FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6219259
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG80336912.917439199750325No Hit
CAACGGAATCCCAAAAGCAGCTG3853206.195593397862993No Hit
CCCCCACTGCTAAATTTGACTG2580824.1497226598860095No Hit
AAGCTGCCAGTTGAAGAACTGT2142503.444944164570088No Hit
AACATTCAACGCTGTCGGTGAGT1813122.915331231582412No Hit
TAGCAGCACGTAAATATTGGCG1758112.8268801797770444No Hit
TTCAAGTAATCCAGGATAGGCT1674532.6924911794154256No Hit
TAGCTTATCAGACTGATGTTGAC1004041.6144045456219143No Hit
AACATTCAACGCTGTCGGTGAG905931.45665263337642No Hit
TAGCTTATCAGACTGATGTTGACT888791.4290930800598594No Hit
TAGCTTATCAGACTGATGTTGA850721.3678799998520725No Hit
TATTGCACTTGTCCCGGCCTGTAA584120.9392115684521258No Hit
TGAGGTAGTAGGTTGTATAGTT565740.909658208477891No Hit
CGCCTGTCTGAGCGTCGCT563850.9066192612335328No Hit
TATTGCACTTGTCCCGGCCTGT563700.906378074944298No Hit
TATTGCACTTGTCCCGGCCTGTAT531100.8539602547506062No Hit
TGTAAACATCCTTGACTGGAAGCT432520.6954526254655097No Hit
AGCAGCATTGTACAGGGCTATGA395250.6355258721336416No Hit
CATAAAGTAGAAAGCACTACT380770.612243355679511No Hit
TATTGCACTTGTCCCGGCCTGTA338640.5445021665764361No Hit
TGAGGTAGTAGATTGTATAGTT313680.5043687680477691No Hit
ACTGGACTTGGAGTCAGAAGGC290110.46647036246601087No Hit
TTCAAGTAATTCAGGATAGGTT288820.4643961603785917No Hit
TGTAAACATCCCCGACTGGAAGCT249230.40073905910655916No Hit
TTCTCACTACTGCACTTGACTAG240580.38683064976068693No Hit
TCGTACGACTCTTAGCGG235990.3794503493101027No Hit
CAACGGAATCCCAAAAGCAGCTGT224790.36144177304723923No Hit
CTTCTCACTACTGCACTTGACTAG187480.3014507033715753No Hit
CATTGCACTTGTCTCGGTCTGA181330.2915620655129494No Hit
CCCATAAAGTAGAAAGCACTAC179250.2882176156355604No Hit
CAACACCAGTCGATGGGCTGT176450.28371547156984456No Hit
TACGCCTGTCTGAGCGTCGCT159410.2563167091127737No Hit
TTAATGCTAATCGTGATAGGGGTT151120.24298714686106496No Hit
CCCCCCACTGCTAAATTTGACTGG148740.23916032440520646No Hit
CTCCCACTGCTTCACTTGACTAG147910.2378257602714407No Hit
TGTAAACATCCTACACTCTCAGCT147400.23700572688804245No Hit
GACCCGCGGGCGCTCTCCAGTCCT145480.2339185423858373No Hit
CCCATAAAGTAGAAAGCACT142140.22854812767887622No Hit
CTACGCCTGTCTGAGCGTCGCT141400.22735827531865127No Hit
ACGCCTGTCTGAGCGTCGCT137830.22161804163486357No Hit
AACATTCAACGCTGTCGGTGAGTT136330.2192061787425158No Hit
ACCGGGTGCTGTAGGCTT135950.21859517347645438No Hit
CAACGGAATCCCAAAAGCAGCT135650.21811280089798477No Hit
GGAATACCGGGTGCTGTAGGCTTT135130.21727668842863754No Hit
TGAGGTAGTAGTTTGTACAGTT125670.20206587312089752No Hit
TGATGTAGTAGGTTGTATAGTT116850.18788411931389254No Hit
CGCCCCACTGCTAAATTTGACTG116030.18656563426607575No Hit
TAGCTTATCAGACTGATGTTGACA115610.1858903126562184No Hit
GGAATACCGGGTGCTGTAGGCTT112200.1804073443476144No Hit
TTCAAGTAATCCAGGATAGGCTT107320.1725607504045096No Hit
GCGTTCGCGCTTTCCCCTG106190.1707438136922743No Hit
TAGCAGCACGTAAATATTGGC97030.1560153709630038No Hit
CCCCCACTGCTAAATTTGACTGGCTT96200.15468080682923802No Hit
ACCCCCCACTGCTAAATTTGACTG95790.15402156430532962No Hit
TGAGGTAGTAGTTTGTGCTGTT95010.15276739560130878No Hit
AGCTCGGTCTGAGGCCCCTCAGT94270.15157754324108388No Hit
AACGGAATCCCAAAAGCAGCTG93490.15032337453706301No Hit
ACCGGGTGCTGTAGGCTTT92480.14869938685621553No Hit
TATTGCACTTGTCCCGGCCTGTT88420.1421712779609275No Hit
CTCCCCACTGCTAAATTTGACTG86440.1389876189430284No Hit
CCCCCCACTGCTAAATTTGACTGGCT85780.13792639927039538No Hit
TTATAAAGCAATGAGACTGATT85600.13763697572331365No Hit
ACCACAGGGTAGAACCACGGAC84450.13578788083918036No Hit
GCCTGTCTGAGCGTCGCT84190.13536982460450675No Hit
AGCAGCATTGTACAGGGCTATGT83730.1346301866508534No Hit
ACCGGGTGCTGTAGGCTTA81890.13167163483624014No Hit
TGAATACCGGGTGCTGTAGGCTTT81410.13089983871068883No Hit
CAAAGAATTCTCCTTTTGGGCT80600.12959743274882105No Hit
CAAAGAATTCTCCTTTTGGGCTT76140.12242616041557362No Hit
AGCAGCATTGTACAGGGCTATG72080.11589805152028561No Hit
TTCAAGTAATCCAGGATAGGC71280.11461172464436678No Hit
CCCGCCACTGCTAAATTTGACTG71110.11433838018323406No Hit
CAAAGTGCTGTTCGTGCAGGT70640.11358266314363175No Hit
TATTGCACTTGTCCCGGCCTGTAG70360.11313244873706015No Hit
ATCCCGGACGAGCCCCCA70190.1128591042759274No Hit
CCCCCCACTGCTAAATTTGACTGTA69160.11120295842318192No Hit
TGTAAACATCCCCGACTGGAAGC68000.10933778445309963No Hit
TGAATACCGGGTGCTGTAGGCTT67980.10930562628120168No Hit
TGATGTAGTAGATTGTATAGTT67650.10877501644488516No Hit
CCCTCCACTGCTAAATTTGACTG66870.1075208477408643No Hit
TTCACAGTGGCTAAGTTCCG66430.10681336795910895No Hit
ACTTGACTTGGAGTCAGAAGGC65440.10522153845015941No Hit
TAGCAGCACGTAAATATTGGCGT65230.10488387764523073No Hit
TGAGGTAGTAGGTTGTGTGGTT64020.10293830824540352No Hit
CCCCCCACTGCTAAATTTTACTG63260.10171629771328064No Hit
TGCAAGTAATCCAGGATAGGCT62740.10088018524393341No Hit
TTCACAGTGGCTAAGTTCTGC62370.10028525906382094No Hit
GCCCCCACTGCTAAATTTGACTG62360.10026917997787196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)