FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10039664
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7093617.065585063404513No Hit
CCCCCCACTGCTAAATTTGACTG5993855.970169918037098No Hit
AAGCTGCCAGTTGAAGAACTGT3939273.9237070085214008No Hit
GCCTGTCTGAGCGTCGCT3865713.850437624207344No Hit
TAGCAGCACGTAAATATTGGCG3782113.7671679052207327No Hit
AACATTCAACGCTGTCGGTGAGT3757643.742794579579556No Hit
TTCAAGTAATCCAGGATAGGCT3223753.2110138347259434No Hit
TAGCTTATCAGACTGATGTTGAC2794772.7837286188063666No Hit
AACATTCAACGCTGTCGGTGAG1925541.9179327116923435No Hit
CCCCCACTGCTAAATTTGACTG1598351.5920353509838576No Hit
TAGCTTATCAGACTGATGTTGACT1537671.5315950812696522No Hit
TAGCTTATCAGACTGATGTTGA1480641.4747903913915843No Hit
TATTGCACTTGTCCCGGCCTGTAA1438671.4329862035223488No Hit
TATTGCACTTGTCCCGGCCTGTAT1380641.3751854643741064No Hit
TATTGCACTTGTCCCGGCCTGT1311591.306408262268538No Hit
TGAGGTAGTAGGTTGTATAGTT1136751.1322590078711798No Hit
TGTAAACATCCTTGACTGGAAGCT1126041.121591320187608No Hit
TTCAAGTAATTCAGGATAGGTT981860.9779809364138083No Hit
AGCAGCATTGTACAGGGCTATGA903470.8999006341248074No Hit
TTAATGCTAATCGTGATAGGGGTT900180.8966236320259323No Hit
TCGTACGACTCTTAGCGG891350.8878285169702891No Hit
TGAGGTAGTAGATTGTATAGTT811700.8084931926008679No Hit
TGTAAACATCCCCGACTGGAAGCT750520.747554898251575No Hit
TATTGCACTTGTCCCGGCCTGTA708950.7061491300904094No Hit
ACTGGACTTGGAGTCAGAAGGC584640.5823302453149827No Hit
CATTGCACTTGTCTCGGTCTGA536450.5343306309852601No Hit
CATAAAGTAGAAAGCACTACT504190.5021980815294217No Hit
TGTAAACATCCTACACTCTCAGCT458800.45698740515618846No Hit
CAACGGAATCCCAAAAGCAGCTGT405820.4042167148223287No Hit
TGAGGTAGTAGTTTGTGCTGTT398120.39654713544198295No Hit
TCCTGTCTGAGCGTCGCT371470.37000242239182507No Hit
AACCGGGCGGAAACACCA347220.3458482275900867No Hit
AACATTCAACGCTGTCGGTGAGTT346170.34480237585640316No Hit
TAGCTTATCAGACTGATGTTGACA335170.3338458338844806No Hit
CACTAGATTGTGAGCTCCTGGA331060.32975207138406226No Hit
CCCATAAAGTAGAAAGCACTAC309170.30794855285993633No Hit
CAAAGAATTCTCCTTTTGGGCT278180.27708098597722No Hit
CCCATAAAGTAGAAAGCACT242930.241970249203559No Hit
CACTAGATTGTGAGCTCCTGGAA240340.23939048159380633No Hit
CAACGGAATCCCAAAAGCAGCT238960.23801593360096512No Hit
CAAAGAATTCTCCTTTTGGGCTT237860.23692027940377286No Hit
TTCAAGTAATCCAGGATAGGCTT234900.23397197356405555No Hit
TGAGGTAGTAGTTTGTACAGTT224430.2235433377053256No Hit
CTTCTCACTACTGCACTTGACTAG218480.21761684454778565No Hit
TATTGCACTTGTCCCGGCCTGTT216600.21574427191985707No Hit
TTCTCACTACTGCACTTGACTAG215060.2142103560437879No Hit
TGTAAACATCCCCGACTGGAAGC208810.20798504810519555No Hit
CACCCGGCTGTGTGCACATGTGC191740.19098248706331208No Hit
TAGCAGCACGTAAATATTGGC190720.1899665168077338No Hit
AGCTCGGTCTGAGGCCCCTCAGT185610.18487670503714068No Hit
CGCCTGTCTGAGCGTCGCT181810.18109171781047653No Hit
