FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961328
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6216938.930666677392589No Hit
CCCCCCACTGCTAAATTTGACTG5782528.306633446951501No Hit
TAGCAGCACGTAAATATTGGCG2396733.4429206611152354No Hit
AACATTCAACGCTGTCGGTGAGT2330073.3471630700349126No Hit
AAGCTGCCAGTTGAAGAACTGT2239783.2174608063289076No Hit
TTCAAGTAATCCAGGATAGGCT2228063.2006249382301766No Hit
CCCCCACTGCTAAATTTGACTG1578772.2679149725454684No Hit
TAGCTTATCAGACTGATGTTGAC1510372.169657858385641No Hit
AACATTCAACGCTGTCGGTGAG1321991.8990485723413695No Hit
GCCTGTCTGAGCGTCGCT1233761.7723055141202944No Hit
TAGCTTATCAGACTGATGTTGACT1227601.7634566278158421No Hit
TAGCTTATCAGACTGATGTTGA955641.3727840435043428No Hit
TGTAAACATCCTTGACTGGAAGCT766371.1008962657699795No Hit
TATTGCACTTGTCCCGGCCTGTAA731491.0507908835785356No Hit
TGAGGTAGTAGGTTGTATAGTT658570.946040755442065No Hit
TATTGCACTTGTCCCGGCCTGTAT638480.9171813194264083No Hit
AGCAGCATTGTACAGGGCTATGA625900.8991100548630951No Hit
CCCCCACTGCTAAATTTGACTGGCTT481100.6911037664077888No Hit
TGTAAACATCCCCGACTGGAAGCT440330.6325373549414709No Hit
TCCTGTCTGAGCGTCGCT426260.6123256941778925No Hit
CAACGGAATCCCAAAAGCAGCTGT388900.5586577733443964No Hit
TGAGGTAGTAGATTGTATAGTT375580.5395234932185353No Hit
TATTGCACTTGTCCCGGCCTGT369870.5313210352967135No Hit
GGAATACCGGGTGCTGTAGGCTTT341420.4904523964393001No Hit
CATAAAGTAGAAAGCACTACT339780.4880965241114914No Hit
CCCCCCACTGCTAAATTTGACTGGCT317710.45639280321226067No Hit
ACTGGACTTGGAGTCAGAAGGC309100.44402447349126495No Hit
TATTGCACTTGTCCCGGCCTGTA307100.4411514584573518No Hit
CCCATAAAGTAGAAAGCACTAC304220.437014316808517No Hit
TTCAAGTAATTCAGGATAGGTT303800.43641098365139525No Hit
TGTAAACATCCTACACTCTCAGCT303690.43625296782453005No Hit
CTTCTCACTACTGCACTTGACTAG270880.3891211561931861No Hit
TAGCTTATCAGACTGATGTTGACA258990.3720410818165729No Hit
TTCTCACTACTGCACTTGACTAG255180.3665679881769685No Hit
CAAAGAATTCTCCTTTTGGGCT236120.339188154903777No Hit
GGAATACCGGGTGCTGTAGGCTT228210.32782538044465076No Hit
TGTCAGTTTGTCAAATACCCCAA223430.32095887451359856No Hit
AACATTCAACGCTGTCGGTGAGTT222540.31968038282350725No Hit
CCCCCCACTGCTAAATTTGACTGG220500.3167499074889159No Hit
CTCCCACTGCTTCACTTGACTAG208470.29946872205992875No Hit
TGATGTAGTAGGTTGTATAGTT187360.26914404837697636No Hit
CAACGGAATCCCAAAAGCAGCT187010.2686412707460416No Hit
CATTGCACTTGTCTCGGTCTGA177890.255540322191398No Hit
TGTCAGTTTGTCAAATACCCCA168140.24153437390107174No Hit
TGAGGTAGTAGTTTGTACAGTT166610.23933651740012826No Hit
