FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10680634
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG113050810.584652559014755No Hit
CAACGGAATCCCAAAAGCAGCTG7085346.633819677745721No Hit
AAGCTGCCAGTTGAAGAACTGT5047384.725730701005203No Hit
GCCTGTCTGAGCGTCGCT4255003.983845902780678No Hit
AACATTCAACGCTGTCGGTGAGT4091403.8306714751202975No Hit
TTCAAGTAATCCAGGATAGGCT3142962.9426717552534805No Hit
TAGCAGCACGTAAATATTGGCG3092792.8956988882869688No Hit
CCCCCACTGCTAAATTTGACTG2949512.761549548463134No Hit
TCCACCACGTTCCCGTGG2227612.08565334230159No Hit
AACATTCAACGCTGTCGGTGAG1909411.7877309530501655No Hit
TGAGGTAGTAGGTTGTATAGTT1394731.3058494467650517No Hit
TAGCTTATCAGACTGATGTTGAC1303721.2206391493239073No Hit
CGCCTGTCTGAGCGTCGCT999340.9356560668589524No Hit
TAGCTTATCAGACTGATGTTGACT981520.9189716640416665No Hit
TAGCTTATCAGACTGATGTTGA950890.8902935911856917No Hit
TATTGCACTTGTCCCGGCCTGT850130.7959546221694331No Hit
TATTGCACTTGTCCCGGCCTGTAA770210.7211276034737263No Hit
TATTGCACTTGTCCCGGCCTGTAT725650.6794072336904344No Hit
TCCTGTCTGAGCGTCGCT725100.6788922829861973No Hit
AGCAGCATTGTACAGGGCTATGA684530.6409076464936445No Hit
CATAAAGTAGAAAGCACTACT672620.6297566230618894No Hit
TGAGGTAGTAGATTGTATAGTT601250.5629347471320523No Hit
TGTAAACATCCTTGACTGGAAGCT540730.5062714441858039No Hit
TAGCAGCACATCATGGTTTACA533890.49986732997310834No Hit
GGAATACCGGGTGCTGTAGGCTTT515770.4829020449535112No Hit
ACTGGACTTGGAGTCAGAAGGC456360.42727800615581435No Hit
TTCACAGTGGCTAAGTTCCGC454030.4250964877178639No Hit
CAACGGAATCCCAAAAGCAGCTGT451710.4229243320199905No Hit
CCCATAAAGTAGAAAGCACTAC424980.39789772779406163No Hit
GGAATACCGGGTGCTGTAGGCTT416890.390323271071736No Hit
TATTGCACTTGTCCCGGCCTGTA408140.3821308735043257No Hit
CTACGCCTGTCTGAGCGTCGCT391230.3662984800340504No Hit
TACGCCTGTCTGAGCGTCGCT389870.3650251473835729No Hit
TGTAAACATCCCCGACTGGAAGCT374940.35104657644855164No Hit
TCAGGCTCAGTCCCCTCCCGAT348140.32595443304208344No Hit
AACCGGGCGGAAACACCA322160.3016300343219326No Hit
ACCGGGTGCTGTAGGCTT320810.30036606441153213No Hit
TGTAAACATCCTACACTCTCAGCT319580.29921444738205616No Hit
TTCAAGTAATTCAGGATAGGTT318600.2982968988545062No Hit
TGAGGTAGTAGTTTGTACAGTT310890.2910782262551081No Hit
AACATTCAACGCTGTCGGTGAGTT299960.280844751350903No Hit
CATTGCACTTGTCTCGGTCTGA297660.2786913211331837No Hit
ACGCCTGTCTGAGCGTCGCT288390.27001206108176723No Hit
TCGTACGACTCTTAGCGG280780.2628870158831395No Hit
TCAGGCTCAGTCCCCTCCCGA255840.23953634213100083No Hit
TCGTTTCCCGGCCAATGCACCA254170.23797276453813512No Hit
CCCCCCACTGCTAAATTTGACTGG244850.22924669078633347No Hit
