FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8207366
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG101893212.414847833031937No Hit
CAACGGAATCCCAAAAGCAGCTG7347668.952518993304308No Hit
CCCCCACTGCTAAATTTGACTG3529754.3007098745200345No Hit
AACATTCAACGCTGTCGGTGAGT2782973.390819904948798No Hit
AACATTCAACGCTGTCGGTGAG2304262.807551168060496No Hit
AAGCTGCCAGTTGAAGAACTGT2025792.4682583913036167No Hit
GCCTGTCTGAGCGTCGCT2019182.460204650310465No Hit
TTCAAGTAATCCAGGATAGGCT1632651.9892496569544966No Hit
TAGCAGCACGTAAATATTGGCG1449791.766449796438955No Hit
TATTGCACTTGTCCCGGCCTGTAA1391621.6955744388638208No Hit
TATTGCACTTGTCCCGGCCTGTAT1245061.5170031408371456No Hit
GGAATACCGGGTGCTGTAGGCTTT1182381.440632719437637No Hit
TAGCTTATCAGACTGATGTTGACT1176211.433115082232229No Hit
TAGCTTATCAGACTGATGTTGAC1090831.3290865790559359No Hit
TGAGGTAGTAGGTTGTATAGTT997071.2148477355585214No Hit
TAGCTTATCAGACTGATGTTGA909281.1078828457266314No Hit
TATTGCACTTGTCCCGGCCTGT795530.9692878324178549No Hit
GGAATACCGGGTGCTGTAGGCTT765840.9331130109221399No Hit
TATTGCACTTGTCCCGGCCTGTA650820.7929706071351027No Hit
TCCTGTCTGAGCGTCGCT574090.6994814170587738No Hit
TGAGGTAGTAGATTGTATAGTT466050.5678435688136729No Hit
TCGTACGACTCTTAGCGG439890.5359697617969029No Hit
AGCAGCATTGTACAGGGCTATGA425880.5188997298280593No Hit
TGTAAACATCCCCGACTGGAAGCT378920.4616828346633987No Hit
TTAATGCTAATCGTGATAGGGGTT368890.449462105138238No Hit
TGTAAACATCCTTGACTGGAAGCT356730.43464614591331735No Hit
CAACGGAATCCCAAAAGCAGCTGT348950.42516685621184674No Hit
TGAATACCGGGTGCTGTAGGCTTT347640.42357072902560944No Hit
CAACACCAGTCGATGGGCTGT316150.3852027556709424No Hit
ACTGGACTTGGAGTCAGAAGGC312320.3805362158821722No Hit
CATTGCACTTGTCTCGGTCTGA301930.3678768559852211No Hit
CAACGGAATCCCAAAAGCAGCT301840.3677671983922735No Hit
TGAGAACTGAATTCCATAGGCTGT293940.3581416985668727No Hit
CATAAAGTAGAAAGCACTACT286520.34910103923719255No Hit
TTCAAGTAATTCAGGATAGGTT265780.32383105615126706No Hit
TGATGTAGTAGGTTGTATAGTT228920.27892017975072636No Hit
TGAATACCGGGTGCTGTAGGCTT225570.27483848045767667No Hit
CACTAGATTGTGAGCTCCTGGA198790.24220925446726757No Hit
CACCCGTAGAACCGACCTTGCG197190.24025978614819907No Hit
ACCCCCCACTGCTAAATTTGACTG194420.23688476912081172No Hit
ACCGGGTGCTGTAGGCTT185960.226576955383737No Hit
CCCATAAAGTAGAAAGCACT184860.2252366959143774No Hit
AACATTCAACGCTGTCGGTGAGTT182130.22191041559496677No Hit
TCGTTTCCCGGCCAATGCACCA169230.20619282727247695No Hit
CCCATAAAGTAGAAAGCACTAC167610.20421899059942009No Hit
CACTAGATTGTGAGCTCCTGGAA167270.203804728581618No Hit
TGAGGTAGTAGTTTGTACAGTT162880.19845587488117383No Hit
TATTGCACTTGTCCCGGCCTGTAG162730.19827311222626112No Hit
AACGGAATCCCAAAAGCAGCTG162400.19787103438545328No Hit
TGAGAACTGAATTCCATGGGTT160940.19609214454430324No Hit
GGAATACCGGGTGCTGTAGGCTTA160930.19607996036730907No Hit
TATTGCACTTGTCCCGGCCTGTT156200.19031684464906282No Hit
CACCCGGCTGTGTGCACATGTGC153450.18696619597566383No Hit
TAGCTTATCAGACTGATGTTGACA152710.18606456687809464No Hit
AACCGGGCGGAAACACCA135160.16468133625331197No Hit
ACCGGGTGCTGTAGGCTTT134470.16384062804071367No Hit
ATCCCGGACGAGCCCCCA132250.16113574074800613No Hit
GGAATACCGGGTGCTGTAGGCT125830.15331349911774375No Hit
TGAGGTAGTAGTTTGTGCTGTT125310.15267992191404647No Hit
TAGCAGCACGTAAATATTGGC125120.1524484225511571No Hit
CCCCCCACTGCTAAATTTGACTGT122610.14939019412561838No Hit
ACCGGGTGCTGTAGGCTTA120690.14705083214273618No Hit
TATTGCACTTGTCCCGGCCTGTAAT118340.14418755054910432No Hit
CAAAGAATTCTCCTTTTGGGCT113970.13886306520264846No Hit
CCCCCACTGCTAAATTTGACTGGCTT113100.13780304180415495No Hit
CGCCCCACTGCTAAATTTGACTG109920.1339284735200063No Hit
TGATGTAGTAGATTGTATAGTT109530.13345329061723335No Hit
CGCCTGTCTGAGCGTCGCT107550.13104082357238606No Hit
ACTGGACTTGGAGTCAGAAGGCT106720.1300295368818693No Hit
TATTGCACTTGTCCCGGCCTGTAAA104870.12777546413794633No Hit
ACTGGACTTGGAGTCAGAAGGCA104350.12714188693424908No Hit
CTCCCCACTGCTAAATTTGACTG102960.1254482863320583No Hit
CCCGCCACTGCTAAATTTGACTG98820.12040403705646854No Hit
CGCGACCTCAGATCAGACGTGG98300.11977045985277128No Hit
TGTAAACATCCTACACTCTCAGCT97920.1193074611269925No Hit
CCCCCCACTGCTAAATTTGACTGG97550.11885664657820792No Hit
AGCTCGGTCTGAGGCCCCTCAGT97240.11847893709138839No Hit
TTCAAGTAATCCAGGATAGGCTT94370.11498207829405925No Hit
TGAGGTAGTAGGTTGTGTGGTT88220.10748880944263971No Hit
AGCAGCATTGTACAGGGCTATGT86230.10506415822079825No Hit
TATTGCACTTGTCCCGGCCTGTTT85870.10462552784900783No Hit
TGAGGTAGTAGGTTGTATAGT85870.10462552784900783No Hit
ACTTGACTTGGAGTCAGAAGGC82850.10094590639676602No Hit
ACCACAGGGTAGAACCACGGAC82610.10065348614890575No Hit
TTATAAAGCAATGAGACTGATT82420.10042198678601637No Hit
CTCCCACTGCTTCACTTGACTAG82310.1002879608390804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)