FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8614368
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT209230324.288525867480935No Hit
CAACGGAATCCCAAAAGCAGCTG6383487.410270840530611No Hit
CCCCCCACTGCTAAATTTGACTG3454184.009789226557305No Hit
TTCAAGTAATCCAGGATAGGCT2952233.42709993350644No Hit
TAGCAGCACGTAAATATTGGCG2429782.820613189499218No Hit
AAGCTGCCAGTTGAAGAACTGT2321592.695020691012968No Hit
AACATTCAACGCTGTCGGTGAGT1985582.3049630570693056No Hit
CCCCCACTGCTAAATTTGACTG1557811.8083857109424628No Hit
AACATTCAACGCTGTCGGTGAG1454811.6888180305276022No Hit
TGAGGTAGTAGGTTGTATAGTT1243201.443170294094703No Hit
CATAAAGTAGAAAGCACTACT865041.004182779282241No Hit
TAGCTTATCAGACTGATGTTGAC820920.9529660214190988No Hit
TCGTACGACTCTTAGCGG820280.9522230766087542No Hit
TAGCTTATCAGACTGATGTTGA788850.9157375213132292No Hit
TAGCTTATCAGACTGATGTTGACT771250.8953065390287482No Hit
TGAGGTAGTAGATTGTATAGTT629380.7306165698981051No Hit
TATTGCACTTGTCCCGGCCTGTAA617560.7168953079320503No Hit
TATTGCACTTGTCCCGGCCTGT577090.6699156571904056No Hit
CGCCTGTCTGAGCGTCGCT576820.6696022273485415No Hit
TATTGCACTTGTCCCGGCCTGTAT520770.6045365138800665No Hit
AACCGGGCGGAAACACCA516670.5997770236887954No Hit
TTCAAGTAATTCAGGATAGGTT483980.5618287957979041No Hit
TGTAAACATCCTTGACTGGAAGCT482280.5598553486454259No Hit
CCCATAAAGTAGAAAGCACTAC375880.43634077392560894No Hit
CAACGGAATCCCAAAAGCAGCTGT372610.4325447902852536No Hit
TTAATGCTAATCGTGATAGGGGTT352110.408747339328898No Hit
AGCAGCATTGTACAGGGCTATGA351310.407818658315967No Hit
CCCATAAAGTAGAAAGCACT332040.3854490544169926No Hit
CAACACCAGTCGATGGGCTGT314830.3654708041263155No Hit
TATTGCACTTGTCCCGGCCTGTA303930.3528175253251312No Hit
CATTGCACTTGTCTCGGTCTGA303250.35202814646413993No Hit
TGTAAACATCCCCGACTGGAAGCT253510.2942874044851578No Hit
TGAGGTAGTAGTTTGTACAGTT233980.2716159792569809No Hit
ACTGGACTTGGAGTCAGAAGGC224720.2608664965323051No Hit
ACCGGGTGCTGTAGGCTT220630.2561186148536956No Hit
TGAGAACTGAATTCCATAGGCTGT196720.2283626610797217No Hit
CAACGGAATCCCAAAAGCAGCT187330.21746226769044463No Hit
ATCCCGGACGAGCCCCCA178680.2074209042381287No Hit
TGATGTAGTAGGTTGTATAGTT178360.20704943183295627No Hit
TGAGGTAGTAGTTTGTGCTGTT177040.20551710816162022No Hit
ACCGGGTGCTGTAGGCTTT174680.2027774991734739No Hit
TAGCAGCACGTAAATATTGGC171970.1996315922421703No Hit
CACCCGGCTGTGTGCACATGTGC170790.19826178774809713No Hit
TGTAAACATCCTACACTCTCAGCT170160.197530451450414No Hit
TTCACAGTGGCTAAGTTCCGC166700.19351390606948762No Hit
TGAGAACTGAATTCCATGGGTT159860.18557368340892796No Hit
TCCTGTCTGAGCGTCGCT154790.17968816748947805No Hit
GCCGGTCTGAGCGTCGCT153850.17859696729928418No Hit
AACGGAATCCCAAAAGCAGCTG146090.16958876147385393No Hit
TTCTCACTACTGCACTTGACTAG144980.1683002165684122No Hit
TTCACAGTGGCTAAGTTCCG144130.16731349299217307No Hit
ACCGGGTGCTGTAGGCTTA141170.16387737324432855No Hit
CTACGCCTGTCTGAGCGTCGCT141070.16376128811771218No Hit
GCCTGTCTGAGCGTCGCTT140740.16337820719987817No Hit
CACCCGTAGAACCGACCTTGCG134680.15634344852692617No Hit
TTCAAGTAATCCAGGATAGGC133930.1554728100773034No Hit
AACATTCAACGCTGTCGGTGAGTT129350.1501561112782737No Hit
GCCTGTCTGAGCGTCGCG124120.14408485915623756No Hit
ACGCCTGTCTGAGCGTCGCT121680.14125238206679816No Hit
TACGCCTGTCTGAGCGTCGCT117700.13663219402746668No Hit
AGCTCGGTCTGAGGCCCCTCAGT117230.13608659393236974No Hit
TTCAAGTAATCCAGGATAGGCTT116310.13501861076749913No Hit
TCGTTTCCCGGCCAATGCACCA114800.13326572535559197No Hit
TTCACAGTGGCTAAGTTCTGC112600.13071185257003182No Hit
CAAAGAATTCTCCTTTTGGGCT112290.1303519886775211No Hit
TGAGGTAGTAGGTTGTATAGT109580.12720608174621748No Hit
TTATAAAGCAATGAGACTGATT109240.1268113923157218No Hit
GCCTGTCTGAGCGTCGCA107920.12527906864438573No Hit
TGAGGTAGTAGGTTGTGTGGTT104440.12123930623813609No Hit
TTCACAGTGGCTAAGTTCTG101190.11746653962310409No Hit
TGAGGTAGTAAGTTGTATTGTT97450.11312495588765188No Hit
CCCATAAAGTAGAAAGCACTA95930.11136046196308308No Hit
TGATGTAGTAGATTGTATAGTT89930.10439535436610092No Hit
TGTAAACATCCTACACTCAGCT87990.10214330290974334No Hit
CACTAGATTGTGAGCTCCTGGA87060.10106371123221113No Hit
GACCCGCGGGCGCTCTCCAGTCCT86540.10046006857380599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)