FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10478858
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG242630723.154307463656824No Hit
CAACGGAATCCCAAAAGCAGCTG7259526.927777817010212No Hit
CCCCCACTGCTAAATTTGACTG6136355.8559339195168025No Hit
TAGCAGCACGTAAATATTGGCG2686052.56330413104176No Hit
TTCAAGTAATCCAGGATAGGCT2395192.2857357166210286No Hit
AAGCTGCCAGTTGAAGAACTGT2159072.0604058190310433No Hit
AACATTCAACGCTGTCGGTGAGT1993561.9024592183613902No Hit
TATTGCACTTGTCCCGGCCTGTAT1734501.6552376222676173No Hit
TAGCTTATCAGACTGATGTTGACT1686351.6092879586687787No Hit
AACATTCAACGCTGTCGGTGAG1613541.5398051963296No Hit
TATTGCACTTGTCCCGGCCTGTAA1485331.4174540775340214No Hit
TAGCTTATCAGACTGATGTTGAC1354081.2922018792505825No Hit
TAGCTTATCAGACTGATGTTGA1286901.228091839778724No Hit
GGAATACCGGGTGCTGTAGGCTTT1274091.2158672252262603No Hit
TGAGGTAGTAGGTTGTATAGTT1000030.9543310921858087No Hit
GGAATACCGGGTGCTGTAGGCTT932230.8896293851868209No Hit
TATTGCACTTGTCCCGGCCTGT926990.8846288402801146No Hit
TATTGCACTTGTCCCGGCCTGTA801420.7647970799871512No Hit
TGAGGTAGTAGATTGTATAGTT738460.7047141969096251No Hit
TGTAAACATCCTTGACTGGAAGCT736300.7026529035893033No Hit
TTCAAGTAATTCAGGATAGGTT587440.5605954389304636No Hit
GCCTGTCTGAGCGTCGCT559450.533884512987961No Hit
AGCAGCATTGTACAGGGCTATGA542150.517375080376125No Hit
TTCTCACTACTGCACTTGACTAG537310.5127562564546633No Hit
TGTAAACATCCCCGACTGGAAGCT509270.4859976153890052No Hit
CATAAAGTAGAAAGCACTACT496440.4737539147872793No Hit
TGAATACCGGGTGCTGTAGGCTTT485450.4632661307176793No Hit
CTTCTCACTACTGCACTTGACTAG403460.3850228717671334No Hit
GACCCGCGGGCGCTCTCCAGTCCT401100.38277071795418927No Hit
CAACGGAATCCCAAAAGCAGCTGT368890.3520326356173545No Hit
TGAATACCGGGTGCTGTAGGCTT361820.34528571720315326No Hit
CCCCCCACTGCTAAATTTGACTGG345260.32948246841401996No Hit
ACTGGACTTGGAGTCAGAAGGC340980.3253980538719009No Hit
CTCCCACTGCTTCACTTGACTAG339160.32366122338903724No Hit
ACCCCCCACTGCTAAATTTGACTG312500.29821951972247357No Hit
CATTGCACTTGTCTCGGTCTGA294410.280956188164779No Hit
TGATGTAGTAGGTTGTATAGTT266120.25395897148334295No Hit
CCCATAAAGTAGAAAGCACTAC264630.2525370608133062No Hit
TGAGGTAGTAGTTTGTACAGTT251770.24026473113768695No Hit
GGAATACCGGGTGCTGTAGGCTTA240760.22975786101882478No Hit
CCCCCCACTGCTAAATTTGACTGT228630.21818217214127725No Hit
CACTAGATTGTGAGCTCCTGGA226630.2162735672150534No Hit
CAACGGAATCCCAAAAGCAGCT222110.21196012008178755No Hit
TATTGCACTTGTCCCGGCCTGTAG221720.21158794212117388No Hit
CAAAGAATTCTCCTTTTGGGCT217890.20793296368745526No Hit
CCCCCACTGCTAAATTTGACTGGCTT216120.20624384832774717No Hit
CACTAGATTGTGAGCTCCTGGAA215220.20538497611094644No Hit
AGCTCGGTCTGAGGCCCCTCAGT206900.19744517961785532No Hit
TATTGCACTTGTCCCGGCCTGTT204090.1947635896965108No Hit
CCCATAAAGTAGAAAGCACT201980.19275001149934468No Hit
CCCCCCACTGCTAAATTTGACTGA198970.18987756108537782No Hit
TGATGTAGTAGATTGTATAGTT198280.1892190923858306No Hit
TGTAAACATCCTACACTCTCAGCT196450.1874727188783358No Hit
CAACACCAGTCGATGGGCTGT196100.18713871301624663No Hit
CCCCCCACTGCTAAATTTGACTGGCT190120.18143198428683735No Hit
TAGCTTATCAGACTGATGTTGACA183560.17517176012882318No Hit
TAGCAGCACGTAAATATTGGC180450.17220387946854515No Hit
TCCTGTCTGAGCGTCGCT179130.1709442002172374No Hit
TATTGCACTTGTCCCGGCCTGTTT172310.16443585741881414No Hit
GGAATACCGGGTGCTGTAGGCT167390.15974068930030352No Hit
TGTAAACATCCCCGACTGGAAGC163780.1562956574084695No Hit
CCCCCCACTGCTAAATTTGACTGTA163520.1560475387680604No Hit
TCGTACGACTCTTAGCGG162930.1554845003148244No Hit
AACGGAATCCCAAAAGCAGCTG157610.150407611211069No Hit
CAAAGAATTCTCCTTTTGGGCTT157100.14992091695488194No Hit
GCCCCCACTGCTAAATTTGACTG148520.14173300182138168No Hit
TGAGGTAGTAGTTTGTGCTGTT146180.1394999340576998No Hit
TTCAAGTAATCCAGGATAGGCTT134750.1285922569043306No Hit
AACCGGGCGGAAACACCA132500.1264450763623288No Hit
AACATTCAACGCTGTCGGTGAGTT130100.12415475045086019No Hit
CCCCCCACTGCTAAATTTGAC128860.12297141539660142No Hit
TATTGCACTTGTCCCGGCCTGTAAT125460.11972678702202091No Hit
CCCCCACAACCGCGCTTGACTAG125040.11932597998751392No Hit
TGTCAGTTTGTCAAATACCCCAA119980.1144972095241676No Hit
AGCAGCATTGTACAGGGCTATGT118900.11346656286400675No Hit
TGTCAGTTTGTCAAATACCCCA115010.1097543262825014No Hit
TTATAAAGCAATGAGACTGATT112160.10703456426263244No Hit
CACCCGGCTGTGTGCACATGTGC111610.10650969790792088No Hit
ACTGGACTTGGAGTCAGAAGGCT109100.10411439872550997No Hit
CTCCCACTGCTTCACTTGACTAGCCT107020.10212944960223719No Hit
TTCAAGTAATCCAGGATAGGC106760.10188133096182811No Hit
CCCGCCACTGCTAAATTTGACTG105830.10099382967113402No Hit
ACTGGACTTGGAGTCAGAAGGCA105480.10065982380904484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)