FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8135244
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6482527.968439545267481No Hit
CCCCCCACTGCTAAATTTGACTG6070137.461521744154201No Hit
TAGCAGCACGTAAATATTGGCG3717704.569869078296852No Hit
AACATTCAACGCTGTCGGTGAGT3167473.8935156708268366No Hit
TTCAAGTAATCCAGGATAGGCT3128883.8460800929879913No Hit
AAGCTGCCAGTTGAAGAACTGT3036403.7324018800173664No Hit
CCCCCACTGCTAAATTTGACTG2041012.5088491506831265No Hit
AACATTCAACGCTGTCGGTGAG2039782.5073372107831062No Hit
TAGCTTATCAGACTGATGTTGA1557671.91471823094673No Hit
TAGCTTATCAGACTGATGTTGAC1142641.4045552905358463No Hit
TAGCTTATCAGACTGATGTTGACT1102441.3551406694132346No Hit
TGAGGTAGTAGGTTGTATAGTT1101491.3539729109538694No Hit
TATTGCACTTGTCCCGGCCTGT911741.1207285239385567No Hit
TATTGCACTTGTCCCGGCCTGTAT894111.0990573853716004No Hit
TATTGCACTTGTCCCGGCCTGTAA884541.0872937554177846No Hit
TGAGGTAGTAGATTGTATAGTT727450.8941956750160167No Hit
TTCAAGTAATTCAGGATAGGTT726170.892622274144451No Hit
CATAAAGTAGAAAGCACTACT723460.8892910894866829No Hit
TGTAAACATCCTTGACTGGAAGCT609890.7496886387181503No Hit
AGCAGCATTGTACAGGGCTATGA488010.5998713744787495No Hit
TATTGCACTTGTCCCGGCCTGTA467220.5743159025101152No Hit
TTCTCACTACTGCACTTGACTAG442860.5443721171731296No Hit
CATTGCACTTGTCTCGGTCTGA384530.47267174776810633No Hit
CCCATAAAGTAGAAAGCACT366100.4500172336564213No Hit
GGAATACCGGGTGCTGTAGGCTTT359940.4424452419620112No Hit
TGTAAACATCCCCGACTGGAAGCT353650.4347134517415827No Hit
CTTCTCACTACTGCACTTGACTAG336890.4141117340795187No Hit
ACTGGACTTGGAGTCAGAAGGC336050.41307918975755364No Hit
CAACGGAATCCCAAAAGCAGCTGT329210.40467132885012425No Hit
CTCCCACTGCTTCACTTGACTAG315150.3873885036515192No Hit
GGAATACCGGGTGCTGTAGGCTT310170.38126699088558375No Hit
CACCCGGCTGTGTGCACATGTGC298350.36673761721221887No Hit
TGAGGTAGTAGTTTGTACAGTT292790.3599031571763551No Hit
TGATGTAGTAGGTTGTATAGTT292100.3590549957690267No Hit
CGCCTGTCTGAGCGTCGCT267830.32922184018082307No Hit
CCCATAAAGTAGAAAGCACTAC264360.32495644875556284No Hit
CAACACCAGTCGATGGGCTGT256070.3147662196733128No Hit
TTAATGCTAATCGTGATAGGGGTT246410.3028919599707151No Hit
CCCCCCACTGCTAAATTTGACTGG234180.2878586063306768No Hit
TGAGGTAGTAGTTTGTGCTGTT225610.2773241958077717No Hit
CAACGGAATCCCAAAAGCAGCT220650.27122726743045444No Hit
TAGCAGCACGTAAATATTGGC208420.2561939137904161No Hit
TGATGTAGTAGATTGTATAGTT193970.23843169301375594No Hit
TGAGAACTGAATTCCATGGGTT183360.225389674851793No Hit
TGTAAACATCCTACACTCTCAGCT181480.22307874232168082No Hit
AACATTCAACGCTGTCGGTGAGTT180790.22223058091435244No Hit
ATCCCGGACGAGCCCCCA174500.21449879069392386No Hit
TTCACAGTGGCTAAGTTCCG162780.20009233896365No Hit
AACCGGGCGGAAACACCA160750.1975970235189012No Hit
AACGGAATCCCAAAAGCAGCTG159260.1957654865668442No Hit
CAAAGAATTCTCCTTTTGGGCT158310.19459772810747902No Hit
