FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4593603
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG77066316.776874274942784No Hit
CAACGGAATCCCAAAAGCAGCTG4321029.406603052113995No Hit
CCCCCACTGCTAAATTTGACTG1757083.82505845629237No Hit
TATTGCACTTGTCCCGGCCTGTAT1232302.682643667726619No Hit
AACATTCAACGCTGTCGGTGAGT1057312.3017008653120437No Hit
TAGCAGCACGTAAATATTGGCG1050802.2875289832403887No Hit
TATTGCACTTGTCCCGGCCTGTAA1025162.2317122311179265No Hit
TTCAAGTAATCCAGGATAGGCT1023472.2280332018243634No Hit
AACATTCAACGCTGTCGGTGAG860491.8732354537386013No Hit
TAGCTTATCAGACTGATGTTGACT830611.8081884742760748No Hit
AAGCTGCCAGTTGAAGAACTGT745901.6237798521117304No Hit
GCCTGTCTGAGCGTCGCT688821.499520093486529No Hit
TAGCTTATCAGACTGATGTTGAC644601.4032557885389747No Hit
TATTGCACTTGTCCCGGCCTGTA420720.9158823694603125No Hit
TAGCTTATCAGACTGATGTTGA403230.8778076816825485No Hit
TATTGCACTTGTCCCGGCCTGT397230.8647460392201939No Hit
GGAATACCGGGTGCTGTAGGCTTT347110.7556377858513241No Hit
TGAGGTAGTAGGTTGTATAGTT318800.6940086028331137No Hit
TGTAAACATCCTTGACTGGAAGCT311010.6770502370361565No Hit
AGCAGCATTGTACAGGGCTATGA282280.6145067390455814No Hit
TGTAAACATCCCCGACTGGAAGCT261170.5685515269821967No Hit
TCCTGTCTGAGCGTCGCT238300.5187648997965213No Hit
TGAGGTAGTAGATTGTATAGTT237680.517415196742078No Hit
CAACGGAATCCCAAAAGCAGCTGT228500.4974308837746753No Hit
TTCAAGTAATTCAGGATAGGTT202600.44104812714551084No Hit
ACTGGACTTGGAGTCAGAAGGC189640.4128349794268247No Hit
CCCCCACTGCTAAATTTGACTGGCTT187940.40913418072915747No Hit
CTCCCACTGCTTCACTTGACTAG184130.40084003776556226No Hit
CACCCGGCTGTGTGCACATGTGC177990.3874736236457526No Hit
GGAATACCGGGTGCTGTAGGCTT173460.37761208358667475No Hit
CACTAGATTGTGAGCTCCTGGAA160570.3495513216967161No Hit
TAGCTTATCAGACTGATGTTGACA150330.32725945189429734No Hit
CCCCCCACTGCTAAATTTGACTGGCT148700.32371103902535764No Hit
CTTCTCACTACTGCACTTGACTAG146480.31887823131428644No Hit
TATTGCACTTGTCCCGGCCTGTAG146400.318704076081455No Hit
ACCCCCCACTGCTAAATTTGACTG135390.2947359621630341No Hit
CAAAGAATTCTCCTTTTGGGCT133640.2909263164448473No Hit
TGAATACCGGGTGCTGTAGGCTTT131490.28624589456250354No Hit
GACCCGCGGGCGCTCTCCAGTCCT129590.2821097077827579No Hit
CACTAGATTGTGAGCTCCTGGA128920.280651157707795No Hit
TATTGCACTTGTCCCGGCCTGTTT128330.27936676286566337No Hit
CCCATAAAGTAGAAAGCACTAC128170.2790184524000006No Hit
CATAAAGTAGAAAGCACTACT121490.264476490458579No Hit
TTCTCACTACTGCACTTGACTAG119800.2607974611650158No Hit
CATTGCACTTGTCTCGGTCTGA118890.2588164453915587No Hit
CAACGGAATCCCAAAAGCAGCT113580.24725689181237473No Hit
CCCGCCACTGCTAAATTTGACTG112220.24429625285424098No Hit
TATTGCACTTGTCCCGGCCTGTAAT111780.2433383990736683No Hit
CTCCCACTGCTTCACTTGACTAGCCT110490.24053014594426206No Hit
CCCCCCACTGCTAAATTTGACTGG108390.2359585710824379No Hit
TATTGCACTTGTCCCGGCCTGTT105580.22984136852923512No Hit
CCCCCCACTGCTAAATTTGACTGT100500.21878251124444145No Hit
TGTAAACATCCTACACTCTCAGCT96850.21083667874650902No Hit
AGCTCGGTCTGAGGCCCCTCAGT90650.1973396482020758No Hit
CCCATAAAGTAGAAAGCACT86890.18915435225900018No Hit
CAAAGAATTCTCCTTTTGGGCTT86380.18804411264970003No Hit
TGATGTAGTAGGTTGTATAGTT83480.1817309854595619No Hit
TAGCAGCACGTAAATATTGGC82410.179401659220442No Hit
TATTGCACTTGTCCCGGCCTGTAAA78620.1711510550650546No Hit
CCCCCCACTGCTAAATTTGACTGTA74930.16311814495070645No Hit
TGAGGTAGTAGTTTGTACAGTT73840.16074527990337867No Hit
ACTGGACTTGGAGTCAGAAGGCA72120.15700094239750365No Hit
TTCAAGTAATCCAGGATAGGCTT72020.15678324835646443No Hit
AACGGAATCCCAAAAGCAGCTG71440.1555206229184368No Hit
AACATTCAACGCTGTCGGTGAGTT71400.1554335453020211No Hit
TGTAAACATCCCCGACTGGAAGC68610.14935988155702615No Hit
AACACCCTGATTGCTCCTGTCTGATT68410.14892449347494766No Hit
CCCCCCACTGCTAAATTTGACTGA68080.14820610313951815No Hit
ACTGGACTTGGAGTCAGAAGGCT67920.14785779267385535No Hit
AGCAGCATTGTACAGGGCTATGT65590.14278552151764096No Hit
TCGTACGACTCTTAGCGG64720.1408915833605995No Hit
TGAATACCGGGTGCTGTAGGCTT64600.14063035051135242No Hit
TGATGTAGTAGATTGTATAGTT63390.13799625261477755No Hit
ATGCACCTGGGCAAGGATTCTGA63370.1379527138065697No Hit
GCCCCCACTGCTAAATTTGACTG58100.1264802378438015No Hit
TATTGCACTTGTCCCGGCCTGTATT58070.1264149296314897No Hit
ACTTGACTTGGAGTCAGAAGGC56680.1233889824610442No Hit
CCTTGGCTTGGCTCTTGACGTGGACA56390.12275766974203038No Hit
TGAGGTAGTAGTTTGTGCTGTT52790.11492068426461756No Hit
CTACGGGGATGATTTTACGAACTGAA52540.11437644916201944No Hit
CCCTCCACTGCTAAATTTGACTG52260.11376690584710956No Hit
AACCGGGCGGAAACACCA52180.11359275061427816No Hit
GGAATACCGGGTGCTGTAGGCTTA51620.1123736639844584No Hit
CAACACCAGTCGATGGGCTGT50850.1106974198684562No Hit
CGCCCCACTGCTAAATTTGACTG49940.10871640409499907No Hit
TATGGCACTTGTCCCGGCCTGTAT47010.10233796869254919No Hit
TATTGCACTTGTCCCGGCCTGTTA46850.1019896582268864No Hit
ATACATGATGATCTCAATCCAAC46400.10101003504220979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)