FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8975673
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6694277.458237393452278No Hit
CAACGGAATCCCAAAAGCAGCTG6036246.7251113092021075No Hit
AACATTCAACGCTGTCGGTGAGT3586733.99605689734909No Hit
AAGCTGCCAGTTGAAGAACTGT3549953.9550794686927655No Hit
TTCAAGTAATCCAGGATAGGCT3544463.948962935704097No Hit
TAGCAGCACGTAAATATTGGCG3002973.3456766974465313No Hit
CCCCCACTGCTAAATTTGACTG2207012.4588796851222186No Hit
AACATTCAACGCTGTCGGTGAG1919502.138558300865016No Hit
TAGCTTATCAGACTGATGTTGAC1434411.5981085763708194No Hit
TAGCTTATCAGACTGATGTTGA1246031.3882301639108288No Hit
TGAGGTAGTAGGTTGTATAGTT1228011.3681536749389154No Hit
TAGCTTATCAGACTGATGTTGACT1226321.3662708077711834No Hit
TATTGCACTTGTCCCGGCCTGTAA778010.8667985119333113No Hit
CATAAAGTAGAAAGCACTACT773260.861506429657141No Hit
GGAATACCGGGTGCTGTAGGCTTT723770.8063685029523692No Hit
TGTAAACATCCTTGACTGGAAGCT723550.8061233959837886No Hit
TATTGCACTTGTCCCGGCCTGTAT706280.786882498950218No Hit
TATTGCACTTGTCCCGGCCTGT675530.7526232294781684No Hit
TGAGGTAGTAGATTGTATAGTT648330.7223190951809407No Hit
CGCCTGTCTGAGCGTCGCT639320.7122808506949841No Hit
AGCAGCATTGTACAGGGCTATGA608210.67762049709253No Hit
GGAATACCGGGTGCTGTAGGCTT549970.6127339977737603No Hit
GCCTGTCTGAGCGTCGCT419670.46756382501902644No Hit
ACTGGACTTGGAGTCAGAAGGC418990.46680622166159574No Hit
CCCATAAAGTAGAAAGCACTAC417210.46482308346126244No Hit
TTCAAGTAATTCAGGATAGGTT414570.4618817998382962No Hit
TATTGCACTTGTCCCGGCCTGTA412330.4593861652491128No Hit
TGTAAACATCCCCGACTGGAAGCT409100.45578754930131704No Hit
TTAATGCTAATCGTGATAGGGGTT406710.453124796324465No Hit
CAACGGAATCCCAAAAGCAGCTGT403420.44945933302160185No Hit
TGTAAACATCCTACACTCTCAGCT352210.3924051154715641No Hit
AACATTCAACGCTGTCGGTGAGTT310740.3462024518941365No Hit
CCCCCACTGCTAAATTTGACTGGCTT302860.33742316592861615No Hit
TGAGGTAGTAGTTTGTACAGTT298900.33301124049416686No Hit
CATTGCACTTGTCTCGGTCTGA297990.33199738894231107No Hit
TAGCAGCACATCATGGTTTACA284550.3170235814072104No Hit
CCCATAAAGTAGAAAGCACT281570.3137034961055288No Hit
CTACGCCTGTCTGAGCGTCGCT270140.3009690749651865No Hit
CAACGGAATCCCAAAAGCAGCT268530.2991753376042109No Hit
TTCTCACTACTGCACTTGACTAG246850.27502115997318527No Hit
CCCCCCACTGCTAAATTTGACTGG242240.269885054858839No Hit
TCCACCACGTTCCCGTGG238710.26595220213570614No Hit
CCCCCCACTGCTAAATTTGACTGGCT233480.2601253410189966No Hit
TACGCCTGTCTGAGCGTCGCT229790.2560142286823506No Hit
CTCCCACTGCTTCACTTGACTAG225500.25123464279503055No Hit
CAACACCAGTCGATGGGCTGT224380.2499868255004388No Hit
TGAGGTAGTAGTTTGTGCTGTT219750.24482843793440334No Hit
CACCCGGCTGTGTGCACATGTGC218130.2430235593475832No Hit
TTCACAGTGGCTAAGTTCCG215860.24049450108086604No Hit
CTTCTCACTACTGCACTTGACTAG215860.24049450108086604No Hit
TCGTTTCCCGGCCAATGCACCA207650.23134755466247492No Hit
CAAAGAATTCTCCTTTTGGGCT181420.20212411927217044No Hit
