FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7927511
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7417909.357161409173699No Hit
CCCCCCACTGCTAAATTTGACTG7128898.99259553219163No Hit
AACATTCAACGCTGTCGGTGAGT2984183.7643341018385215No Hit
TTCAAGTAATCCAGGATAGGCT2484313.133783100395572No Hit
TAGCAGCACGTAAATATTGGCG2397483.024253135694167No Hit
CCCCCACTGCTAAATTTGACTG2280642.8768676574526353No Hit
AACATTCAACGCTGTCGGTGAG1996162.5180160582558635No Hit
AAGCTGCCAGTTGAAGAACTGT1987942.5076471038639996No Hit
TATTGCACTTGTCCCGGCCTGTAA1128341.4233218976296595No Hit
TAGCTTATCAGACTGATGTTGACT1000591.262174218364377No Hit
TATTGCACTTGTCCCGGCCTGTAT962311.2138866789336527No Hit
TAGCTTATCAGACTGATGTTGAC955201.2049179118136828No Hit
TAGCTTATCAGACTGATGTTGA829911.0468733502861114No Hit
TGAGGTAGTAGGTTGTATAGTT811301.0233981384573292No Hit
TATTGCACTTGTCCCGGCCTGT742000.9359810412120525No Hit
GGAATACCGGGTGCTGTAGGCTTT519510.6553254861456516No Hit
TGTAAACATCCTTGACTGGAAGCT504200.6360129932333112No Hit
TGAGGTAGTAGATTGTATAGTT483780.6102545931503596No Hit
TTCAAGTAATTCAGGATAGGTT466240.5881291113944843No Hit
TATTGCACTTGTCCCGGCCTGTA463950.5852404367524687No Hit
CATAAAGTAGAAAGCACTACT420490.5304186900529057No Hit
CCCCCACTGCTAAATTTGACTGGCTT393400.49624655203884294No Hit
TGTAAACATCCCCGACTGGAAGCT389690.4915666468327827No Hit
GGAATACCGGGTGCTGTAGGCTT384740.4853225684581201No Hit
CAACGGAATCCCAAAAGCAGCTGT380630.4801380912621881No Hit
AGCAGCATTGTACAGGGCTATGA346270.43679535733220676No Hit
ACTGGACTTGGAGTCAGAAGGC334010.4213302258426384No Hit
TTCTCACTACTGCACTTGACTAG330020.4162971202436679No Hit
CATTGCACTTGTCTCGGTCTGA239530.3021503218349366No Hit
CTTCTCACTACTGCACTTGACTAG238480.3008258203615233No Hit
CCCATAAAGTAGAAAGCACTAC236220.29797498861874805No Hit
CAACGGAATCCCAAAAGCAGCT231110.2915290814481367No Hit
CTCCCACTGCTTCACTTGACTAG230930.2913020240526945No Hit
TGAATACCGGGTGCTGTAGGCTTT223410.281816070643106No Hit
CACCCGGCTGTGTGCACATGTGC210350.26534179517379414No Hit
AACATTCAACGCTGTCGGTGAGTT205740.2595266029905225No Hit
CCCCCCACTGCTAAATTTGACTGGCT195570.24669786014803385No Hit
ACCCCCCACTGCTAAATTTGACTG187370.2363541343556635No Hit
CTCCCACTGCTTCACTTGACTAGCCT180690.22792778212480563No Hit
TGAGGTAGTAGTTTGTACAGTT177500.2239038205055786No Hit
CAAAGAATTCTCCTTTTGGGCT171120.21585589726712456No Hit
CCCCCCACTGCTAAATTTGACTGG170630.2152377965795317No Hit
TGAATACCGGGTGCTGTAGGCTT169360.21363578051168897No Hit
CCCATAAAGTAGAAAGCACT167300.21103723476384958No Hit
TAGCAGCACGTAAATATTGGC166310.20978841908891707No Hit
