FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7392068
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5601347.577500639875066No Hit
CCCCCCACTGCTAAATTTGACTG4579856.195627529400434No Hit
GCCTGTCTGAGCGTCGCT4491206.075701684562426No Hit
AAGCTGCCAGTTGAAGAACTGT3602194.873047704647739No Hit
AACATTCAACGCTGTCGGTGAGT2388473.2311255794724834No Hit
TAGCAGCACGTAAATATTGGCG2106252.849337966046849No Hit
TTCAAGTAATCCAGGATAGGCT2100452.8414917178792187No Hit
TCCTGTCTGAGCGTCGCT1627912.2022389404426477No Hit
CCCCCACTGCTAAATTTGACTG1338441.8106435168074753No Hit
AACATTCAACGCTGTCGGTGAG1191931.6124445824903126No Hit
TAGCTTATCAGACTGATGTTGAC1036981.4028280042878394No Hit
TAGCTTATCAGACTGATGTTGACT741661.003318692414626No Hit
TGAGGTAGTAGGTTGTATAGTT711680.9627617061964256No Hit
TATTGCACTTGTCCCGGCCTGT673100.9105706278676008No Hit
TATTGCACTTGTCCCGGCCTGTAA664990.8995994084470003No Hit
TAGCTTATCAGACTGATGTTGA660690.893782362391688No Hit
TATTGCACTTGTCCCGGCCTGTAT626830.8479765067096243No Hit
TGTAAACATCCTTGACTGGAAGCT580450.7852335773967447No Hit
AGCAGCATTGTACAGGGCTATGA511440.691876752216024No Hit
GGAATACCGGGTGCTGTAGGCTTT496220.671287114783035No Hit
CGCCTGTCTGAGCGTCGCT492050.6656459329107903No Hit
CATAAAGTAGAAAGCACTACT441970.5978976383875256No Hit
CAACGGAATCCCAAAAGCAGCTGT399220.5400653781864561No Hit
TGTAAACATCCCCGACTGGAAGCT392640.5311639449204201No Hit
CCCATAAAGTAGAAAGCACTAC388680.5258068513439No Hit
GGAATACCGGGTGCTGTAGGCTT359620.4864944424212547No Hit
TGAGGTAGTAGATTGTATAGTT357330.48339652719644893No Hit
TATTGCACTTGTCCCGGCCTGTA336870.4557182103844283No Hit
AACCGGGCGGAAACACCA322260.4359537818104487No Hit
TTAATGCTAATCGTGATAGGGGTT298200.4034053799288643No Hit
TTCAAGTAATTCAGGATAGGTT270740.36625745325935855No Hit
TGTAAACATCCTACACTCTCAGCT268350.3630242578937315No Hit
ACTGGACTTGGAGTCAGAAGGC259340.35083551720573997No Hit
TGTCAGTTTGTCAAATACCCCAA219300.2966693488209254No Hit
TCGTACGACTCTTAGCGG219090.2963852605252008No Hit
CTACGCCTGTCTGAGCGTCGCT212920.2880384758365318No Hit
TGAATACCGGGTGCTGTAGGCTTT208920.28262727020368317No Hit
CATTGCACTTGTCTCGGTCTGA208560.28214026169672685No Hit
AACATTCAACGCTGTCGGTGAGTT193700.2620376327706942No Hit
TGATGTAGTAGGTTGTATAGTT181360.2453440633933562No Hit
TGAGGTAGTAGTTTGTACAGTT167370.22641837169246817No Hit
CCCATAAAGTAGAAAGCACT153480.2076279601324014No Hit
TGAATACCGGGTGCTGTAGGCTT152260.20597754241438254No Hit
TCCACCACGTTCCCGTGG151580.20505763745679828No Hit
TACGCCTGTCTGAGCGTCGCT150820.20402950838655703No Hit
TGTCAGTTTGTCAAATACCCCA140400.1899333177129864No Hit
TTCACAGTGGCTAAGTTCCGC139180.18828289999496758No Hit
TAGCTTATCAGACTGATGTTGACA137030.18537437696731143No Hit
