FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10008418
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG130530413.042061192887827No Hit
CAACGGAATCCCAAAAGCAGCTG8714978.707639908724836No Hit
CCCCCACTGCTAAATTTGACTG3851333.8480906772678756No Hit
TTCAAGTAATCCAGGATAGGCT3377813.3749689511369327No Hit
TAGCAGCACGTAAATATTGGCG3250783.248045795049727No Hit
AACATTCAACGCTGTCGGTGAGT2651872.64963953344075No Hit
AAGCTGCCAGTTGAAGAACTGT2541802.5396621124337533No Hit
AACATTCAACGCTGTCGGTGAG2402252.4002294868179965No Hit
GCCTGTCTGAGCGTCGCT2134942.1331443191121715No Hit
TATTGCACTTGTCCCGGCCTGTAT2111632.1098539249659636No Hit
TAGCTTATCAGACTGATGTTGACT1950151.9485097444970825No Hit
TAGCTTATCAGACTGATGTTGAC1637141.6357630146942304No Hit
TATTGCACTTGTCCCGGCCTGTAA1604901.603550131499304No Hit
TGAGGTAGTAGGTTGTATAGTT1540871.5395739866180649No Hit
TAGCTTATCAGACTGATGTTGA1520441.5191611701269871No Hit
TATTGCACTTGTCCCGGCCTGT1119921.1189780442823232No Hit
TGAGGTAGTAGATTGTATAGTT1063911.0630151538434944No Hit
TGTAAACATCCTTGACTGGAAGCT921050.9202753122421546No Hit
TATTGCACTTGTCCCGGCCTGTA893220.8924687198316458No Hit
AGCAGCATTGTACAGGGCTATGA729510.7288964149978548No Hit
TTCAAGTAATTCAGGATAGGTT691180.6905986540530181No Hit
CATAAAGTAGAAAGCACTACT612840.6123245451978524No Hit
TGTAAACATCCCCGACTGGAAGCT603380.6028725019278771No Hit
ACTGGACTTGGAGTCAGAAGGC504060.5036360391822164No Hit
CAACGGAATCCCAAAAGCAGCTGT453650.45326843862836264No Hit
CACCCGGCTGTGTGCACATGTGC435010.4346441165826607No Hit
CATTGCACTTGTCTCGGTCTGA416940.41658931511453656No Hit
CTCCCACTGCTTCACTTGACTAG403320.4029807707871514No Hit
TGAGGTAGTAGTTTGTACAGTT376790.3764730849570831No Hit
CCCATAAAGTAGAAAGCACT357920.357618956362534No Hit
CCCCCACTGCTAAATTTGACTGGCTT350370.35007530660689834No Hit
TTCTCACTACTGCACTTGACTAG337300.33701629967893026No Hit
CTTCTCACTACTGCACTTGACTAG307520.30726134739776056No Hit
TGTAAACATCCTACACTCTCAGCT296980.2967302125071115No Hit
CCCCCCACTGCTAAATTTGACTGGCT294570.29432223953875625No Hit
CCCATAAAGTAGAAAGCACTAC291690.2914446618836264No Hit
CCCCCCACTGCTAAATTTGACTGG279340.2791050493694408No Hit
CAAAGAATTCTCCTTTTGGGCT276640.2764073203177565No Hit
TATTGCACTTGTCCCGGCCTGTT272030.2718011977517326No Hit
CACTAGATTGTGAGCTCCTGGAA265220.26499692558804No Hit
CACTAGATTGTGAGCTCCTGGA262990.26276880122313034No Hit
CAACGGAATCCCAAAAGCAGCT261570.261349995573726No Hit
CAACACCAGTCGATGGGCTGT259170.2589520141944511No Hit
AACGGAATCCCAAAAGCAGCTG252420.2522076915652404No Hit
TATTGCACTTGTCCCGGCCTGTAG242620.24241593426653443No Hit
TAGCTTATCAGACTGATGTTGACA242110.2419063632234385No Hit
TAGCAGCACGTAAATATTGGC239920.23971820521485016No Hit
AGCTCGGTCTGAGGCCCCTCAGT235020.23482232656549715No Hit
TGAGGTAGTAGTTTGTGCTGTT232740.23254424425518597No Hit
ACCCCCCACTGCTAAATTTGACTG229650.22945684322936952No Hit
TATTGCACTTGTCCCGGCCTGTTT226790.22659924875240023No Hit
CCCCCCACTGCTAAATTTGACTGT224180.22399144400243876No Hit
CCCCCCACTGCTAAATTTGACTGTA189580.18942054578455855No Hit
TGTAAACATCCCCGACTGGAAGC186740.1865829344857499No Hit
TTCAAGTAATCCAGGATAGGCTT185890.18573364941392337No Hit
AGCAGCATTGTACAGGGCTATGT182330.18217664370133224No Hit
TCGTACGACTCTTAGCGG182280.18212668575593066No Hit
AACATTCAACGCTGTCGGTGAGTT179610.17945893147148728No Hit
ACTGGACTTGGAGTCAGAAGGCT175850.17570209397728992No Hit
CAAAGAATTCTCCTTTTGGGCTT168230.16808850309809203No Hit
ACTGGACTTGGAGTCAGAAGGCA167870.1677288058912008No Hit
CCCCCCACTGCTAAATTTGACTGA166410.1662700338854752No Hit
TGAGAACTGAATTCCATAGGCTGT161250.1611143739200341No Hit
CTCCCACTGCTTCACTTGACTAGCCT155980.15584880647470958No Hit
ATCCCGGACGAGCCCCCA153680.1535507409862378No Hit
TTCAAGTAATCCAGGATAGGC151150.15102286894891878No Hit
TATTGCACTTGTCCCGGCCTGTAAT145500.1453776211185424No Hit
GGAATACCGGGTGCTGTAGGCTTT136210.13609543486293238No Hit
TGAGGTAGTAGGTTGTATAGT135130.13501634324225867No Hit
GACCCGCGGGCGCTCTCCAGTCCT132040.13192894221644222No Hit
TATTGCACTTGTCCCGGCCTGA128990.128881507546947No Hit
TGTCAGTTTGTCAAATACCCCA127340.12723289534869547No Hit
TGAGGTAGTAGGTTGTGTGGTT122930.1228266045642778No Hit
AACCGGGCGGAAACACCA121280.12117799236602628No Hit
TTATAAAGCAATGAGACTGATT120910.12080830357005473No Hit
ACTGGACTTGGAGTCAGAAGG116260.11616221464770957No Hit
TTCACAGTGGCTAAGTTCCG114890.11479336694370679No Hit
TCGTTTCCCGGGCGGCGCACCA109720.10962771538918538No Hit
TGAGGTAGTAAGTTGTATTGTT109520.10942788360757914No Hit
CGCCTGTCTGAGCGTCGCT109130.10903821163344696No Hit
GGAATACCGGGTGCTGTAGGCTT109110.10901822845528633No Hit
TGTCAGTTTGTCAAATACCCCAA107240.10714980129726795No Hit
TATTGCACTTGTCCCGGCCTGTAAA104830.10474182832891273No Hit
CCCATAAAGTAGAAAGCACTA103740.10365274511915869No Hit
TTCACAGTGGCTAAGTTCCGC102900.10281345163641246No Hit
AGCAGCATTGTACAGGGCTATG101920.10183427590654189No Hit
TCAGTGCACTACAGAACTTTGT101620.1015345282341325No Hit
ATCACATTGCCAGGGATTTCC101580.10149456187781127No Hit
CCCCCCACTGCTAAATTTGAC100420.10033553754449503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)