FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7726821
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4769586.172758499258622No Hit
CCCCCCACTGCTAAATTTGACTG4157075.3800521585785415No Hit
AACATTCAACGCTGTCGGTGAGT3141284.065423542230369No Hit
AAGCTGCCAGTTGAAGAACTGT2850773.689447445463018No Hit
TAGCAGCACGTAAATATTGGCG2459753.1833919797029075No Hit
TTCAAGTAATCCAGGATAGGCT2238262.896741104782937No Hit
CCCCCACTGCTAAATTTGACTG1481811.9177485799140421No Hit
AACATTCAACGCTGTCGGTGAG1406581.8203864176483446No Hit
TAGCTTATCAGACTGATGTTGAC1086661.406348095808095No Hit
TATTGCACTTGTCCCGGCCTGT925031.1971676320701619No Hit
TAGCTTATCAGACTGATGTTGA909351.1768746810622377No Hit
TAGCTTATCAGACTGATGTTGACT827631.0711132042530815No Hit
TATTGCACTTGTCCCGGCCTGTAA710990.9201584972655638No Hit
TGAGGTAGTAGGTTGTATAGTT682880.8837787234879648No Hit
TATTGCACTTGTCCCGGCCTGTAT663080.8581536960672441No Hit
CATAAAGTAGAAAGCACTACT577990.7480307878233493No Hit
TTAATGCTAATCGTGATAGGGGTT548200.7094767692949016No Hit
TGTAAACATCCTTGACTGGAAGCT483530.6257812883202549No Hit
TATTGCACTTGTCCCGGCCTGTA462120.5980726096799706No Hit
CGCCTGTCTGAGCGTCGCT456670.5910192561727521No Hit
AGCAGCATTGTACAGGGCTATGA437750.566533118859619No Hit
ACTGGACTTGGAGTCAGAAGGC417890.5408304398406538No Hit
TCGTACGACTCTTAGCGG390720.5056672077688871No Hit
TTCAAGTAATTCAGGATAGGTT378960.490447494512944No Hit
ACCGGGTGCTGTAGGCTT373340.48317412814403227No Hit
TGAGGTAGTAGATTGTATAGTT350820.4540288949362228No Hit
TGTAAACATCCCCGACTGGAAGCT337770.43713967231802053No Hit
CAACGGAATCCCAAAAGCAGCTGT293610.3799880960099891No Hit
AACATTCAACGCTGTCGGTGAGTT290560.3760408064325549No Hit
AACCGGGCGGAAACACCA289490.3746560195971927No Hit
CATTGCACTTGTCTCGGTCTGA276230.35749501638513437No Hit
CCCATAAAGTAGAAAGCACTAC274310.3550101652413069No Hit
GGAATACCGGGTGCTGTAGGCTTT248710.32187881665694085No Hit
TGTAAACATCCTACACTCTCAGCT221890.28716855224160104No Hit
ACCGGGTGCTGTAGGCTTT220910.28590024280360576No Hit
GGAATACCGGGTGCTGTAGGCTT210770.27277712270026705No Hit
CCCATAAAGTAGAAAGCACT207320.2683121558012021No Hit
GCCTGTCTGAGCGTCGCT201970.2613882216243912No Hit
TTCTCACTACTGCACTTGACTAG194390.25157823637948906No Hit
CTACGCCTGTCTGAGCGTCGCT188370.24378719268894675No Hit
CAACACCAGTCGATGGGCTGT173660.224749609185977No Hit
TACGCCTGTCTGAGCGTCGCT168420.21796803627261457No Hit
TTCACAGTGGCTAAGTTCCG162910.2108370311671514No Hit
CTTCTCACTACTGCACTTGACTAG162380.21015110871599071No Hit
CTCCCACTGCTTCACTTGACTAG160600.207847444634734No Hit
ACCGGGTGCTGTAGGCTTA155150.20079409112751545No Hit
ATCCCGGACGAGCCCCCA151670.19629029842932819No Hit
