FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7888638
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG111793714.171483087448047No Hit
CCCCCCACTGCTAAATTTGACTG97372812.343423541554321No Hit
CCCCCACTGCTAAATTTGACTG3231124.095916177165184No Hit
TCGTACGACTCTTAGCGG2848053.610319043667614No Hit
TTCAAGTAATCCAGGATAGGCT2210482.8021060162730245No Hit
AACATTCAACGCTGTCGGTGAG2061042.612669005726971No Hit
TAGCAGCACGTAAATATTGGCG1304781.6539990807031582No Hit
TGAGGTAGTAGATTGTATAGTT912481.156701575100797No Hit
TGAGGTAGTAGGTTGTATAGTT887871.1255048083078474No Hit
TATTGCACTTGTCCCGGCCTGTAA884371.1210680474880454No Hit
AAGCTGCCAGTTGAAGAACTGT860961.0913924558333137No Hit
TATTGCACTTGTCCCGGCCTGT817381.0361484453970382No Hit
TAGCTTATCAGACTGATGTTGAC790901.002581180680366No Hit
AACATTCAACGCTGTCGGTGAGT774650.9819819340170002No Hit
TATTGCACTTGTCCCGGCCTGTAT721670.9148220516646854No Hit
TAGCTTATCAGACTGATGTTGA660030.8366843553982323No Hit
TAGCTTATCAGACTGATGTTGACT568070.72011163397281No Hit
CAACGGAATCCCAAAAGCAGCTGT506980.6426711429780401No Hit
CCCCCACTGCTAAATTTGACTGGCTT499030.6325933576873474No Hit
TATTGCACTTGTCCCGGCCTGTA469480.5951344199087345No Hit
TGAGAACTGAATTCCATGGGTT419030.5311816818061622No Hit
TTAATGCTAATCGTGATAGGGGTT366240.46426265218406526No Hit
AGCAGCATTGTACAGGGCTATGA365850.46376827026414447No Hit
ACTGGACTTGGAGTCAGAAGGC359020.45511024843578834No Hit
CAACGGAATCCCAAAAGCAGCT333270.42246836526153186No Hit
GGAATACCGGGTGCTGTAGGCTTT332210.42112466055610615No Hit
TTCTCACTACTGCACTTGACTAG331660.42042745528442294No Hit
TGAGGTAGTAGTTTGTACAGTT319050.4044424398736512No Hit
CACCCGGCTGTGTGCACATGTGC317150.4020339125714731No Hit
TGTAAACATCCTTGACTGGAAGCT303340.3845277220224835No Hit
TTCAAGTAATTCAGGATAGGTT277920.35230416201123693No Hit
AACGGAATCCCAAAAGCAGCTG266630.33799243925250466No Hit
CTCCCACTGCTTCACTTGACTAG264620.3354444708959899No Hit
GGAATACCGGGTGCTGTAGGCTT263380.33387258991983154No Hit
TGTAAACATCCCCGACTGGAAGCT251900.3193200144308815No Hit
CATTGCACTTGTCTCGGTCTGA234710.2975291805759118No Hit
GCCTGTCTGAGCGTCGCT231270.29316847851302086No Hit
ACCCCCCACTGCTAAATTTGACTG210980.26744794221765533No Hit
CATAAAGTAGAAAGCACTACT196720.24937131099183407No Hit
ACCGGGTGCTGTAGGCTT183780.2329679724180524No Hit
TCGTTTCCCGGCCAATGCACCA183440.23253697279555738No Hit
CCCCCCACTGCTAAATTTGACTGG180220.22845515284133966No Hit
CTTCTCACTACTGCACTTGACTAG177400.2248803912665279No Hit
CCCCCCACTGCTAAATTTGACTGGCT170700.21638716341147862No Hit
CCCATAAAGTAGAAAGCACTAC168750.21391525381187476No Hit
CCCATAAAGTAGAAAGCACT156470.19834856156411285No Hit
TAGCAGCACGTAAATATTGGC155200.19673865120949904No Hit
CTCCCACTGCTTCACTTGACTAGCCT145000.18380866253464792No Hit
CACTAGATTGTGAGCTCCTGGA138770.17591122827540065No Hit
ACCGGGTGCTGTAGGCTTT133860.16968708666819293No Hit
AGCTCGGTCTGAGGCCCCTCAGT133210.16886311680165828No Hit
TAGCTTATCAGACTGATGTTGACA132250.16764617669108406No Hit
ACTGGACTTGGAGTCAGAAGGCT131170.16627711906668807No Hit
AATGCACCCGGGCAAGGATTCT129200.1637798565481139No Hit
CACTAGATTGTGAGCTCCTGGAA125920.1596219778369853No Hit
TATTGCACTTGTCCCGGCCTGTT125390.15895012548427245No Hit
CAAAGAATTCTCCTTTTGGGCT115370.146248313080154No Hit
GACCCGCGGGCGCTCTCCAGTCCT113080.14334540385805508No Hit
TGAGAACTGAATTCCATAGGCTGT111900.14184958163880762No Hit
ACTGGACTTGGAGTCAGAAGGCA109980.1394157014176592No Hit
ACCGGGTGCTGTAGGCTTA108090.13701985057496618No Hit
TGATGTAGTAGGTTGTATAGTT106510.13501696997631277No Hit
TGATGTAGTAGATTGTATAGTT106250.1346873820296989No Hit
TTCAAGTAATCCAGGATAGGC104580.1325704132956792No Hit
TTCACAGTGGCTAAGTTCCGC103020.13059288561599608No Hit
TATTGCACTTGTCCCGGCCTGTAG101900.12917312215365948No Hit
TGAGGTAGTAGATTGTATAGT99380.12597865436340214No Hit
TCCTGTACTGAGCTGCCCCGAGT98110.12436874400878833No Hit
GTCAGGATGGCCGAGCGGTCTAAGG97040.12301236284387748No Hit
TGTAAACATCCCCGACTGGA96360.12215036359888741No Hit
CCCATAAAGTAGAAAGCACTA92650.11744739712989745No Hit
TTCACAGTGGCTAAGTTCTGC92150.11681357415564006No Hit
CTCGTACGACTCTTAGCGG91930.1165346920469668No Hit
TGAGGTAGTAGTTTGTGCTGTT88220.11183172557797684No Hit
TCCTGTACTGAGCTGCCCCGAGA86890.11014575646645212No Hit
CAACACCAGTCGATGGGCTGT85020.10777525854272943No Hit
ATGCACCTGGGCAAGGATTCTGA83290.1055822310517988No Hit
CAAAGTGCTGTTCGTGCAGGTAG80100.10153844047603655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)