TTATAAAGCAATGAGACTGATT179560.17885060695258329No Hit
AGCAGCATTGTACAGGGCTATGT170340.16966703268157182No Hit
TGAGAACTGAATTCCATAGGCTGT168130.16746576379448555No Hit
TGAGAACTGAATTCCATGGGTT167260.16659920092943348No Hit
TATTGCACTTGTCCCGGCCTGTAG163410.1627644112392606No Hit
TGTAAACATCCTACACTCAGCT162470.1618281249252963No Hit
CAAAGTGCTGTTCGTGCAGGTAG157530.1569076415306329No Hit
CCCCCACTGCTAAATTTGACTGGCTT157270.15664866872038746No Hit
TCGTTTCCCGGCCAATGCACCA152560.15195727665786424No Hit
AACGGAATCCCAAAAGCAGCTG151630.1510309508366017No Hit
CTCCCACTGCTTCACTTGACTAG149750.14915837820867311No Hit
AGCAGCATTGTACAGGGCTATG145450.14487536634692155No Hit
TTCACAGTGGCTAAGTTCCG145110.14453670959506215No Hit
CCCCCCACTGCTAAATTTGACTGG142010.14144895685752032No Hit
TTCAAGTAATCCAGGATAGGC138420.13787313997759287No Hit
ACCACAGGGTAGAACCACGGAC137560.13701653760524257No Hit
TAGCAGCACGTAAATATTGGCGT135550.13501447857219126No Hit
CAACACCAGTCGATGGGCTGT134930.1343969280246829No Hit
TTAATGCTAATCGTGATAGGGGT130710.13019360010454534No Hit
ACCCCCCACTGCTAAATTTGACTG129810.12929715576138803No Hit
TGTCAGTTTGTCAAATACCCCA128400.1278927262904416No Hit
CAACGGAATCCCAAAAGCAGCTGA126120.1256217339544431No Hit
CCCCCCACTGCTAAATTTGACTGGCT125780.12528307720258366No Hit
ACCGGGTGCTGTAGGCTT125600.12510378833395222No Hit
TATTGCACTTGTCCCGGCCTGTAAT123400.12291247993956769No Hit
AACATTCAACGCTGTCGGTGA122270.1217869442642702No Hit
GACCCGCGGGCGCTCTCCAGTCCT120560.12008370001227132No Hit
GGAATACCGGGTGCTGTAGGCTTT119420.11894820384427207No Hit
TGTAAACATCCTTGACTGGAAGC118600.11813144344272877No Hit
TATTGCACTTGTCCCGGCCTGTTT117190.11672701397178233No Hit
TGTCAGTTTGTCAAATACCCCAA112220.11177664909901366No Hit
ACTGGACTTGGAGTCAGAAGG112050.11160732072308396No Hit
CAGTGCAATAGTATTGTCAAAGC110990.11055150849669869No Hit
CGCGACCTCAGATCAGACGTGG109390.10895782966441904No Hit
AACATTCAACGCTGTCGGTGAGA109080.10864905439066487No Hit
AACATTCATTGCTGTCGGTGGGT109020.10858929143445438No Hit
TGAGGTAGTAGGTTGTGTGGTT107940.10751355822266563No Hit
TATTGCACTTGTCCCGGCCTGA107090.10666691634301706No Hit
TGAGGTAGTAGTTTGTGCTGTTA106440.10601948431740345No Hit
CCCATAAAGTAGAAAGCACTA106120.10570074855094753No Hit
GCGTTCGCGCTTTCCCCTG105660.10524256588666711No Hit
CGCGACCTCAGATCAGACGTGGCG105660.10524256588666711No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT104610.1041967141529836No Hit
AACATTCATTGCTGTCGGTGGGTT104160.10374849198140496No Hit
TGAGGTAGTAGGTTGTATAGT104080.10366880803979098No Hit
CTACGCCTGTCTGAGCGTCGCT102450.10204524772940608No Hit
ACCGGGTGCTGTAGGCTTT101980.10157710457242394No Hit
ATCACATTGCCAGGGATTTCCA101970.1015671440797222No Hit
CAAAGTGCTGTTCGTGCAGGT101950.1015472230943187No Hit
AATGACACGATCACTCCCGTTG101370.10096951451761733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)