CTCCCACTGCTTCACTTGACTAGCCT160110.2299992185399108No Hit
TAGCAGCACGTAAATATTGGC157540.22630739422133248No Hit
CACCCGGCTGTGTGCACATGTGC155520.2234056490370803No Hit
CGCCTGTCTGAGCGTCGCT153470.22046080862731937No Hit
ACCCCCCACTGCTAAATTTGACTG146050.2098019228515019No Hit
TGAATACCGGGTGCTGTAGGCTTT143210.20572224150334534No Hit
CCCCCCACTGCTAAATTTGACTGTA141840.20375422620511488No Hit
CCCATAAAGTAGAAAGCACT138830.19943033857907572No Hit
CAAAGAATTCTCCTTTTGGGCTT137630.19770652955872786No Hit
TGTAAACATCCCCGACTGGAAGC136920.1966866092216887No Hit
ATCACATTGCCAGGGATTTCCA133140.191256610807593No Hit
TGAGGTAGTAGTTTGTGCTGTT132240.18996375404233215No Hit
TTCAAGTAATCCAGGATAGGCTT125630.18046843935524945No Hit
TAGCAGCACGTAAATATTGGCGT120750.17345828267250157No Hit
AGCAGCATTGTACAGGGCTATGT118950.17087256914197982No Hit
CTACGCCTGTCTGAGCGTCGCT117050.1681432048597624No Hit
TGTAAACATCCTACACTCAGCT117020.1681001096342537No Hit
ATCACATTGCCAGGGATTTCC114320.16422153933847106No Hit
AACGGAATCCCAAAAGCAGCTG111790.160587175320571No Hit
AACCGGGCGGAAACACCA111730.16050098486955364No Hit
AGCTCGGTCTGAGGCCCCTCAGT110280.15841804896996667No Hit
GTCAGGATGGCCGAGCGGTCTAAGG109290.15699590652817969No Hit
CCCCCACTGCTAAATTTGACTGGCTA108860.1563782082958884No Hit
TGATGTAGTAGATTGTATAGTT107260.15407979626875792No Hit
TTCACAGTGGCTAAGTTCCG107210.1540079708929101No Hit
ACTGGACTTGGAGTCAGAAGGCA105250.1511924161596753No Hit
TCGTTTCCCGGCCAATGCACCA99830.14340654541777087No Hit
TGAATACCGGGTGCTGTAGGCTT97680.1403180542563143No Hit
TTCAAGTAATCCAGGATAGGC97580.14017440350461866No Hit
GGGCTACGCCTGTCTGAGCGTCGCT96560.13870916583732298No Hit
CCCCCACTGCTAAATTTGACTGGCT95770.13757432489892732No Hit
TATTGCACTTGTCCCGGCCTGTT86870.12478940799801416No Hit
AACACCCTGATTGCTCCTATCTGATT86810.12470321754699679No Hit
ACTTGACTTGGAGTCAGAAGGC86750.12461702709597938No Hit
TTAATGCTAATCGTGATAGGGGTT85430.12272083717359676No Hit
CAACACCAGTCGATGGGCTGT83990.12065226634917935No Hit
TATTGCACTTGTCCCGGCCTGTAG83470.11990528244036196No Hit
CACTAGATTGTGAGCTCCTGGAA82050.11786544176628368No Hit
AGCAGCATTGTACAGGGCTATG81780.11747758473670541No Hit
ACTGGACTTGGAGTCAGAAGGCT81060.11644329932449671No Hit
AACATTCAACGCTGTCGGTGAGA78840.1132542526368532No Hit
CCCCCCACTGCTAAATTTGACTGT78670.11301004635897059No Hit
TAGCAGCACATCATGGTTTACA77750.11168845944337057No Hit
CAAAGTGCTGTTCGTGCAGGTAG74280.10670377835953139No Hit
CACTAGATTGTGAGCTCCTGGA73600.10572695324800095No Hit
TTCACAGTGGCTAAGTTCTGC72400.10400314422765312No Hit
ATGCACCTGGGCAAGGATTCTGA69860.10035441513458353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)