ATCACATTGCCAGGGATTTCC180460.20105456159109186No Hit
GCGTTCGCGCTTTCCCCTG180270.20084287830004502No Hit
ACGCCTGTCTGAGCGTCGCT178410.19877061029295517No Hit
TAGCTTATCAGACTGATGTTGACA177000.19719969744887095No Hit
TGAATACCGGGTGCTGTAGGCTTT174400.19430297872928304No Hit
TAGCAGCACGTAAATATTGGC174010.19386847092134485No Hit
TTCAAGTAATCCAGGATAGGCTT161100.1794851483560063No Hit
TTCAAGTAATCCAGGATAGGC157120.17505094046986783No Hit
AACGGAATCCCAAAAGCAGCTG153800.17135205348947094No Hit
TTCACAGTGGCTAAGTTCCGC153610.17114037019842412No Hit
TGTAAACATCCTACACTCAGCT150630.16782028489674256No Hit
TGAGGTAGTAGGTTGTGTGGTT150310.16746376566971635No Hit
AGCTCGGTCTGAGGCCCCTCAGT142030.15823883067041325No Hit
GTCAGGATGGCCGAGCGGTCTAAGG139030.15489646291704254No Hit
TAGCAGCACGTAAATATTGGCGT135100.15051796116012695No Hit
TCAGGCTCAGTCCCCTCCCGAT134570.14992747619036476No Hit
TGATGTAGTAGGTTGTATAGTT130880.14581636385371882No Hit
TGAGGTAGTAGGTTGTATAGT129500.1442788746871683No Hit
TGAATACCGGGTGCTGTAGGCTT129160.14390007300845298No Hit
AGCAGCATTGTACAGGGCTATGT128270.1429085039082863No Hit
TATTGCACTTGTCCCGGCCTGTT124180.1383517425378576No Hit
CCCCCCACTGCTAAATTTGACTGTA123460.13754957427704864No Hit
AACCGGGCGGAAACACCA123260.13732674976015727No Hit
ACTGGACTTGGAGTCAGAAGGCA123060.1371039252432659No Hit
TGTCAGTTTGTCAAATACCCCA120220.13393981710340827No Hit
ATCACATTGCCAGGGATTTCCA117590.13100967470628663No Hit
TTCACAGTGGCTAAGTTCTGC117340.13073114406017242No Hit
TTATAAAGCAATGAGACTGATT117300.13068657915679416No Hit
GGGCTACGCCTGTCTGAGCGTCGCT116100.12934963205544586No Hit
CAAAGAATTCTCCTTTTGGGCTT114320.1273664938551126No Hit
ACCCCCCACTGCTAAATTTGACTG113290.126218947593122No Hit
ACCGGGTGCTGTAGGCTT113180.12609639410883172No Hit
ACTGGACTTGGAGTCAGAAGG112670.12552819159075873No Hit
ACCACAGGGTAGAACCACGGAC112250.1250602601052868No Hit
TAGCAGCACATAATGGTTTGT109610.1221189764823206No Hit
AGCAGCATTGTACAGGGCTATG109120.12157305641593671No Hit
ACTGGACTTGGAGTCAGAAGGCT108190.12053692241239179No Hit
ATCCCGGACGAGCCCCCA107350.119601059441448No Hit
TGTAAACATCCCCGACTGGAAGC106670.11884345608401733No Hit
GGAATACCGGGTGCTGTAGGCTTA105860.11794101679060724No Hit
AACATTCAACGCTGTCGGTGA105200.11720569588486568No Hit
CAAAGTGCTGTTCGTGCAGGT105090.11708314240057541No Hit
TCCTGTCTGAGCGTCGCT101800.11341767909771223No Hit
AACATTCAACGCTGTCGGTGAGA101370.11293860638639576No Hit
TGTCAGTTTGTCAAATACCCCAA100490.1119581785120737No Hit
TCAGGCTCAGTCCCCTCCCGA99840.1112339988321767No Hit
CCCCCCACTGCTAAATTTGACTGT97040.10811445559569738No Hit
TCGTACGACTCTTAGCGG95200.1060644700402967No Hit
AGAGGTAGTAGGTTGCATAGTT94110.10485007642323868No Hit
TATTGCACTTGTCCCGGCCTGTAG92870.10346856441851213No Hit
TTCACAGTGGCTAAGTTCTG92830.10342399951513384No Hit
TTCAAGTAATCCAGGATAGGCA91760.10223188834976497No Hit
CTCCCACTGCTTCACTTGACTAGCCT91330.1017528156384485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)