FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8666722
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8228339.494166306476659No Hit
CAACGGAATCCCAAAAGCAGCTG5631956.498362356609569No Hit
AACATTCAACGCTGTCGGTGAGT3251763.75200681411034No Hit
CCCCCACTGCTAAATTTGACTG2697803.1128262796475994No Hit
TTCAAGTAATCCAGGATAGGCT2614853.01711535226352No Hit
AAGCTGCCAGTTGAAGAACTGT2528052.9169621455493786No Hit
TAGCAGCACGTAAATATTGGCG1966582.2691162817960473No Hit
AACATTCAACGCTGTCGGTGAG1557361.7969423733679237No Hit
TGAGGTAGTAGGTTGTATAGTT1377591.5895167746236696No Hit
TAGCTTATCAGACTGATGTTGAC1026111.1839655177586175No Hit
TATTGCACTTGTCCCGGCCTGTAA1000591.1545195519136302No Hit
TAGCTTATCAGACTGATGTTGACT938281.0826238570938354No Hit
TAGCTTATCAGACTGATGTTGA907891.0475586963560155No Hit
TATTGCACTTGTCCCGGCCTGTAT905801.0451471732911244No Hit
TATTGCACTTGTCCCGGCCTGT900841.0394241329074592No Hit
GGAATACCGGGTGCTGTAGGCTTT788270.9095365006515729No Hit
CGCCTGTCTGAGCGTCGCT785790.9066749804597402No Hit
TTAATGCTAATCGTGATAGGGGTT689140.79515646169336No Hit
GCCTGTCTGAGCGTCGCT670080.7731642944125818No Hit
TGAGGTAGTAGATTGTATAGTT622540.7183107984772097No Hit
TGTAAACATCCTTGACTGGAAGCT621410.7170069606478666No Hit
CATAAAGTAGAAAGCACTACT567650.6549765874571724No Hit
GGAATACCGGGTGCTGTAGGCTT567360.6546419742089339No Hit
AGCAGCATTGTACAGGGCTATGA551880.6367805497857206No Hit
ACTGGACTTGGAGTCAGAAGGC537160.6197960428406496No Hit
TATTGCACTTGTCCCGGCCTGTA515320.59459620373193No Hit
TGTAAACATCCCCGACTGGAAGCT412750.47624695934633643No Hit
CAACGGAATCCCAAAAGCAGCTGT412680.4761661906312445No Hit
CCCATAAAGTAGAAAGCACTAC370970.4280395748242531No Hit
TGAATACCGGGTGCTGTAGGCTTT368690.42540882238982625No Hit
TTCAAGTAATTCAGGATAGGTT359940.41531273300332006No Hit
ACCGGGTGCTGTAGGCTT351850.4059781772162532No Hit
TCGTACGACTCTTAGCGG341440.3939667154432783No Hit
TACGCCTGTCTGAGCGTCGCT289290.3337940226997012No Hit
TCCTGTCTGAGCGTCGCT284690.32848636427936656No Hit
TGAGGTAGTAGTTTGTACAGTT280240.3233517816770862No Hit
CTACGCCTGTCTGAGCGTCGCT274450.31667105510018667No Hit
ACCGGGTGCTGTAGGCTTT270240.3118133938067934No Hit
CATTGCACTTGTCTCGGTCTGA266480.3074749599675633No Hit
CCCATAAAGTAGAAAGCACT263490.30402498199434574No Hit
TGAATACCGGGTGCTGTAGGCTT256620.2960981095274546No Hit
AACATTCAACGCTGTCGGTGAGTT255330.2946096574921868No Hit
ACCGGGTGCTGTAGGCTTA236850.2732867167078856No Hit
TTCTCACTACTGCACTTGACTAG235700.27195980210280196No Hit
TGTAAACATCCTACACTCTCAGCT210150.2424792210942038No Hit
AACCGGGCGGAAACACCA202470.2336177392098189No Hit
CCCCCACTGCTAAATTTGACTGGCTT200700.2315754445567771No Hit
ACGCCTGTCTGAGCGTCGCT199830.23057160481206157No Hit
TGAGGTAGTAGTTTGTGCTGTT190700.22003705668648424No Hit
