FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8326299
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6363097.642158899169968No Hit
CCCCCCACTGCTAAATTTGACTG5117576.1462721912821054No Hit
AACATTCAACGCTGTCGGTGAGT2424992.9124464543010045No Hit
GCCTGTCTGAGCGTCGCT2413992.8992353025035493No Hit
TTCAAGTAATCCAGGATAGGCT2395052.876488101135931No Hit
AAGCTGCCAGTTGAAGAACTGT1910642.294705006389994No Hit
AACATTCAACGCTGTCGGTGAG1884672.2635146780100017No Hit
TAGCAGCACGTAAATATTGGCG1881582.2598035453687166No Hit
CCCCCACTGCTAAATTTGACTG1720012.065755745740094No Hit
TATTGCACTTGTCCCGGCCTGTAT1432491.7204402580306088No Hit
TATTGCACTTGTCCCGGCCTGTAA1229231.4763221930896309No Hit
TATTGCACTTGTCCCGGCCTGT1150931.3822828125677447No Hit
TGAGGTAGTAGGTTGTATAGTT1077331.2938881969047713No Hit
TAGCTTATCAGACTGATGTTGACT927741.1142285425973775No Hit
TAGCTTATCAGACTGATGTTGAC918261.1028429317755706No Hit
TCCTGTCTGAGCGTCGCT883581.0611917731995932No Hit
GGAATACCGGGTGCTGTAGGCTTT876121.052232210253319No Hit
TAGCTTATCAGACTGATGTTGA751110.9020934751442387No Hit
TATTGCACTTGTCCCGGCCTGTA685640.8234631016733847No Hit
GGAATACCGGGTGCTGTAGGCTT679920.8165933027387077No Hit
TGAGGTAGTAGATTGTATAGTT644200.7736930898109712No Hit
TTCAAGTAATTCAGGATAGGTT574750.6902826814170377No Hit
TGTAAACATCCTTGACTGGAAGCT525830.6315290863323548No Hit
AGCAGCATTGTACAGGGCTATGA479690.5761143096110288No Hit
CATAAAGTAGAAAGCACTACT438630.5268006829925277No Hit
TGTAAACATCCCCGACTGGAAGCT409740.49210339431721106No Hit
TTAATGCTAATCGTGATAGGGGTT407040.48886065705783566No Hit
TGAATACCGGGTGCTGTAGGCTTT370620.4451197344702611No Hit
TCGTACGACTCTTAGCGG364060.4372410839437786No Hit
ACTGGACTTGGAGTCAGAAGGC345540.4149983083720631No Hit
CAACGGAATCCCAAAAGCAGCTGT342710.4115994393187177No Hit
CTCCCACTGCTTCACTTGACTAG315130.37847547872109805No Hit
CATTGCACTTGTCTCGGTCTGA293990.3530860469939886No Hit
TGATGTAGTAGGTTGTATAGTT293850.3529179050620209No Hit
TGAATACCGGGTGCTGTAGGCTT284050.3411479698242881No Hit
ATCCCGGACGAGCCCCCA274860.33011065300441406No Hit
ACCGGGTGCTGTAGGCTT260040.3123116284918425No Hit
TGAGAACTGAATTCCATGGGTT257300.3090208506804764No Hit
CCCATAAAGTAGAAAGCACTAC255530.30689505625488583No Hit
CACCCGGCTGTGTGCACATGTGC233220.280100438382047No Hit
ACCGGGTGCTGTAGGCTTA233140.28000435727806555No Hit
AACCGGGCGGAAACACCA229780.2759689509108429No Hit
TGAGGTAGTAGTTTGTACAGTT217990.26180899821157033No Hit
TTCTCACTACTGCACTTGACTAG216540.2600675282019058No Hit
TATTGCACTTGTCCCGGCCTGTT213080.25591202045470623No Hit
