FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5649078
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT58716910.394067846115773No Hit
CAACGGAATCCCAAAAGCAGCTG2630064.655733201063962No Hit
AAGCTGCCAGTTGAAGAACTGT2170993.843087314425469No Hit
TCCTGTCTGAGCGTCGCT2049993.6288930689220433No Hit
TTCAAGTAATCCAGGATAGGCT1505032.6642046719836405No Hit
TAGCAGCACGTAAATATTGGCG1285912.2763183655810737No Hit
AACATTCAACGCTGTCGGTGAGT1252082.2164324868589174No Hit
CCCCCCACTGCTAAATTTGACTG1125051.991563933087842No Hit
CGCCTGTCTGAGCGTCGCT888931.5735842202922319No Hit
TAGCTTATCAGACTGATGTTGAC693711.2280057028775315No Hit
AACATTCAACGCTGTCGGTGAG622951.1027463242674291No Hit
TATTGCACTTGTCCCGGCCTGT617051.0923021420486672No Hit
TGAGGTAGTAGGTTGTATAGTT605051.0710597375359308No Hit
CATAAAGTAGAAAGCACTACT592221.0483480667110632No Hit
TAGCTTATCAGACTGATGTTGA571121.0109968387761685No Hit
CCCCCACTGCTAAATTTGACTG543290.9617321623103806No Hit
TAGCTTATCAGACTGATGTTGACT382220.676605987738176No Hit
TGAGGTAGTAGATTGTATAGTT349690.6190213695048997No Hit
AGCAGCATTGTACAGGGCTATGA315000.5576131184593309No Hit
TGTAAACATCCTTGACTGGAAGCT313500.5549578178952388No Hit
TTCAAGTAATTCAGGATAGGTT305890.5414865930334118No Hit
ACCGGGTGCTGTAGGCTT297770.5271125659797935No Hit
TCGTACGACTCTTAGCGG288130.5100478343545619No Hit
TATTGCACTTGTCCCGGCCTGTAA242860.4299108633302638No Hit
TATTGCACTTGTCCCGGCCTGTAT234840.4157138563142516No Hit
CCCATAAAGTAGAAAGCACTAC233420.4132001717802445No Hit
CATTGCACTTGTCTCGGTCTGA206060.36476748949120547No Hit
TATTGCACTTGTCCCGGCCTGTA194900.3450120532943606No Hit
ACGCCTGTCTGAGCGTCGCT189520.33548837527115044No Hit
ACTGGACTTGGAGTCAGAAGGC185240.32791191766160777No Hit
ATCCCGGACGAGCCCCCA179170.3171668013789153No Hit
CTACGCCTGTCTGAGCGTCGCT174520.30893536963022994No Hit
CAACACCAGTCGATGGGCTGT173760.30759001734442326No Hit
CCCTGTCTGAGCGTCGCT172360.30511173681793735No Hit
TGATGTAGTAGGTTGTATAGTT170820.30238562823880283No Hit
TGTAAACATCCCCGACTGGAAGCT168090.29755298121215534No Hit
GGAATACCGGGTGCTGTAGGCTTT166000.2938532624261871No Hit
CAACGGAATCCCAAAAGCAGCTGT161970.28671935491065975No Hit
TACGCCTGTCTGAGCGTCGCT161930.2866485468956173No Hit
TGAGGTAGTAGTTTGTACAGTT161290.285515618654938No Hit
CCCATAAAGTAGAAAGCACT153270.2713186116389259No Hit
GGAATACCGGGTGCTGTAGGCTT149930.2654061423828809No Hit
ACCGGGTGCTGTAGGCTTT148100.2621666756946886No Hit
ATCCCACCGCTGCCACCA145950.2583607448861566No Hit
TACCTGGTTGATCCTGCC132870.23520652396727396No Hit
TTCTCACTACTGCACTTGACTAG127640.225948376000473No Hit