TGAGGTAGTAGGTTGTGTGGTT175640.20266024455382323No Hit
CCCCCCACTGCTAAATTTGACTGG175100.20203717160882742No Hit
AACGGAATCCCAAAAGCAGCTG164350.18963340464826262No Hit
CAACACCAGTCGATGGGCTGT164030.18926417623641326No Hit
TGTCAGTTTGTCAAATACCCCAA162610.18762572515883166No Hit
TTCAAGTAATCCAGGATAGGCTT156990.1811411511757271No Hit
CTTCTCACTACTGCACTTGACTAG156690.18079499953961833No Hit
ATACATGATGATCTCAATCCAAC156460.1805296166186016No Hit
CAACGGAATCCCAAAAGCAGCT154980.17882193521379824No Hit
CCCCCCACTGCTAAATTTGACTGGCT153240.1768142557243673No Hit
TATTGCACTTGTCCCGGCCTGTT148280.17109121534070207No Hit
ATCCCGGACGAGCCCCCA143300.16534509818129622No Hit
CACCCGGCTGTGTGCACATGTGC141200.16292203672853472No Hit
ACTGGACTTGGAGTCAGAAGGCA139270.16069512786956822No Hit
CGCGACCTCAGATCAGACGTGG138400.15969128812485275No Hit
CGCGACCTCAGATCAGACGTGGCG137810.15901052324050546No Hit
CTCCCACTGCTTCACTTGACTAG136960.15802976027153057No Hit
TGAGGTAGTAGGTTGTATAGT133250.15374901837165192No Hit
GCGTTCGCGCTTTCCCCTG131750.152018260191108No Hit
TAGCTTATCAGACTGATGTTGACA129710.1496644290655683No Hit
ACTGGACTTGGAGTCAGAAGG127760.1474144434308612No Hit
CACTAGATTGTGAGCTCCTGGA126150.14555676298374404No Hit
ACTGGACTTGGAGTCAGAAGGCT125610.1449336900387482No Hit
TTCAAGTAATCCAGGATAGGC125260.14452984646328798No Hit
AGCAGCATTGTACAGGGCTATGT122050.14082602395692395No Hit
TTCACAGTGGCTAAGTTCCG118820.13709912467481938No Hit
TGTAAACATCCCCGACTGGAAGC117900.13603759299075244No Hit
CGCGACCTCAGATCAGAC116330.13422606609511648No Hit
GGAATACCGGGTGCTGTAGGCTTA116300.13419145093150558No Hit
ACCCCCCACTGCTAAATTTGACTG116210.13408760544067294No Hit
AGCTCGGTCTGAGGCCCCTCAGT115500.13326837990188217No Hit
TATTGCACTTGTCCCGGCCTGTAG112920.13029147583134662No Hit
TGAGAACTGAATTCCATGGGTT112400.12969147966209138No Hit
ACCACAGGGTAGAACCACGGAC111610.12877994702033826No Hit
TAGCAGCACGTAAATATTGGC111050.12813379729960186No Hit
CAAAGAATTCTCCTTTTGGGCT110300.1272684182093299No Hit
GACCCGCGGGCGCTCTCCAGTCCT106660.12306844502454331No Hit
CCCCCCACTGCTAAATTTGACTGTA103860.1198376964208613No Hit
CACTAGATTGTGAGCTCCTGGAA100630.11611079713875674No Hit
TTATAAAGCAATGAGACTGATT99130.1143800389582128No Hit
CAAAGTGCTGTTCGTGCAGGT97950.11301850918951825No Hit
TGTCAGTTTGTCAAATACCCCA97300.11226851397794922No Hit
AGCAGCATTGTACAGGGCTATG97030.11195697750545132No Hit
AGAGGTAGTAGGTTGCATAGTT95600.11030698803999944No Hit
CAAAGAATTCTCCTTTTGGGCTT91350.10540317319512497No Hit
TTAATGCTAATCGTGATAGGGGTTT91060.10506855994688649No Hit
TGTAAACATCCTACACTCAGCT91010.10501086800753504No Hit
TTCACAGTGGCTAAGTTCTGC90510.10443394861402039No Hit
CCCCCCACTGCTAAATTTGACTGT87200.10061474222895347No Hit
AACATTCAACGCTGTCGGTGAGA86950.10032628253219614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)