FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8356715
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6233087.4587681882174985No Hit
CCCCCCACTGCTAAATTTGACTG5208916.233202879361089No Hit
GCCTGTCTGAGCGTCGCT4814605.761354790728175No Hit
TTCAAGTAATCCAGGATAGGCT2681213.208449731742676No Hit
AACATTCAACGCTGTCGGTGAGT2652283.1738308653579788No Hit
AAGCTGCCAGTTGAAGAACTGT2650643.1718683717226206No Hit
TAGCAGCACGTAAATATTGGCG2420392.896341445173133No Hit
TCCTGTCTGAGCGTCGCT1803422.1580489462665655No Hit
CCCCCACTGCTAAATTTGACTG1757772.1034222179409015No Hit
AACATTCAACGCTGTCGGTGAG1422881.7026786243158942No Hit
TAGCTTATCAGACTGATGTTGAC1166961.3964338858032133No Hit
TAGCTTATCAGACTGATGTTGA841751.0072737911966605No Hit
TAGCTTATCAGACTGATGTTGACT817870.9786979692379122No Hit
TGAGGTAGTAGGTTGTATAGTT776700.9294321991356651No Hit
CGCCTGTCTGAGCGTCGCT712550.8526675852891956No Hit
TATTGCACTTGTCCCGGCCTGTAA690420.8261858876364696No Hit
TATTGCACTTGTCCCGGCCTGTAT642960.7693932364571485No Hit
TATTGCACTTGTCCCGGCCTGT638760.7643673381226954No Hit
CATAAAGTAGAAAGCACTACT625000.7479015378650583No Hit
AGCAGCATTGTACAGGGCTATGA503680.6027248745469961No Hit
TGTAAACATCCTTGACTGGAAGCT471090.5637262967565605No Hit
TGAGGTAGTAGATTGTATAGTT437120.5230763523705187No Hit
CCCATAAAGTAGAAAGCACTAC402450.4815887582620683No Hit
TATTGCACTTGTCCCGGCCTGTA392300.46944283728713976No Hit
CAACGGAATCCCAAAAGCAGCTGT378190.45255821216829817No Hit
TTCAAGTAATTCAGGATAGGTT343830.4114415772226288No Hit
CTACGCCTGTCTGAGCGTCGCT343600.41116634945669445No Hit
ACTGGACTTGGAGTCAGAAGGC332080.39738102831076566No Hit
TGTAAACATCCCCGACTGGAAGCT297600.3561207962698261No Hit
CTCCCACTGCTTCACTTGACTAG271240.3245773010088294No Hit
TTCTCACTACTGCACTTGACTAG263610.3154469190345728No Hit
CCCCCACTGCTAAATTTGACTGGCTT250780.3000939962652789No Hit
CATTGCACTTGTCTCGGTCTGA240370.28763694825059843No Hit
CCCATAAAGTAGAAAGCACT231210.27667570331164815No Hit
TACGCCTGTCTGAGCGTCGCT229060.27410292202139236No Hit
ACGCCTGTCTGAGCGTCGCT218360.2612988476931426No Hit
AACATTCAACGCTGTCGGTGAGTT217720.26053299651836875No Hit
CTTCTCACTACTGCACTTGACTAG214830.2570746998072807No Hit
TGTAAACATCCTACACTCTCAGCT211130.25264712270311956No Hit
TCCACCACGTTCCCGTGG205520.24593395849924282No Hit
CCCCCCACTGCTAAATTTGACTGGCT204180.24433045760206012No Hit
TGAGGTAGTAGTTTGTACAGTT201150.24070463094649033No Hit
CAACACCAGTCGATGGGCTGT185160.22157031800175067No Hit
CCCTGTCTGAGCGTCGCT182320.21817185341369189No Hit
TCGTACGACTCTTAGCGG174480.20879017652271256No Hit
