FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14579782
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT8989926.166018120161193No Hit
CAACGGAATCCCAAAAGCAGCTG7343065.036467623452806No Hit
CCCCCCACTGCTAAATTTGACTG5902614.048489888257588No Hit
AAGCTGCCAGTTGAAGAACTGT5092083.492562508822148No Hit
AACATTCAACGCTGTCGGTGAGT4675453.2068037779988754No Hit
TAGCAGCACGTAAATATTGGCG3920772.689182869812457No Hit
TTCAAGTAATCCAGGATAGGCT3799402.6059374550319063No Hit
TCCTGTCTGAGCGTCGCT3185052.184566271292671No Hit
CCCCCACTGCTAAATTTGACTG2268121.5556611203103037No Hit
AACATTCAACGCTGTCGGTGAG2142371.4694115453852465No Hit
TAGCTTATCAGACTGATGTTGAC2135391.4646240938307582No Hit
TAGCTTATCAGACTGATGTTGACT1801611.2356906296678511No Hit
TAGCTTATCAGACTGATGTTGA1654921.135078700079329No Hit
TATTGCACTTGTCCCGGCCTGT1583471.0860724803704198No Hit
TGAGGTAGTAGGTTGTATAGTT1453400.9968598981795476No Hit
TATTGCACTTGTCCCGGCCTGTAA1275590.8749033421761724No Hit
CGCCTGTCTGAGCGTCGCT1186430.8137501644400444No Hit
TATTGCACTTGTCCCGGCCTGTAT1153360.7910680694677054No Hit
TGTAAACATCCTTGACTGGAAGCT1013540.6951681444894031No Hit
CATAAAGTAGAAAGCACTACT1006100.6900651875316106No Hit
AGCAGCATTGTACAGGGCTATGA856300.5873201670642264No Hit
AACCGGGCGGAAACACCA826510.5668877627937098No Hit
TTAATGCTAATCGTGATAGGGGTT820800.5629713805048663No Hit
TGAGGTAGTAGATTGTATAGTT768300.5269626116494746No Hit
TCGTACGACTCTTAGCGG687670.4716600015007083No Hit
TATTGCACTTGTCCCGGCCTGTA683960.4691153818349273No Hit
TTCAAGTAATTCAGGATAGGTT678230.46518528191985314No Hit
ACTGGACTTGGAGTCAGAAGGC665200.45624824843060063No Hit
TGTAAACATCCCCGACTGGAAGCT581560.3988811355341253No Hit
GGAATACCGGGTGCTGTAGGCTTT559690.38388091125093643No Hit
ACCGGGTGCTGTAGGCTT529950.3634828010459964No Hit
CAACGGAATCCCAAAAGCAGCTGT480610.3296414171350436No Hit
CCCATAAAGTAGAAAGCACTAC471110.32312554467549653No Hit
GGAATACCGGGTGCTGTAGGCTT430080.2949838344633685No Hit
CATTGCACTTGTCTCGGTCTGA414070.2840028746657529No Hit
AACATTCAACGCTGTCGGTGAGTT384620.26380367004115696No Hit
TGTAAACATCCTACACTCTCAGCT359060.24627254371841775No Hit
CCCATAAAGTAGAAAGCACT347340.23823401474727127No Hit
TTCTCACTACTGCACTTGACTAG343280.2354493366224543No Hit
CTCCCACTGCTTCACTTGACTAG325590.22331609622146614No Hit
TACGCCTGTCTGAGCGTCGCT323720.22203349816890267No Hit
CAACACCAGTCGATGGGCTGT310590.21302787654849709No Hit
ACCGGGTGCTGTAGGCTTT305210.20933783509245887No Hit
CAACGGAATCCCAAAAGCAGCT296380.20328150311163776No Hit
TGATGTAGTAGGTTGTATAGTT292160.20038708397697577No Hit
TGAGGTAGTAGTTTGTACAGTT291630.20002356688186423No Hit
CTACGCCTGTCTGAGCGTCGCT275800.1891660657203242No Hit
TTCAAGTAATCCAGGATAGGCTT275740.18912491284163233No Hit
GCGTTCGCGCTTTCCCCTG273220.18739649193657354No Hit
ACGCCTGTCTGAGCGTCGCT272910.18718386872999884No Hit
CTTCTCACTACTGCACTTGACTAG255000.1748997344404738No Hit
ACCGGGTGCTGTAGGCTTA248780.17063355268274932No Hit
TTATAAAGCAATGAGACTGATT246220.16887769652522924No Hit
TAGCTTATCAGACTGATGTTGACA242230.16614103009221948No Hit
AGCTCGGTCTGAGGCCCCTCAGT237550.16293110555425314No Hit
TATTGCACTTGTCCCGGCCTGTT232990.15980348677367057No Hit
CACCCGGCTGTGTGCACATGTGC224740.15414496595353758No Hit
CCCCCACTGCTAAATTTGACTGGCTT224270.1538226017371179No Hit
TGAGGTAGTAGTTTGTGCTGTT219690.15068126533030465No Hit
CGCGACCTCAGATCAGACGTGGCG208510.14301311226738508No Hit
TGAATACCGGGTGCTGTAGGCTTT206030.14131212661478754No Hit
CCCTGTCTGAGCGTCGCT194090.13312270375510415No Hit
TCCGGTCTGAGCGTCGCT192130.13177837638450288No Hit
TAGCAGCACGTAAATATTGGC184330.12642850215455897No Hit
ATCCCGGACGAGCCCCCA181020.12415823501339047No Hit
AGCAGCATTGTACAGGGCTATGT179110.12284820170836573No Hit
ACCACAGGGTAGAACCACGGAC178950.12273846069852073No Hit
TTCACAGTGGCTAAGTTCCG175790.12057107575408192No Hit
CCCCCCACTGCTAAATTTGACTGG174750.1198577591900894No Hit
CAAAGAATTCTCCTTTTGGGCT171210.1174297393472687No Hit
TTCAAGTAATCCAGGATAGGC169320.1161334236684746No Hit
CAAAGTGCTGTTCGTGCAGGT167740.11504973119625521No Hit
CGCGACCTCAGATCAGACGTGG166600.11426782650110956No Hit
TGTAAACATCCCCGACTGGAAGC160490.11007709168765348No Hit
AGCAGCATTGTACAGGGCTATG159230.10921288123512408No Hit
CAAAGAATTCTCCTTTTGGGCTT158950.10902083446789534No Hit
TGAATACCGGGTGCTGTAGGCTT158860.10895910514985752No Hit
TGATGTAGTAGATTGTATAGTT157900.1083006590907875No Hit
AACGGAATCCCAAAAGCAGCTG157030.10770394234975529No Hit
CCCCCCACTGCTAAATTTGACTGGCT155890.10692203765460964No Hit
TCGTTTCCCGGCCAATGCACCA155800.10686030833657184No Hit
ACTTGACTTGGAGTCAGAAGGC154980.1062978856611162No Hit
CACTAGATTGTGAGCTCCTGGA154970.10629102684800089No Hit
TGAGGTAGTAGGTTGTGTGGTT151740.10407563021175488No Hit
TGTAAACATCCTACACTCAGCT148660.10196311577223857No Hit
TCCTTCTGATCGAGGCCC147360.10107147006724793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)