GCGTTCGCGCTTTCCCCTG240650.22531433995397648No Hit
CCCATAAAGTAGAAAGCACT231280.2165414525017897No Hit
CAACACCAGTCGATGGGCTGT224780.21045567145171343No Hit
TGAGGTAGTAGTTTGTGCTGTT220560.20650459513920238No Hit
TTAATGCTAATCGTGATAGGGGTT217360.20350851831454947No Hit
TGAGGTAGTAGGTTGTGTGGTT202700.1897827413616083No Hit
CCCCCACTGCTAAATTTGACTGGCTT199920.1871798996201911No Hit
GGGCTACGCCTGTCTGAGCGTCGCT199460.18674921357664723No Hit
GTCAGGATGGCCGAGCGGTCTAAGG191290.17909985493370525No Hit
ATCACATTGCCAGGGATTTCC190080.17796696338438336No Hit
ACCGGGTGCTGTAGGCTTT187890.17591652330751154No Hit
AACGGAATCCCAAAAGCAGCTG186680.17478363175818964No Hit
AGCTCGGTCTGAGGCCCCTCAGT185480.1736601029489448No Hit
TTCACAGTGGCTAAGTTCCG173620.1625558932175749No Hit
CCCCCCACTGCTAAATTTGACTGGCT157880.14781894033631338No Hit
ACCCCCCACTGCTAAATTTGACTG153620.14383041306349417No Hit
TAGCAGCACGTAAATATTGGCGT151740.1420702179290106No Hit
TAGCTTATCAGACTGATGTTGACA151330.14168634558585194No Hit
CTCCCACTGCTTCACTTGACTAG149860.14031002279452698No Hit
CGCGACCTCAGATCAGACGTGGCG147620.13821276901726995No Hit
TGTAAACATCCTACACTCAGCT146580.13723904404925774No Hit
CAACGGAATCCCAAAAGCAGCT144760.1355350253552364No Hit
TTCTCACTACTGCACTTGACTAG144220.13502943739107623No Hit
TAGCAGCACGTAAATATTGGC143290.13415870256391146No Hit
TGAGGTAGTAGGTTGTATAGT141940.132894732653511No Hit
TTCAAGTAATCCAGGATAGGCTT140170.13123752765987487No Hit
TTCAAGTAATCCAGGATAGGC140000.1310783610785652No Hit
CGCGACCTCAGATCAGACGTGG138510.12968331280708617No Hit
TATTGCACTTGTCCCGGCCTGTT137030.1282976272756842No Hit
ATCCCGGACGAGCCCCCA135490.12685576530381998No Hit
AACATTCAACGCTGTCGGTGA134890.12629400089919754No Hit
AGCAGCATTGTACAGGGCTATGT134370.12580713841519148No Hit
ACCGGGTGCTGTAGGCTTA133010.12453380576471396No Hit
AACATTCAACGCTGTCGGTGAGA132060.12364434545739512No Hit
CTTCTCACTACTGCACTTGACTAG131430.12305449283254159No Hit
ACCACAGGGTAGAACCACGGAC130070.12178116018206409No Hit
AGAGGTAGTAGGTTGCATAGTT128610.12041420013081619No Hit
TTCACAGTGGCTAAGTTCTGC126810.11872890691694894No Hit
GCCCCCACTGCTAAATTTGACTG126130.11809224059171018No Hit
ATCACATTGCCAGGGATTTCCA125590.11758665262755003No Hit
AGCAGCATTGTACAGGGCTATG124090.11618224161599394No Hit
TAGCAGCACATAATGGTTTGT123990.11608861421522355No Hit
ACTGGACTTGGAGTCAGAAGGCA117540.11004964686553251No Hit
CAAAGTGCTGTTCGTGCAGGT115010.1076808736260413No Hit
CCCCCCACTGCTAAATTTGACTGT112720.10553680614839905No Hit
TACCTGGTTGATCCTGCC112710.10552744340832203No Hit
CAAAGAATTCTCCTTTTGGGCT109850.10284969974628846No Hit
TCGTTTCCCGGCCAACGCACCA107010.10019068156440901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)