CAACGGAATCCCAAAAGCAGCT150750.19509964058957752No Hit
TGAGGTAGTAGTTTGTACAGTT148930.19274420877615775No Hit
TATTGCACTTGTCCCGGCCTGTT146640.18978050610982186No Hit
TGATGTAGTAGGTTGTATAGTT146390.18945695778380267No Hit
ACGCCTGTCTGAGCGTCGCT142670.184642558692637No Hit
TGAATACCGGGTGCTGTAGGCTTT141870.18360720404937556No Hit
CACTAGATTGTGAGCTCCTGGA139860.18100587550818117No Hit
CCCCCACTGCTAAATTTGACTGGCTT138150.17879280495820984No Hit
TTCAAGTAATCCAGGATAGGCTT133320.1725418512995189No Hit
CCCCCCACTGCTAAATTTGACTGG129240.16726154261888557No Hit
TGAGGTAGTAGTTTGTGCTGTT128280.16601911704697184No Hit
GCGTTCGCGCTTTCCCCTG124790.16150238241574383No Hit
TGAATACCGGGTGCTGTAGGCTT124590.16124354375492844No Hit
AGCTCGGTCTGAGGCCCCTCAGT123060.15926342799969095No Hit
TTATAAAGCAATGAGACTGATT118010.1527277518141031No Hit
CAAAGAATTCTCCTTTTGGGCT115950.1500617136077049No Hit
ACTGGACTTGGAGTCAGAAGG111550.144367263069767No Hit
TAGCTTATCAGACTGATGTTGACA111330.1440825405428701No Hit
CCCCCCACTGCTAAATTTGACTGGCT109220.14135179267126805No Hit
ACCACAGGGTAGAACCACGGAC106230.13748215469207842No Hit
TGTAAACATCCTACACTCAGCT105580.1366409290444285No Hit
CACCCGGCTGTGTGCACATGTGC105410.13642091618273544No Hit
TTCAAGTAATCCAGGATAGGC104940.13581264532981932No Hit
TCCTGTCTGAGCGTCGCT104610.13538556153947398No Hit
AACGGAATCCCAAAAGCAGCTG104370.13507495514649556No Hit
CGCGACCTCAGATCAGACGTGGCG104080.1346996390883133No Hit
ACTGGACTTGGAGTCAGAAGGCA103510.13396194890498953No Hit
TCGTTTCCCGGCCAATGCACCA100870.13054527858222675No Hit
CGCGACCTCAGATCAGACGTGG100620.13022173025620756No Hit
TAGCAGCACGTAAATATTGGC100610.1302087883231668No Hit
AACATTCAACGCTGTCGGTGA98760.1278145307106247No Hit
AGCAGCATTGTACAGGGCTATG97580.1262873826118141No Hit
CAAAGAATTCTCCTTTTGGGCTT97370.12601560201795797No Hit
TGTAAACATCCCCGACTGGAAGC97260.1258732407545095No Hit
ACTTGACTTGGAGTCAGAAGGC94410.12218478983789065No Hit
AGCAGCATTGTACAGGGCTATGT90420.11702095855462422No Hit
TATTGCACTTGTCCCGGCCTGTAG87400.11311249477631229No Hit
CACTAGATTGTGAGCTCCTGGAA86810.11234892072690696No Hit
TGCAAGTAATCCAGGATAGGCT85720.11093825002546326No Hit
TAGCAGCACGTAAATATTGGCGT85410.11053705010119945No Hit
CAAAGTGCTGTTCGTGCAGGT83410.10794866349304584No Hit
ACCCCCCACTGCTAAATTTGACTG83280.10778041836351586No Hit
ACTGGACTTGGAGTCAGAAGGCT81040.10488142536238385No Hit
TTCACAGTGGCTAAGTTCTG78460.10154240663786569No Hit
AACATTCAACGCTGTCGGTGAGA78100.10107649704839805No Hit
CCCATAAAGTAGAAAGCACTA77910.10083060032062346No Hit
TGTCAGTTTGTCAAATACCCCA77670.10051999392764503No Hit
AGCTACATCTGGCTACTGGGTCTC77330.10007996820425889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)