FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5979247
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG88605514.818839228417893No Hit
CAACGGAATCCCAAAAGCAGCTG5834399.757733707940147No Hit
CCCCCACTGCTAAATTTGACTG2116173.539191473441388No Hit
AACATTCAACGCTGTCGGTGAGT1725252.885396773205723No Hit
TTCAAGTAATCCAGGATAGGCT1676692.804182533352444No Hit
TATTGCACTTGTCCCGGCCTGTAT1610002.6926467496659696No Hit
AACATTCAACGCTGTCGGTGAG1379332.30686238584892No Hit
TAGCAGCACGTAAATATTGGCG1344542.2486778017365734No Hit
AAGCTGCCAGTTGAAGAACTGT1307142.1861281194772517No Hit
TATTGCACTTGTCCCGGCCTGTAA1263232.112690778621455No Hit
TAGCTTATCAGACTGATGTTGACT1233052.062216195450698No Hit
GCCTGTCTGAGCGTCGCT1206322.0175115696006536No Hit
TAGCTTATCAGACTGATGTTGAC998741.6703441085474475No Hit
TGAGGTAGTAGGTTGTATAGTT730931.222444899834377No Hit
TAGCTTATCAGACTGATGTTGA647871.08353108677397No Hit
TATTGCACTTGTCCCGGCCTGT641541.07294446942901No Hit
TATTGCACTTGTCCCGGCCTGTA565950.946523868306494No Hit
TGTAAACATCCTTGACTGGAAGCT507270.8483844203124573No Hit
TGAGGTAGTAGATTGTATAGTT472520.7902667342560024No Hit
TGTAAACATCCCCGACTGGAAGCT433480.724974231705096No Hit
AGCAGCATTGTACAGGGCTATGA430620.7201910207087949No Hit
CAACGGAATCCCAAAAGCAGCTGT343160.5739184215002324No Hit
ACTGGACTTGGAGTCAGAAGGC307610.514462774325931No Hit
TTCAAGTAATTCAGGATAGGTT304340.5089938582567337No Hit
GGAATACCGGGTGCTGTAGGCTTT299260.5004978051584087No Hit
CACTAGATTGTGAGCTCCTGGAA214500.35874082472257796No Hit
CATAAAGTAGAAAGCACTACT192590.3220974146075585No Hit
ACCCCCCACTGCTAAATTTGACTG192050.3211942908530121No Hit
CATTGCACTTGTCTCGGTCTGA190490.3185852666732115No Hit
CCCATAAAGTAGAAAGCACTAC189190.316411079856711No Hit
TAGCTTATCAGACTGATGTTGACA187020.3127818603245526No Hit
TCGTACGACTCTTAGCGG185930.31095888830148677No Hit
CACTAGATTGTGAGCTCCTGGA182840.30579101348380494No Hit
TATTGCACTTGTCCCGGCCTGTTT175080.2928127906406944No Hit
TATTGCACTTGTCCCGGCCTGTAG168930.2825272145472498No Hit
CAAAGAATTCTCCTTTTGGGCT167910.2808213141219956No Hit
TATTGCACTTGTCCCGGCCTGTT167300.2798011187696377No Hit
TGTAAACATCCTACACTCTCAGCT165470.27674053271256394No Hit
GGAATACCGGGTGCTGTAGGCTT162590.2719238726883168No Hit
TGAGGTAGTAGTTTGTACAGTT162420.2716395559507744No Hit
CAACGGAATCCCAAAAGCAGCT160230.26797688739066977No Hit
CCCCCACTGCTAAATTTGACTGGCTT158430.2649664748755152No Hit
AGCTCGGTCTGAGGCCCCTCAGT158000.2642473207746728No Hit
AACGGAATCCCAAAAGCAGCTG144480.2416357778830679No Hit
TATTGCACTTGTCCCGGCCTGTAAT143530.24004694905562524No Hit
CCCCCCACTGCTAAATTTGACTGT139040.2325376422817121No Hit
CTCCCACTGCTTCACTTGACTAG132610.22178377979702127No Hit
AACATTCAACGCTGTCGGTGAGTT122120.20423976463925975No Hit
CCCATAAAGTAGAAAGCACT119850.20044329996737048No Hit
CCCCCCACTGCTAAATTTGACTGGCT115090.19248243131618412No Hit
TATTGCACTTGTCCCGGCCTGTAAA114540.1915625830476647No Hit
CCCCCCACTGCTAAATTTGACTGG114440.1913953379079339No Hit
CTTCTCACTACTGCACTTGACTAG104910.17545687609158814No Hit
TAGCAGCACGTAAATATTGGC101150.16916845883770984No Hit
TTCAAGTAATCCAGGATAGGCTT100780.16854965182070586No Hit
CAACACCAGTCGATGGGCTGT100680.16838240668097504No Hit
ACTGGACTTGGAGTCAGAAGGCA99800.16691064945134396No Hit
TTCTCACTACTGCACTTGACTAG98910.1654221677077398No Hit
TGTAAACATCCCCGACTGGAAGC98280.1643685233274357No Hit
CACCCGGCTGTGTGCACATGTGC97870.1636828182545394No Hit
CAAAGAATTCTCCTTTTGGGCTT97100.1623950306786122No Hit
TGAGGTAGTAGTTTGTGCTGTT95300.1593846181634577No Hit
AGCAGCATTGTACAGGGCTATGT89190.14916594012590548No Hit
ACTGGACTTGGAGTCAGAAGGCT89020.1488816233883631No Hit
CCCCCCACTGCTAAATTTGACTGTA79910.13364559115888675No Hit
CCCCCCACTGCTAAATTTGACTGA79000.1321236603873364No Hit
AACCGGGCGGAAACACCA77500.1296149832913743No Hit
TATTGCACTTGTCCCGGCCTGTATT74290.12424641430601546No Hit
ATCCCGGACGAGCCCCCA70460.1178409254543256No Hit
ACCGGGTGCTGTAGGCTTA70290.11755660871678322No Hit
ACCGGGTGCTGTAGGCTT64650.10812398283596579No Hit
ATGCACCTGGGCAAGGATTCTGA64310.10755534936088107No Hit
TATTGCACTTGTCCCGGCCTGTTA64230.10742155324909641No Hit
TCGTTTCCCGGGCGGCGCACCA64040.1071037874836079No Hit
TGAGGTAGTAGGTTGTGTGGTT63810.10671912366222702No Hit
TATTGCACTTGTCCCGGCCTGA62830.10508012129286513No Hit
TTCAAGTAATCCAGGATAGGC62390.1043442426780496No Hit
CTCCCACTGCTTCACTTGACTAGCCT62220.10405992594050723No Hit
CGCGACCTCAGATCAGACGTGGCG61850.10344111892350324No Hit
TGAGGGGCAGAGAGCGAGACTTT60860.10178539204016826No Hit
TGAGGTAGTAGGTTGTATAGT60330.100898992799595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)