TTCAAGTAATCCAGGATAGGC156670.1925818082407854No Hit
TTCAAGTAATCCAGGATAGGCTT155990.1917459390277661No Hit
CCCCCACTGCTAAATTTGACTGGCTT148940.1830799420398454No Hit
TATTGCACTTGTCCCGGCCTGTT146980.18067067195526035No Hit
CACTAGATTGTGAGCTCCTGGA145290.1785932911170212No Hit
CCCCCCACTGCTAAATTTGACTGGCT144140.17717968877147386No Hit
TGTAAACATCCCCGACTGGAAGC142570.175249814264944No Hit
AACATTCAACGCTGTCGGTGA139150.17104588381122926No Hit
TGAATACCGGGTGCTGTAGGCTTT137610.16915288588762672No Hit
TAGCTTATCAGACTGATGTTGACA134230.16499812421114843No Hit
GCCTGTCTGAGCGTCGCT133390.1639655798891834No Hit
TCGTACGACTCTTAGCGG132470.16283469801274553No Hit
TGTCAGTTTGTCAAATACCCCAA130920.16092940789483387No Hit
CAAAGAATTCTCCTTTTGGGCTT130640.16058522645417886No Hit
TAGTGGGTTATCAGAACT125870.15472184976873465No Hit
TGAGGTAGTAGGTTGTATAGT123600.15193152166056728No Hit
CTACGCCTGTCTGAGCGTCGCT122640.15075147100689298No Hit
TGTCAGTTTGTCAAATACCCCA118990.14626482008406877No Hit
TGAATACCGGGTGCTGTAGGCTT118150.14523227576210376No Hit
TTATAAAGCAATGAGACTGATT117770.14476517237835768No Hit
TTCACAGTGGCTAAGTTCTG116940.14374492025070176No Hit
AATGACACGATCACTCCCGTTG115000.14136023455473493No Hit
CAAAGTGCTGTTCGTGCAGGT114730.14102834530838904No Hit
AGCTCGGTCTGAGGCCCCTCAGT112490.13827489378314897No Hit
ACCGGGTGCTGTAGGCTT112350.13810280306282147No Hit
TTCACAGTGGCTAAGTTCTGC108620.13351781458552442No Hit
TATTGCACTTGTCCCGGCCTGTAG107630.13230088734892279No Hit
AGCAGCATTGTACAGGGCTATG106560.13098562255784832No Hit
TGAGGTAGTAGGTTGTGTGGTT106050.13035872064808382No Hit
AGCAGCATTGTACAGGGCTATGT105310.12944909826920986No Hit
ACTGGACTTGGAGTCAGAAGG103180.12683086088137002No Hit
CCCATAAAGTAGAAAGCACTA102830.12640063408055124No Hit
ACCCCCCACTGCTAAATTTGACTG99580.12240567093009135No Hit
ATCCCACCGCTGCCACCA97460.11979972573656057No Hit
ACTGGACTTGGAGTCAGAAGGCT95130.11693564446253857No Hit
CACTAGATTGTGAGCTCCTGGAA95050.1168373069080657No Hit
ACCACAGGGTAGAACCACGGAC94750.11646854107879247No Hit
TGAGAACTGAATTCCATAGGCTGT92390.11356758322184314No Hit
ACTTGACTTGGAGTCAGAAGGC91970.11305131106086061No Hit
TATTGCACTTGTCCCGGCCTGA91810.1128546359519149No Hit
CTCCCACTGCTTCACTTGACTAGCCT90910.11174833846409524No Hit
TCGTTTCCCGGCCAATGCACCA89260.10972012640309253No Hit
CCCCCACTGCTAAATTTGACTGG88280.10851549136079999No Hit
TAGCAGCACGTAAATATTGGCGT88000.108171309920145No Hit
TGAGGTAGTAGATTGTATAGT87660.10775337531363532No Hit
CCCCCCACTGCTAAATTTGACTGTA87650.10774108311932622No Hit
CCCCCCACTGCTAAATTTGACTGT87330.10734773290143479No Hit
ACTGGACTTGGAGTCAGAAGGCA85790.10545473497783225No Hit
TAGCTTATCAGACTGATGTTG85770.10543015058921404No Hit
AACATTCAACGCTGTCGGTGAGA83530.10267669906397399No Hit
ACCGGGTGCTGTAGGCTTT83100.10214813470868238No Hit
TTCACAGTGGCTAAGTTCCGC82250.10110329819240825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)