ACGCCTGTCTGAGCGTCGCT134230.18158653302431743No Hit
CAACACCAGTCGATGGGCTGT129650.17539070257470576No Hit
TTCTCACTACTGCACTTGACTAG128710.17411906925098633No Hit
ACCCCCCACTGCTAAATTTGACTG128400.17369970081444056No Hit
TAGCAGCACATCATGGTTTACA126830.17157580260354746No Hit
ACCGGGTGCTGTAGGCTT126790.171521690547219No Hit
TGAGGTAGTAGTTTGTGCTGTT126010.1704665054488135No Hit
CCCCCACTGCTAAATTTGACTGGCTT125540.1698306887869538No Hit
CAAAGAATTCTCCTTTTGGGCT124260.16809910298444225No Hit
GCGTTCGCGCTTTCCCCTG124250.1680855749703601No Hit
TGTAAACATCCCCGACTGGAAGC123030.1664351572523413No Hit
TGTAAACATCCTACACTCAGCT122360.16552878030883914No Hit
TAGCAGCACGTAAATATTGGC120690.16326960195712487No Hit
CCCCCCACTGCTAAATTTGACTGG116470.15756078001446958No Hit
CAGGTCCCTGTTCGGGCGCCA114530.154936345282538No Hit
AACGGAATCCCAAAAGCAGCTG110900.15002567617072785No Hit
CTTCTCACTACTGCACTTGACTAG110820.1499174520580709No Hit
TAGCAGCACGTAAATATTGGCGT110790.14987686801582453No Hit
TATTGCACTTGTCCCGGCCTGTT110330.14925457936804692No Hit
TCGTTTCCCGGCCAATGCACCA108550.14684659286142931No Hit
TTCACAGTGGCTAAGTTCCG106270.1437622056507056No Hit
TTCAAGTAATCCAGGATAGGCTT103160.1395549932711658No Hit
ATCACATTGCCAGGGATTTCC102430.13856744824317094No Hit
ATCCCGGACGAGCCCCCA102150.13818866384887152No Hit
TCAGGCTCAGTCCCCTCCCGAT101200.13690350251106997No Hit
GGGCTACGCCTGTCTGAGCGTCGCT100000.13528014082121537No Hit
CAACGGAATCCCAAAAGCAGCT99450.13453610004669872No Hit
AGCTCGGTCTGAGGCCCCTCAGT97660.13211458552599895No Hit
TTCAAGTAATCCAGGATAGGC97140.13141112879372863No Hit
CTCCCACTGCTTCACTTGACTAG96870.13104587241351134No Hit
ATCCCACCGCTGCCACCA94860.12832674158300492No Hit
CAAAGAATTCTCCTTTTGGGCTT94600.12797501321686974No Hit
ATCACATTGCCAGGGATTTCCA92010.12447125756960029No Hit
TGATGTAGTAGATTGTATAGTT91950.12439008948510755No Hit
AGCAGCATTGTACAGGGCTATGT89890.1216033185841905No Hit
TGAGGTAGTAGGTTGTGTGGTT88790.12011523703515715No Hit
ACCACAGGGTAGAACCACGGAC86300.11674676152870889No Hit
AGCAGCATTGTACAGGGCTATG84760.11466344736006218No Hit
CCCTGTCTGAGCGTCGCT84200.11390587857146336No Hit
CCCCCCACTGCTAAATTTGACTGGCT84120.11379765445880638No Hit
GTCAGGATGGCCGAGCGGTCTAAGG81480.11022625874112629No Hit
ACCGGGTGCTGTAGGCTTT81030.10961749810743082No Hit
TGAGAACTGAATTCCATAGGCTGT79680.10779121620634441No Hit
CACTAGATTGTGAGCTCCTGGA79330.10731773571347016No Hit
AACATTCAACGCTGTCGGTGA78560.10627607862914681No Hit
CAAAGTGCTGTTCGTGCAGGTAG78030.10555909388279437No Hit
ACTGGACTTGGAGTCAGAAGGCA77360.10465271693929222No Hit
AACATTCAACGCTGTCGGTGAGA76070.10290760312269855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)