ACCGGGTGCTGTAGGCTT154550.19495400258668832No Hit
CAACACCAGTCGATGGGCTGT151330.19089219806822091No Hit
TAGCTTATCAGACTGATGTTGACA148060.18676732205101954No Hit
AACGGAATCCCAAAAGCAGCTG140150.17678941095130615No Hit
TCGTACGACTCTTAGCGG137930.1739890364075181No Hit
TCGTTTCCCGGCCAATGCACCA137440.1733709357199252No Hit
TGATGTAGTAGGTTGTATAGTT136820.17258884913562403No Hit
TATTGCACTTGTCCCGGCCTGTT135870.17139049065967868No Hit
TGAGGTAGTAGTTTGTGCTGTT125400.15818331882478623No Hit
TGTAAACATCCTACACTCTCAGCT124890.1575399895376998No Hit
TATTGCACTTGTCCCGGCCTGTAG123980.1563920882607416No Hit
TGTAAACATCCCCGACTGGAAGC123830.1562028737645397No Hit
TTCAAGTAATCCAGGATAGGCTT119370.15057689607746996No Hit
TGTCAGTTTGTCAAATACCCCAA117930.14876043691393173No Hit
CACTAGATTGTGAGCTCCTGGA115820.1460988196673584No Hit
CAAAGAATTCTCCTTTTGGGCTT115160.14526627588407004No Hit
TTCAAGTAATCCAGGATAGGC110820.13979166979396182No Hit
GCCTGTCTGAGCGTCGCT108220.1365119518597956No Hit
ACTGGACTTGGAGTCAGAAGGCT106550.13440536380208112No Hit
TTCACAGTGGCTAAGTTCCG102720.12957408699905937No Hit
CACTAGATTGTGAGCTCCTGGAA102130.12882984331399855No Hit
ACCGGGTGCTGTAGGCTTT102120.12881722901425177No Hit
ACCGGGTGCTGTAGGCTTA101890.1285271001200755No Hit
AACCGGGCGGAAACACCA97470.12295157963199295No Hit
TATTGCACTTGTCCCGGCCTGTAAA93180.11754004504061868No Hit
TTCACAGTGGCTAAGTTCTGC91810.11581188597530802No Hit
TGTCAGTTTGTCAAATACCCCA91750.11573620017682724No Hit
CGCCTGTCTGAGCGTCGCT91090.1149036563935389No Hit
CTACGCCTGTCTGAGCGTCGCT88720.11191406735354893No Hit
AGCTCGGTCTGAGGCCCCTCAGT88450.11157348126038551No Hit
AACATTCAACGCTGTCGGTGA87670.11058956588013566No Hit
CCCCCCACTGCTAAATTTGACTGTA86520.10913892140925444No Hit
CGCCCCACTGCTAAATTTGACTG86080.10858389222039554No Hit
TATTGCACTTGTCCCGGCCTGTAAT85890.10834422052520647No Hit
ACTGGACTTGGAGTCAGAAGGCA85870.10831899192571287No Hit
TATTGCACTTGTCCCGGCCTGTTT84820.1069944904522996No Hit
TTCACAGTGGCTAAGTTCCGC84020.10598534647255614No Hit
TTCACAGTGGCTAAGTTCTG83680.10555646028116517No Hit
CGCGACCTCAGATCAGACGTGGCG83510.10534201718546969No Hit
CAACGGAATCCCAAAAGCAGCTGA83120.10485005949534476No Hit
ACTTGACTTGGAGTCAGAAGGC82300.10381568691610772No Hit
AATGCACCCGGGCAAGGATTCT81940.10336157212522316No Hit
TGATGTAGTAGATTGTATAGTT80460.10149465576269777No Hit
CCCCCCACTGCTAAATTTGACTGT80420.1014441985637106No Hit
GGAATACCGGGTGCTGTAGGCTTA79380.10013231139004411No Hit
CAAAGTGCTGTTCGTGCAGGT79370.10011969709029732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)