit
TGTCAGTTTGTCAAATACCCCA127390.10718017122240589No Hit
CCTCGACACAAGGGTTTGT126910.10677632098151763No Hit
ATGCACCTGGGCAAGGATTCTGA125440.10553952961879734No Hit
CAAAGTGCTGTTCGTGCAGGTAG122770.10329311265385643No Hit
TGAATACCGGGTGCTGTAGGCTTT122580.10313325526683816No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT121390.10213204321130268No Hit
ACCGGGTGCTGTAGGCTTT119770.10076904864830484No Hit
GGGCTACGCCTGTCTGAGCGTCGCT119180.10027264939387967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)