FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9756862
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7452747.638459988467604No Hit
CAACGGAATCCCAAAAGCAGCTG6764646.933212748115121No Hit
AACATTCAACGCTGTCGGTGAGT4175284.279326693356942No Hit
AAGCTGCCAGTTGAAGAACTGT3955804.054377319265149No Hit
TAGCAGCACGTAAATATTGGCG3487883.5747968967891524No Hit
TTCAAGTAATCCAGGATAGGCT3090013.167012098767001No Hit
CCCCCACTGCTAAATTTGACTG2490112.5521627752857423No Hit
AACATTCAACGCTGTCGGTGAG2002452.052350438081424No Hit
TAGCTTATCAGACTGATGTTGAC1407031.442092754822196No Hit
TAGCTTATCAGACTGATGTTGACT1146521.1750909257505129No Hit
TATTGCACTTGTCCCGGCCTGTAA1091941.1191508089383655No Hit
TAGCTTATCAGACTGATGTTGA1045761.0718200175425254No Hit
TATTGCACTTGTCCCGGCCTGTAT1030491.0561694938393102No Hit
TATTGCACTTGTCCCGGCCTGT970780.9949715390050613No Hit
TGAGGTAGTAGGTTGTATAGTT926070.9491473795570748No Hit
CGCCTGTCTGAGCGTCGCT853820.8750969317799104No Hit
AGCAGCATTGTACAGGGCTATGA688660.705821195380236No Hit
TGTAAACATCCTTGACTGGAAGCT677920.694813557883672No Hit
CATAAAGTAGAAAGCACTACT650380.6665872695544941No Hit
GCCTGTCTGAGCGTCGCT564350.5784134284158164No Hit
TATTGCACTTGTCCCGGCCTGTA562300.5763123430463606No Hit
ACTGGACTTGGAGTCAGAAGGC507450.5200954979172606No Hit
TGAGGTAGTAGATTGTATAGTT482690.49471848633300336No Hit
AACCGGGCGGAAACACCA444600.4556792952488208No Hit
CAACGGAATCCCAAAAGCAGCTGT433220.4440157091491096No Hit
TTCAAGTAATTCAGGATAGGTT424140.4347094383419587No Hit
GGAATACCGGGTGCTGTAGGCTTT405010.4151027246260119No Hit
CCCATAAAGTAGAAAGCACTAC402700.4127351601365275No Hit
TGTAAACATCCCCGACTGGAAGCT401620.41162824686871663No Hit
TCGTACGACTCTTAGCGG398920.40886096369918934No Hit
CATTGCACTTGTCTCGGTCTGA342890.3514347133330368No Hit
TGATGTAGTAGGTTGTATAGTT308710.3164029582462066No Hit
AACATTCAACGCTGTCGGTGAGTT297520.3049341068880548No Hit
CCCATAAAGTAGAAAGCACT294260.30159286869077373No Hit
CTACGCCTGTCTGAGCGTCGCT291270.2985283588104454No Hit
GGAATACCGGGTGCTGTAGGCTT286050.2931782780160261No Hit
TGTAAACATCCTACACTCTCAGCT263360.269922850195073No Hit
GCGTTCGCGCTTTCCCCTG257340.26375283364671964No Hit
CACCCGGCTGTGTGCACATGTGC254620.260965052083344No Hit
TTCTCACTACTGCACTTGACTAG252510.2588024715323431No Hit
TACGCCTGTCTGAGCGTCGCT245070.25117706902075687No Hit
TGAGGTAGTAGTTTGTACAGTT244940.2510438294607426No Hit
TCCTGTCTGAGCGTCGCT237000.24290596710294768No Hit
ACGCCTGTCTGAGCGTCGCT234200.24003619196417864No Hit
CTCCCACTGCTTCACTTGACTAG228330.23401991336968794No Hit
CTTCTCACTACTGCACTTGACTAG223720.22929503358764322No Hit
TTAATGCTAATCGTGATAGGGGTT220890.22639451085810172No Hit
TGTCAGTTTGTCAAATACCCCAA214420.2197632804481605No Hit