CCCCCACTGCTAAATTTGACTGGCTT204510.2456193321906888No Hit
CACTAGATTGTGAGCTCCTGGA200990.24139176361550313No Hit
CCCATAAAGTAGAAAGCACT196960.23655167800243543No Hit
AGCTCGGTCTGAGGCCCCTCAGT195590.23490628909675235No Hit
CAACGGAATCCCAAAAGCAGCT192050.2306547002455713No Hit
CAACACCAGTCGATGGGCTGT182640.21935316038974817No Hit
TGATGTAGTAGATTGTATAGTT175840.2111862665513213No Hit
ACCGGGTGCTGTAGGCTTT175230.21045364813346243No Hit
CACTAGATTGTGAGCTCCTGGAA170910.2052652685184618No Hit
TATTGCACTTGTCCCGGCCTGTAG168800.20273112940094995No Hit
TGTAAACATCCTACACTCTCAGCT160570.19284678582885384No Hit
AACATTCAACGCTGTCGGTGAGTT159460.19151366051111063No Hit
CGCCTGTCTGAGCGTCGCT159080.19105727526719854No Hit
ACCCCCCACTGCTAAATTTGACTG157630.189315805257534No Hit
TATTGCACTTGTCCCGGCCTGTTT148290.1780983363676947No Hit
GGAATACCGGGTGCTGTAGGCTTA145030.17418303138044886No Hit
CTTCTCACTACTGCACTTGACTAG137870.16558377257410525No Hit
TGAGGTAGTAGTTTGTGCTGTT136530.16397441408241525No Hit
GGAATACCGGGTGCTGTAGGCT134920.1620407818647877No Hit
AACGGAATCCCAAAAGCAGCTG134370.16138022427491494No Hit
TAGCTTATCAGACTGATGTTGACA132380.1589902068133753No Hit
CCCCCCACTGCTAAATTTGACTGGCT132110.15866593308743776No Hit
TACCACCCTGAACGCGCCCGA130800.15709260500974082No Hit
TTCAAGTAATCCAGGATAGGCTT130160.15632395617788888No Hit
CGCGACCTCAGATCAGACGTGGCG129510.15554329720803925No Hit
TAGCAGCACGTAAATATTGGC128400.15421017189029604No Hit
CCCCCCACTGCTAAATTTGACTGG127260.15284101615855974No Hit
CTCCCACTGCTTCACTTGACTAGCCT125640.15089537380293452No Hit
CAAAGAATTCTCCTTTTGGGCT122350.14694403840169565No Hit
CGCGACCTCAGATCAGACGTGG118420.14222405416860479No Hit
TGAGAACTGAATTCCATAGGCTGT117780.14145540533675285No Hit
TTCAAGTAATCCAGGATAGGC115350.13853694180331502No Hit
TTATAAAGCAATGAGACTGATT115230.13839282014734278No Hit
AGCAGCATTGTACAGGGCTATGT110300.13247182211448327No Hit
CCCCCCACTGCTAAATTTGACTGT109770.13183528480060588No Hit
TGTAAACATCCCCGACTGGAAGC109550.1315710617646568No Hit
TCGTTTCCCGGGCGGCGCACCA99710.11975308597493317No Hit
TATTGCACTTGTCCCGGCCTGA98360.11813171734524547No Hit
ACTTGACTTGGAGTCAGAAGGC96910.1163902473355809No Hit
TATTGCACTTGTCCCGGCCTGTAAT96420.11580175057369427No Hit
TGAGGTAGTAGGTTGTATAGT94410.11338771283615925No Hit
CCCCCCACTGCTAAATTTGACTGTA89970.1080552115651864No Hit
TGAGGTAGTAGGTTGTGTGGTT89330.10728656273333446No Hit
TATTGCACTTGTCCCGGCCTGTAAA87530.10512473789375087No Hit
TACCACCCTGAACGCGCCCGAT84470.10144963566645877No Hit
CAAAGAATTCTCCTTTTGGGCTT83770.1006089260066207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)