GCGTTCGCGCTTTCCCCTG127290.2253288058688515No Hit
CTCCCACTGCTTCACTTGACTAG121330.21477841162752576No Hit
TGAGGTAGTAGTTTGTGCTGTT119440.21143273291676978No Hit
TGTAAACATCCTACACTCTCAGCT112920.19989102646484966No Hit
AACCGGGCGGAAACACCA106330.18822540598660525No Hit
AACATTCAACGCTGTCGGTGAGTT104810.18553470141499198No Hit
TTATAAAGCAATGAGACTGATT101690.18001167624168052No Hit
TTCACAGTGGCTAAGTTCCG100090.17717935563998233No Hit
TGATGTAGTAGATTGTATAGTT99450.17604642739930304No Hit
AGCTCGGTCTGAGGCCCCTCAGT92100.163035454635252No Hit
ACCTGTCTGAGCGTCGCT91810.1625220965261942No Hit
ACCGGGTGCTGTAGGCTTA84970.15041392595393444No Hit
TTCACAGTGGCTAAGTTCCGC84030.14874993760043673No Hit
CAACGGAATCCCAAAAGCAGCT82300.14568749094985056No Hit
TTCAAGTAATCCAGGATAGGCTT80400.14232411023533398No Hit
AGCAGCATTGTACAGGGCTATG79240.1402706777991028No Hit
TAGCAGCACGTAAATATTGGC79180.1401644657765391No Hit
TTCAAGTAATCCAGGATAGGC77810.13773929126133505No Hit
GCCTGTCTGAGCGTCGCA77210.1366771710356982No Hit
TATTGCACTTGTCCCGGCCTGTT76140.13478305663331255No Hit
TGTAAACATCCTACACTCAGCT75700.13400416846784555No Hit
GCCGGTCTGAGCGTCGCT75260.13322528030237854No Hit
TAGCAGCACGTAAATATTGGCGT73370.12987960159162257No Hit
ACCACAGGGTAGAACCACGGAC71630.1267994529372758No Hit
TGAGGTAGTAGGTTGTATAGT71180.12600286276804817No Hit
CAAAGTGCTGTTCGTGCAGGT71030.12573733271163895No Hit
TGTCAGTTTGTCAAATACCCCA70880.12547180265522975No Hit
CCTCGACACAAGGGTTTGT68840.12186059388806457No Hit
TAGCTTATCAGACTGATGTTGACA67230.11901057128260577No Hit
CTTCTCACTACTGCACTTGACTAG67070.11872733922243595No Hit
ACCTGGTGCTGTAGGCTT66940.11849721317354796No Hit
TGAATACCGGGTGCTGTAGGCTTT66610.11791304704944772No Hit
TACCACCCTGAACGCGCCCGA65990.11681552281628967No Hit
TTCACAGTGGCTAAGTTCTGC65480.11591272062449838No Hit
AGCAGCATTGTACAGGGCTATGT65260.11552327654176488No Hit
TGAGGTAGTAGGTTGTGTGGTT65160.11534625650415874No Hit
TGAATACCGGGTGCTGTAGGCTT63050.11161113371066925No Hit
TCCGGTCTGAGCGTCGCT62850.11125709363545697No Hit
TCGTTTCCCGGCCAATGCACCA62340.11035429144366567No Hit
AACGGAATCCCAAAAGCAGCTG60890.10778750089837669No Hit
CGCGACCTCAGATCAGACGTGG60880.10776979889461608No Hit
GCCTGTCTGAGCGTCGCG60860.10773439488709485No Hit
CACCCGGCTGTGTGCACATGTGC60330.10679618868778232No Hit
CCCCCACTGCTAAATTTGACTGGCTT60130.10644214861257005No Hit
TAGCAGCACATAATGGTTTGT60020.1062474265712033No Hit
AGAGGTAGTAGGTTGCATAGTT59300.10497288230043912No Hit
TGTCAGTTTGTCAAATACCCCAA58110.10286634385292609No Hit
CCCCCCACTGCTAAATTTGACTGG58110.10286634385292609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)