CAACACCAGTCGATGGGCTGT213630.21895359389115066No Hit
TTCAAGTAATCCAGGATAGGCTT199810.2047892037419408No Hit
ACTTGACTTGGAGTCAGAAGGC192410.1972047980180513No Hit
TGAGGTAGTAGTTTGTGCTGTT184850.189456405143375No Hit
GACCCGCGGGCGCTCTCCAGTCCT182060.18659687920153017No Hit
CAACGGAATCCCAAAAGCAGCT179940.18442404945360505No Hit
TGAATACCGGGTGCTGTAGGCTTT179430.18390134041047212No Hit
TAGCAGCACGTAAATATTGGC176400.18079583374244712No Hit
TAGCTTATCAGACTGATGTTGACA175830.18021162951776915No Hit
TGATGTAGTAGATTGTATAGTT162190.16623172491319443No Hit
CCCCCCACTGCTAAATTTGACTGGCT158920.1628802375189892No Hit
CCCCCCACTGCTAAATTTGACTGG157980.16191681300811675No Hit
TATTGCACTTGTCCCGGCCTGTT156960.1608713949218509No Hit
AACATTCAACGCTGTCGGTGA156760.1606664109833674No Hit
TGCAAGTAATCCAGGATAGGCT153510.1573354219830105No Hit
TTCAAGTAATCCAGGATAGGC150460.15420941692113715No Hit
AGCTCGGTCTGAGGCCCCTCAGT150050.15378919984724596No Hit
CCCCCACTGCTAAATTTGACTGGCTT147750.1514318845546857No Hit
ACCACAGGGTAGAACCACGGAC144700.14830587949281232No Hit
AGCAGCATTGTACAGGGCTATGT144320.1479164100096937No Hit
CACTAGATTGTGAGCTCCTGGA140830.1443394402831566No Hit
ATCCCACCGCTGCCACCA136640.1400450267719273No Hit
TAGCAGCACGTAAATATTGGCGT135150.13851789643022522No Hit
TGTAAACATCCCCGACTGGAAGC135030.13839490606713512No Hit
CAAAGAATTCTCCTTTTGGGCTT131770.13505366786985407No Hit
AACGGAATCCCAAAAGCAGCTG131390.1346641983867354No Hit
CAAAGAATTCTCCTTTTGGGCT130830.13409024335898162No Hit
TATTGCACTTGTCCCGGCCTGTAG130000.13323956001427506No Hit
ACTGGACTTGGAGTCAGAAGGCA128940.13215314514031254No Hit
ATCCCGGACGAGCCCCCA127810.13099498588788075No Hit
TGTAAACATCCTACACTCAGCT125590.1287196641707139No Hit
TGAATACCGGGTGCTGTAGGCTT125370.12849418183838204No Hit
AACATTCAACGCTGTCGGTGAGA125370.12849418183838204No Hit
TGAGGTAGTAGGTTGTGTGGTT123450.12652633602894045No Hit
TGTCAGTTTGTCAAATACCCCA123040.12610611895504928No Hit
TTATAAAGCAATGAGACTGATT120360.12335933417937038No Hit
TCGTTCGCGCTTTCCCCTG120340.12333883578552203No Hit
CAAAGTGCTGTTCGTGCAGGT118780.12173996106535072No Hit
AGCAGCATTGTACAGGGCTATG118020.12096102209911343No Hit
CGCCCCACTGCTAAATTTGACTG109910.11264892339360749No Hit
ACTGGACTTGGAGTCAGAAGGCT109630.11236194587973058No Hit
TTCACAGTGGCTAAGTTCCG108300.11099880268881533No Hit
CCCGCCACTGCTAAATTTGACTG106980.10964590869482421No Hit
CACTAGATTGTGAGCTCCTGGAA105550.10818027353466719No Hit
TTCACAGTGGCTAAGTTCTGC104310.10690937311606949No Hit
ACTGGACTTGGAGTCAGAAGG103830.10641741166370908No Hit
CTCCCACTGCTTCACTTGACTAGCCT101370.10389610922036203No Hit
CTCCCCACTGCTAAATTTGACTG99590.1020717521678589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)