FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10285225
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG130701812.707723943812605No Hit
CCCCCCACTGCTAAATTTGACTG104506810.16086668011638No Hit
CCCCCACTGCTAAATTTGACTG4208114.091412681783821No Hit
TCGTACGACTCTTAGCGG3762963.6586073712534244No Hit
TTCAAGTAATCCAGGATAGGCT2939332.8578178892537593No Hit
AACATTCAACGCTGTCGGTGAG2401852.335243030658056No Hit
TAGCAGCACGTAAATATTGGCG1702411.655199570257335No Hit
TGAGGTAGTAGGTTGTATAGTT1302751.266622752540659No Hit
TGAGGTAGTAGATTGTATAGTT1268301.2331281036632646No Hit
TATTGCACTTGTCCCGGCCTGT1198491.1652540415985064No Hit
AAGCTGCCAGTTGAAGAACTGT1198081.1648554115247842No Hit
TTAATGCTAATCGTGATAGGGGTT1122201.091079679831992No Hit
TGAGAACTGAATTCCATGGGTT1058101.0287572707451709No Hit
TAGCTTATCAGACTGATGTTGAC1024760.9963418398722439No Hit
TATTGCACTTGTCCCGGCCTGTAA876120.8518238541208385No Hit
AACATTCAACGCTGTCGGTGAGT855390.8316687286860521No Hit
TAGCTTATCAGACTGATGTTGA819040.7963267697109203No Hit
TATTGCACTTGTCCCGGCCTGTAT791110.7691713112741821No Hit
ACCGGGTGCTGTAGGCTT712820.693052412562681No Hit
TAGCTTATCAGACTGATGTTGACT630830.6133361205029545No Hit
TATTGCACTTGTCCCGGCCTGTA626120.608756735997511No Hit
CAACGGAATCCCAAAAGCAGCTGT572970.5570806666844916No Hit
ACCGGGTGCTGTAGGCTTT540480.525491664013184No Hit
AGCAGCATTGTACAGGGCTATGA493870.4801742305102708No Hit
TTCTCACTACTGCACTTGACTAG475860.46266367532066627No Hit
GCCTGTCTGAGCGTCGCT457950.44525034697831106No Hit
TGAGGTAGTAGTTTGTACAGTT432550.420554727776981No Hit
TTCAAGTAATTCAGGATAGGTT401700.39056024540056244No Hit
CATTGCACTTGTCTCGGTCTGA401350.39021995143518984No Hit
ACTGGACTTGGAGTCAGAAGGC400880.3897629852531179No Hit
GGAATACCGGGTGCTGTAGGCTTT399370.38829485985965306No Hit
CAACGGAATCCCAAAAGCAGCT393950.3830251647387393No Hit
TGTAAACATCCTTGACTGGAAGCT392370.3814889805521999No Hit
CCCCCACTGCTAAATTTGACTGGCTT380560.3700064898920539No Hit
ACCGGGTGCTGTAGGCTTA361670.35164033844665527No Hit
GGAATACCGGGTGCTGTAGGCTT345080.33551040448799124No Hit
AACGGAATCCCAAAAGCAGCTG339490.3300754237267537No Hit
CACCCGGCTGTGTGCACATGTGC327350.3182720844706849No Hit
CATAAAGTAGAAAGCACTACT317230.3084327275290526No Hit
TGTAAACATCCCCGACTGGAAGCT294750.28657613226740297No Hit
CTCCCACTGCTTCACTTGACTAG267020.2596151275251635No Hit
CTTCTCACTACTGCACTTGACTAG239820.23316942507334548No Hit
CCCATAAAGTAGAAAGCACT239490.2328485764774227No Hit
TGAGAACTGAATTCCATAGGCTGT234010.22752054524815937No Hit
CCCATAAAGTAGAAAGCACTAC227030.22073411131015608No Hit
ACCCCCCACTGCTAAATTTGACTG220370.21425880328335062No Hit
CCCCCCACTGCTAAATTTGACTGG210500.20466251345984166No Hit
TCGTTTCCCGGCCAATGCACCA206340.20061787661426947No Hit
TAGCAGCACGTAAATATTGGC190750.1854602111280988No Hit
TTAATGCTAATCGTGATAGGGGTTT171630.16687043793402673No Hit
CACTAGATTGTGAGCTCCTGGA171230.1664815305450294No Hit
TATTGCACTTGTCCCGGCCTGTT166470.16185353261596125No Hit
CAACACCAGTCGATGGGCTGT159770.15533933385025608No Hit
AGCTCGGTCTGAGGCCCCTCAGT156500.15216001594520295No Hit
TGAGGTAGTAGATTGTATAGT149310.14516940562797606No Hit
TGATGTAGTAGGTTGTATAGTT148370.14425547326383234No Hit
TTAATGCTAATCGTGATAGGGGT146020.14197064235347306No Hit
TAGCTTATCAGACTGATGTTGACA145750.14170812986589987No Hit
TGATGTAGTAGATTGTATAGTT144650.14063863454615722No Hit
AACCGGGCGGAAACACCA143010.1390441142512682No Hit
CTCCCACTGCTTCACTTGACTAGCCT141180.13726486294660545No Hit
AATGCACCCGGGCAAGGATTCT140620.1367203926020092No Hit
TGTAAACATCCCCGACTGGA139110.1352522672085443No Hit
CACTAGATTGTGAGCTCCTGGAA135020.13127568915604665No Hit
CAAAGAATTCTCCTTTTGGGCT133980.13026452994465362No Hit
GACCCGCGGGCGCTCTCCAGTCCT133330.12963255543753296No Hit
TTCAAGTAATCCAGGATAGGC132770.12908808509293673No Hit
AGCTACATTGTCTGCTGGGTTT132080.12841721984691634No Hit
CCCATAAAGTAGAAAGCACTA131560.12791164024121982No Hit
CCCCCCACTGCTAAATTTGACTGGCT131510.12786302681759515No Hit
TGAGAACTGAATTCCATGGGTTG128490.12492677603066535No Hit
TTCACAGTGGCTAAGTTCCGC128320.12476149039034147No Hit
ACTGGACTTGGAGTCAGAAGGCA125540.12205858403681008No Hit
ACTGGACTTGGAGTCAGAAGGCT120810.11745975416191673No Hit
TTCACAGTGGCTAAGTTCTGC120250.11691528381732048No Hit
TGAGGTAGTAGTTTGTGCTGTT115400.11219978172572792No Hit
TTCACAGTGGCTAAGTTCTG113650.11049831189886464No Hit
CTCGTACGACTCTTAGCGG112790.1096621610125204No Hit
CAAAGTGCTGTTCGTGCAGGTAG112360.10924408556934827No Hit
TCCTGTACTGAGCTGCCCCGAGT111140.10805791803290643No Hit
TGATAACTGAATTCCATGGGTT110480.10741622084106085No Hit
TATTGCACTTGTCCCGGCCTGTAG110050.10699814539788872No Hit
GGGAATACCGGGTGCTGTAGGCTT108760.10574391906837234No Hit
TGGCTCAGTTCAGCAGGAAC108180.10518000335432623No Hit
TGTAAACATCCTACACTCTCAGCT106550.10359520574416213No Hit
TGAGGTAGTAAGTTGTATTGTT105950.10301184466066617No Hit
TCCTGTACTGAGCTGCCCCGAG103060.1002019887751605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)