CACCCGGCTGTGTGCACATGTGC173940.20814398959399716No Hit
TAGCTTATCAGACTGATGTTGACA169480.2028069642197921No Hit
CCCCCCACTGCTAAATTTGACTGG162350.19427490347582752No Hit
TTCACAGTGGCTAAGTTCCG155060.18555137993816948No Hit
TGAGGTAGTAGTTTGTGCTGTT154990.1854676149659286No Hit
TGTCAGTTTGTCAAATACCCCAA152780.18282303512803774No Hit
ATCCCACCGCTGCCACCA150310.17986732825039503No Hit
GCGTTCGCGCTTTCCCCTG149270.17862282009138758No Hit
AACCGGGCGGAAACACCA142800.17088054337140848No Hit
TAGCAGCACGTAAATATTGGC140710.16837956062878773No Hit
TTCAAGTAATCCAGGATAGGCTT140620.16827186280733517No Hit
GTCAGGATGGCCGAGCGGTCTAAGG136270.16306646810379438No Hit
AGCTCGGTCTGAGGCCCCTCAGT135060.1616185307264876No Hit
TTCAAGTAATCCAGGATAGGC131420.15726275216996152No Hit
GACCCGCGGGCGCTCTCCAGTCCT129880.15541992278066202No Hit
TGATGTAGTAGGTTGTATAGTT129420.15486946724879333No Hit
AACGGAATCCCAAAAGCAGCTG128000.15317023495476392No Hit
TAGCAGCACATCATGGTTTACA127360.1524043837799901No Hit
TAGCAGCACGTAAATATTGGCGT125770.1505017222676614No Hit
TGTCAGTTTGTCAAATACCCCA125760.15048975584305554No Hit
TCAGGCTCAGTCCCCTCCCGAT122630.14674426494142734No Hit
TTCACAGTGGCTAAGTTCCGC122480.14656476857233974No Hit
TGTAAACATCCTACACTCAGCT114710.13726685665360133No Hit
TATTGCACTTGTCCCGGCCTGTT111960.13397608988699505No Hit
CTCCCACTGCTTCACTTGACTAGCCT111520.13344956720433807No Hit
CAAAGAATTCTCCTTTTGGGCT110160.1318221334579437No Hit
ATCACATTGCCAGGGATTTCC109530.13106824870777573No Hit
TGAGGTAGTAGGTTGTGTGGTT108260.1295485127828339No Hit
TGTAAACATCCCCGACTGGAAGC101930.12197376600733663No Hit
GGAATACCGGGTGCTGTAGGCTTT101520.12148314259849714No Hit
AGCAGCATTGTACAGGGCTATGT100420.12016683589185463No Hit
CAACGGAATCCCAAAAGCAGCT100110.11979587672907356No Hit
ACCACAGGGTAGAACCACGGAC97290.11642134499022642No Hit
TTCACAGTGGCTAAGTTCTGC95900.11475801197001452No Hit
TAGCAGCACATAATGGTTTGT94340.11289124973150334No Hit
AGCAGCATTGTACAGGGCTATG93830.11228096207660547No Hit
CAAAGTGCTGTTCGTGCAGGT92820.11107235319141552No Hit
ATCCCGGACGAGCCCCCA91950.11003127425070736No Hit
TTATAAAGCAATGAGACTGATT91410.10938508732199195No Hit
GGGCTACGCCTGTCTGAGCGTCGCT90230.10797304921850272No Hit
TCCCACTGCTTCACTTGACTAG89710.107350795138999No Hit
ACCCCCCACTGCTAAATTTGACTG88880.1063575818967142No Hit
TCCGGTCTGAGCGTCGCT86940.10403609552318106No Hit
TACCTGGTTGATCCTGCC84180.10073336233196897No Hit
TAGTGGGTTATCAGAACT84060.10058976523669887No Hit
ACTGGACTTGGAGTCAGAAGG83810.10029060462155284No Hit
CAAAGAATTCTCCTTTTGGGCTT83630.1000752089786477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)