FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11097188
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG10422629.392127086609689No Hit
CCCCCCACTGCTAAATTTGACTG8319197.496664920879055No Hit
AAGCTGCCAGTTGAAGAACTGT4934104.44626152138722No Hit
TAGCAGCACGTAAATATTGGCG3963203.571355193766205No Hit
AACATTCAACGCTGTCGGTGAGT3739733.3699798543558965No Hit
TTCAAGTAATCCAGGATAGGCT3458903.116915744781471No Hit
CCCCCACTGCTAAATTTGACTG2604102.346630515766697No Hit
AACATTCAACGCTGTCGGTGAG2175521.9604245688186954No Hit
TAGCTTATCAGACTGATGTTGAC1812791.633557978832115No Hit
TAGCTTATCAGACTGATGTTGACT1404671.2657891350493478No Hit
TAGCTTATCAGACTGATGTTGA1317371.1871205570276No Hit
TATTGCACTTGTCCCGGCCTGTAA1265071.1399915005495085No Hit
GCCTGTCTGAGCGTCGCT1211001.0912674454104951No Hit
TATTGCACTTGTCCCGGCCTGTAT1195861.0776243495198965No Hit
TGAGGTAGTAGGTTGTATAGTT1023520.9223237454389346No Hit
TATTGCACTTGTCCCGGCCTGT1016900.9163582702212488No Hit
TGTAAACATCCTTGACTGGAAGCT898400.8095744615662994No Hit
AGCAGCATTGTACAGGGCTATGA852970.7686361626026341No Hit
CATAAAGTAGAAAGCACTACT699110.6299884258967227No Hit
CAACGGAATCCCAAAAGCAGCTGT642930.5793629881732201No Hit
TATTGCACTTGTCCCGGCCTGTA620410.5590695588828449No Hit
TGTAAACATCCCCGACTGGAAGCT591300.5328376882503928No Hit
CCCATAAAGTAGAAAGCACTAC533770.4809957261244921No Hit
TGAGGTAGTAGATTGTATAGTT531930.47933764842048276No Hit
TTCAAGTAATTCAGGATAGGTT524550.47268731502070616No Hit
TCCTGTCTGAGCGTCGCT424710.3827185769944602No Hit
GGAATACCGGGTGCTGTAGGCTTT392870.3540266236816029No Hit
TGATGTAGTAGGTTGTATAGTT389280.35079156990041077No Hit
ACTGGACTTGGAGTCAGAAGGC379830.3422758990836237No Hit
TGTAAACATCCTACACTCTCAGCT376510.3392841501829112No Hit
CGCCTGTCTGAGCGTCGCT350000.31539521543655924No Hit
CATTGCACTTGTCTCGGTCTGA325140.2929931438486939No Hit
AACATTCAACGCTGTCGGTGAGTT317830.28640588949200463No Hit
CCCCCACTGCTAAATTTGACTGGCTT291960.2630936774253081No Hit
CAACGGAATCCCAAAAGCAGCT291500.2626791579993058No Hit
GGAATACCGGGTGCTGTAGGCTT288920.26035424469694485No Hit
CCCATAAAGTAGAAAGCACT276730.249369479907883No Hit
TTCTCACTACTGCACTTGACTAG255830.23053587990038557No Hit
TAGCTTATCAGACTGATGTTGACA247880.22337190286404085No Hit
AGCTCGGTCTGAGGCCCCTCAGT243680.21958716027880215No Hit
TTCACAGTGGCTAAGTTCCG240690.21689278400978698No Hit
TGAGGTAGTAGTTTGTACAGTT238140.2145949045830349No Hit
TAGCAGCACGTAAATATTGGC235060.2118194266871932No Hit
CAACACCAGTCGATGGGCTGT226330.20395256888501842No Hit
CAAAGAATTCTCCTTTTGGGCT225150.20288923644440374No Hit
