FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8157067
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT209955525.739092249702004No Hit
CAACGGAATCCCAAAAGCAGCTG5263756.452993459536375No Hit
CCCCCCACTGCTAAATTTGACTG3235973.967075420613807No Hit
TTCAAGTAATCCAGGATAGGCT2387162.9264930642349753No Hit
TAGCAGCACGTAAATATTGGCG2193212.6887237777990545No Hit
AAGCTGCCAGTTGAAGAACTGT2023772.481002056253798No Hit
AACATTCAACGCTGTCGGTGAGT1664162.04014506684817No Hit
AACATTCAACGCTGTCGGTGAG1454371.782957035905185No Hit
CCCCCACTGCTAAATTTGACTG1296101.5889289618437608No Hit
TCCTGTCTGAGCGTCGCT1178111.4442813820212583No Hit
TGAGGTAGTAGGTTGTATAGTT1017081.2468697388411791No Hit
TAGCTTATCAGACTGATGTTGA767440.9408283639205121No Hit
CATAAAGTAGAAAGCACTACT744050.9121538415707509No Hit
TAGCTTATCAGACTGATGTTGAC732700.8982395265357022No Hit
TAGCTTATCAGACTGATGTTGACT664980.8152194900446448No Hit
TATTGCACTTGTCCCGGCCTGTAA655710.8038551111569882No Hit
TGAGGTAGTAGATTGTATAGTT569610.6983024658250325No Hit
TATTGCACTTGTCCCGGCCTGT563750.691118511102091No Hit
TATTGCACTTGTCCCGGCCTGTAT546700.6702163902785155No Hit
TTCAAGTAATTCAGGATAGGTT477260.5850877527424992No Hit
TGTAAACATCCTTGACTGGAAGCT443060.5431609180113391No Hit
AACCGGGCGGAAACACCA371860.4558746421967602No Hit
TATTGCACTTGTCCCGGCCTGTA346940.4253244456616576No Hit
AGCAGCATTGTACAGGGCTATGA334200.4097060867588803No Hit
TCGTACGACTCTTAGCGG333590.40895826894642395No Hit
CCCATAAAGTAGAAAGCACTAC295390.36212771085489426No Hit
CAACGGAATCCCAAAAGCAGCTGT291730.3576408039801561No Hit
TTCTCACTACTGCACTTGACTAG281640.34527116180362377No Hit
CGCCTGTCTGAGCGTCGCT248640.30481544408057454No Hit
CCCATAAAGTAGAAAGCACT245250.3006595385326613No Hit
CATTGCACTTGTCTCGGTCTGA236180.28954034581302324No Hit
TGTAAACATCCCCGACTGGAAGCT230260.28228283523967623No Hit
TGAGGTAGTAGTTTGTACAGTT224000.2746085081806978No Hit
GCCGGTCTGAGCGTCGCT220970.2708939377352178No Hit
TTCACAGTGGCTAAGTTCCG219060.2685524098306413No Hit
CAACACCAGTCGATGGGCTGT215060.26364868647027173No Hit
ACTGGACTTGGAGTCAGAAGGC194380.23829643669716088No Hit
CCCCCACTGCTAAATTTGACTGGCTT190490.23352756572920147No Hit
TGATGTAGTAGGTTGTATAGTT171630.21040651008505876No Hit
ACCGGGTGCTGTAGGCTT161160.19757101418929132No Hit
TGAGGTAGTAGTTTGTGCTGTT157660.1932802562489679No Hit
CTTCTCACTACTGCACTTGACTAG151800.18609630152602646No Hit
CTCCCACTGCTTCACTTGACTAG149010.18267595448216864No Hit
GCCTGTCTGAGCGTCGCTT147390.18068994652121895No Hit
TAGCAGCACGTAAATATTGGC146440.17952531222313117No Hit
CCCTGTCTGAGCGTCGCT145270.17809097314022307No Hit
ATCCCGGACGAGCCCCCA143560.17599463140366506No Hit
CAACGGAATCCCAAAAGCAGCT143020.17533262875001515No Hit
GCCTGTCTGAGCGTCGCG142620.17484225641397821No Hit
CACCCGGCTGTGTGCACATGTGC134590.16499803176803624No Hit
TGTAAACATCCTACACTCTCAGCT132510.16244809562064405No Hit
AGCTCGGTCTGAGGCCCCTCAGT130520.16000849324886016No Hit
CAAAGAATTCTCCTTTTGGGCT128510.15754437226027443No Hit
TTCAAGTAATCCAGGATAGGC125510.15386657973999723No Hit
ACCGGGTGCTGTAGGCTTT119380.1463516236902308No Hit
TTCACAGTGGCTAAGTTCCGC119370.1463393643818299No Hit
AACATTCAACGCTGTCGGTGAGTT119250.1461922526810188No Hit
AACGGAATCCCAAAAGCAGCTG111610.13682614106271285No Hit
TTCACAGTGGCTAAGTTCTG111110.13621317564266666No Hit
GACCCGCGGGCGCTCTCCAGTCCT108670.1332219043928412No Hit
TGAGGTAGTAGGTTGTATAGT103340.1266876930151487No Hit
ACCGGGTGCTGTAGGCTTA100070.12267889916804656No Hit
TATTGCACTTGTCCCGGCCTGTT98550.1208154842911061No Hit
GCCTGTCTGAGCGTCGCA97810.11990829546943774No Hit
TGATGTAGTAGATTGTATAGTT97310.11929533004939152No Hit
CCCATAAAGTAGAAAGCACTA97310.11929533004939152No Hit
TAGCTTATCAGACTGATGTTGACA96880.1187681797881518No Hit
TTCACAGTGGCTAAGTTCTGC96700.11854751223693517No Hit
TCGTTTCCCGGCCAATGCACCA96690.11853525292853423No Hit
TTATAAAGCAATGAGACTGATT91270.11189070777523343No Hit
TCCGGTCTGAGCGTCGCT90650.11113063065437613No Hit
CCCCCCACTGCTAAATTTGACTGGCT88750.10880136205820058No Hit
CTACGCCTGTCTGAGCGTCGCT86390.1059081652755825No Hit
TGAGGTAGTAGGTTGTGTGGTT83210.10200970520408868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)