FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171700
Sequences flagged as poor quality0
Sequence length18-26
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT275054933.65944662677289No Hit
CAACGGAATCCCAAAAGCAGCTG4663015.70629122459219No Hit
CCCCCCACTGCTAAATTTGACTG2923263.5772972576085764No Hit
TTCAAGTAATCCAGGATAGGCT2306542.822595053660805No Hit
TCCTGTCTGAGCGTCGCT1979632.4225436567666456No Hit
TAGCAGCACGTAAATATTGGCG1833552.24378036393896No Hit
AAGCTGCCAGTTGAAGAACTGT1551581.8987236437950485No Hit
AACATTCAACGCTGTCGGTGAGT1333351.6316678292154632No Hit
AACATTCAACGCTGTCGGTGAG1126641.3787094484623763No Hit
TGAGGTAGTAGGTTGTATAGTT1075931.3166538174431268No Hit
CCCCCACTGCTAAATTTGACTG959621.1743211326896483No Hit
CATAAAGTAGAAAGCACTACT642250.7859441731830585No Hit
TCGTACGACTCTTAGCGG612670.7497460748681425No Hit
TGAGGTAGTAGATTGTATAGTT610810.7474699266982391No Hit
GCCTGTCTGAGCGTCGCG590790.7229707404823965No Hit
TATTGCACTTGTCCCGGCCTGTAA577680.7069275670913029No Hit
TATTGCACTTGTCCCGGCCTGT565920.6925364367267521No Hit
TATTGCACTTGTCCCGGCCTGTAT555960.6803480303975917No Hit
AACCGGGCGGAAACACCA484270.5926184270102915No Hit
TTCAAGTAATTCAGGATAGGTT474800.5810296511129875No Hit
TAGCTTATCAGACTGATGTTGAC449060.5495306974069043No Hit
TAGCTTATCAGACTGATGTTGA438590.5367181859343833No Hit
TAGCTTATCAGACTGATGTTGACT400270.48982463869207143No Hit
CGCCTGTCTGAGCGTCGCT385790.4721049475629306No Hit
TGTAAACATCCTTGACTGGAAGCT353790.4329454091559896No Hit
CAACGGAATCCCAAAAGCAGCTGT315740.38638227051898627No Hit
GCCTGTCTGAGCGTCGCA311530.38123034374732306No Hit
TATTGCACTTGTCCCGGCCTGTA297910.3645630652128688No Hit
TGAGGTAGTAGTTTGTACAGTT270160.3306044030005996No Hit
CCCTGTCTGAGCGTCGCT262070.32070438219709485No Hit
CCCATAAAGTAGAAAGCACTAC233090.28524052522730886No Hit
GCCGGTCTGAGCGTCGCT222090.27177943389992293No Hit
GCCTGTCTGAGCGTCGCTT218750.2676921570786984No Hit
GGAATACCGGGTGCTGTAGGCTTT216320.2647184796309213No Hit
CTACGCCTGTCTGAGCGTCGCT201410.24647258220443727No Hit
AGCAGCATTGTACAGGGCTATGA200940.24589742648408533No Hit
TTCTCACTACTGCACTTGACTAG189510.23191012885935605No Hit
CATTGCACTTGTCTCGGTCTGA186870.22867946694078342No Hit
TGATGTAGTAGGTTGTATAGTT183420.2244575792062851No Hit
TGTAAACATCCCCGACTGGAAGCT178520.21846127488772227No Hit
GGAATACCGGGTGCTGTAGGCTT167420.20487781000281458No Hit
ATCCCGGACGAGCCCCCA157120.19227333357808044No Hit
CTCCCACTGCTTCACTTGACTAG156930.19204082381878923No Hit
TGAGGTAGTAGTTTGTGCTGTT156050.19096393651259835No Hit
CAACACCAGTCGATGGGCTGT152640.1867909982011087No Hit
CCCATAAAGTAGAAAGCACT147540.18054994676750247No Hit
ACTGGACTTGGAGTCAGAAGGC138850.16991568461886755No Hit
TAGCAGCACGTAAATATTGGC137590.16837377779409426No Hit
TACGCCTGTCTGAGCGTCGCT126640.15497387324546913No Hit
CACCCGGCTGTGTGCACATGTGC126510.15481478762069092No Hit
NCCTGTCTGAGCGTCGCT119330.14602836619063353No Hit
TTCACAGTGGCTAAGTTCCGC118320.14479239325966445No Hit
TTCAAGTAATCCAGGATAGGC117690.1440214398472778No Hit
CTTCTCACTACTGCACTTGACTAG113390.1387593768738451No Hit
TGTAAACATCCTACACTCTCAGCT109940.13453748913934677No Hit
AGCTCGGTCTGAGGCCCCTCAGT108820.13316690529510383No Hit
TTCACAGTGGCTAAGTTCCG108060.13223686625793898No Hit
CAACGGAATCCCAAAAGCAGCT107470.13151486226856102No Hit
ACCGGGTGCTGTAGGCTT106860.1307683835676787No Hit
ACGCCTGTCTGAGCGTCGCT105950.12965478419423132No Hit
AACGGAATCCCAAAAGCAGCTG104130.12742758544733654No Hit
ACCTGTCTGAGCGTCGCT103300.12641188491990651No Hit
TGATGTAGTAGATTGTATAGTT102780.12577554242079372No Hit
TCCGGTCTGAGCGTCGCT100760.12330359655885556No Hit
TGAGGTAGTAGGTTGTATAGT97800.11968133925621352No Hit
TTCACAGTGGCTAAGTTCTGC94060.11510456820490228No Hit
ACCGGGTGCTGTAGGCTTT92550.11325672748632475No Hit
TATTGCACTTGTCCCGGCCTGTT91080.1114578361907559No Hit
AACATTCAACGCTGTCGGTGAGTT85130.10417660951821531No Hit
ACCCCCCACTGCTAAATTTGACTG84660.10360145379786337No Hit
ACCGGGTGCTGTAGGCTTA83730.10246337971291163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)