CTTCTCACTACTGCACTTGACTAG221570.19966319395508123No Hit
CTCCCACTGCTTCACTTGACTAG214850.1936076058186993No Hit
ACCCCCCACTGCTAAATTTGACTG206660.1862273577774838No Hit
TCGTTTCCCGGCCAATGCACCA204560.18433498648486446No Hit
TGATGTAGTAGATTGTATAGTT204540.18431696390112523No Hit
TATTGCACTTGTCCCGGCCTGTT203610.18347891375725095No Hit
CCCCCCACTGCTAAATTTGACTGGCT202620.18258679586215895No Hit
TGTAAACATCCCCGACTGGAAGC190140.1713407036088782No Hit
CTACGCCTGTCTGAGCGTCGCT185110.16680802379846138No Hit
AACCGGGCGGAAACACCA184520.16627635757815404No Hit
CCCCCCACTGCTAAATTTGACTGG180320.1624916149929153No Hit
TTCAAGTAATCCAGGATAGGCTT179700.16193291489699912No Hit
AACGGAATCCCAAAAGCAGCTG174820.15753540446462655No Hit
TGTAAACATCCTACACTCAGCT172490.15543577345900603No Hit
TTCAAGTAATCCAGGATAGGC168910.1522097309696835No Hit
TAGCAGCACGTAAATATTGGCGT165410.1490557788153179No Hit
TGAGGTAGTAGTTTGTGCTGTT164410.1481546496283563No Hit
AAGCTGCCAGTTGAAGCACTGT163790.1475959495324401No Hit
ACTTGACTTGGAGTCAGAAGGC161390.14543323948373227No Hit
CAAAGAATTCTCCTTTTGGGCTT155650.1402607579505727No Hit
AGCAGCATTGTACAGGGCTATGT153780.1385756463709545No Hit
TGAATACCGGGTGCTGTAGGCTTT153670.13847652216038875No Hit
AGCAGCATTGTACAGGGCTATG141900.12787023162985073No Hit
CACTAGATTGTGAGCTCCTGGA141690.1276809945005888No Hit
TGCAAGTAATCCAGGATAGGCT137760.1241395567958297No Hit
GTCAGGATGGCCGAGCGGTCTAAGG137160.12359887928365276No Hit
TATTGCACTTGTCCCGGCCTGTAG133660.12044492712928717No Hit
ACCACAGGGTAGAACCACGGAC132090.11903015430575746No Hit
TGTCAGTTTGTCAAATACCCCA131130.11816507028627433No Hit
TACGCCTGTCTGAGCGTCGCT130930.11798484444888202No Hit
GGGCTACGCCTGTCTGAGCGTCGCT130170.11729998626679118No Hit
CACTAGATTGTGAGCTCCTGGAA129290.11650699258226498No Hit
ATCACATTGCCAGGGATTTCC123030.11086592387188539No Hit
ATCACATTGCCAGGGATTTCCA122930.11077581095318921No Hit
CAAAGTGCTGTTCGTGCAGGTAG119510.10769394913378055No Hit
TATTGCACTTGTCCCGGCCTGTTT119230.10744163296143132No Hit
CAAAGTGCTGTTCGTGCAGGT118780.10703612482729859No Hit
TCGTACGACTCTTAGCGG118680.10694601190860244No Hit
TTCACAGTGGCTAAGTTCTGC118400.10669369573625317No Hit
AACATTCAACGCTGTCGGTGAGA116160.1046751663574592No Hit
ACGCCTGTCTGAGCGTCGCT115430.10401734205097724No Hit
ACTGGACTTGGAGTCAGAAGGCA113210.1020168352559225No Hit
TGAATACCGGGTGCTGTAGGCTT113180.10198980138031363No Hit
TTATAAAGCAATGAGACTGATT113120.10193573362909596No Hit
AACATTCATTGCTGTCGGTGGGTT111510.10048491563808779No Hit
TGTCAGTTTGTCAAATACCCCAA111260.10025963334134738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)