FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8568665
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG173829120.286602405392205No Hit
CAACGGAATCCCAAAAGCAGCTG8333229.725225574812413No Hit
CCCCCACTGCTAAATTTGACTG5015355.853128812948108No Hit
AACATTCAACGCTGTCGGTGAGT2432682.8390420211316463No Hit
AAGCTGCCAGTTGAAGAACTGT2347592.739738337302252No Hit
TAGCAGCACGTAAATATTGGCG2257212.634260996316229No Hit
TTCAAGTAATCCAGGATAGGCT2101722.4527974894572258No Hit
AACATTCAACGCTGTCGGTGAG1646281.9212794525168158No Hit
TATTGCACTTGTCCCGGCCTGTAA1046091.2208319499011806No Hit
TATTGCACTTGTCCCGGCCTGTAT950941.1097878140877255No Hit
TGAGGTAGTAGGTTGTATAGTT789610.9215087764546752No Hit
TAGCTTATCAGACTGATGTTGACT763900.8915041024476975No Hit
TAGCTTATCAGACTGATGTTGAC734580.8572864034245709No Hit
TATTGCACTTGTCCCGGCCTGT707370.8255311650064509No Hit
TAGCTTATCAGACTGATGTTGA695130.8112465594115303No Hit
TATTGCACTTGTCCCGGCCTGTA453800.5296040865175613No Hit
TGAGGTAGTAGATTGTATAGTT438520.5117716703827259No Hit
CAACGGAATCCCAAAAGCAGCTGT413350.48239719956375937No Hit
TGTAAACATCCTTGACTGGAAGCT386680.4512721643336505No Hit
AGCAGCATTGTACAGGGCTATGA352010.41081078557745No Hit
GACCCGCGGGCGCTCTCCAGTCCT350920.4095387087720199No Hit
TTCTCACTACTGCACTTGACTAG350730.4093169706132752No Hit
TTCAAGTAATTCAGGATAGGTT327050.38168139377604327No Hit
TGTAAACATCCCCGACTGGAAGCT323090.3770599037306278No Hit
ACTGGACTTGGAGTCAGAAGGC307090.3583872166784441No Hit
CTTCTCACTACTGCACTTGACTAG295070.34435936053049104No Hit
CATAAAGTAGAAAGCACTACT273560.31925626687471154No Hit
CAACGGAATCCCAAAAGCAGCT270240.31538168431138336No Hit
CCCCCCACTGCTAAATTTGACTGG260600.3041313903624427No Hit
TTAATGCTAATCGTGATAGGGGTT253710.29609046450059606No Hit
ACCCCCCACTGCTAAATTTGACTG242840.2834047077345187No Hit
CATTGCACTTGTCTCGGTCTGA238180.2779662876305702No Hit
GGAATACCGGGTGCTGTAGGCTTT236680.276215723219428No Hit
CCCATAAAGTAGAAAGCACTAC222660.259853781189952No Hit
CTCCCACTGCTTCACTTGACTAG218250.25470712182119387No Hit
AACGGAATCCCAAAAGCAGCTG193550.2258811611843852No Hit
GGAATACCGGGTGCTGTAGGCTT186440.21758348587557103No Hit
CCCCCACTGCTAAATTTGACTGGCTT183980.2147125602412978No Hit
TCGTACGACTCTTAGCGG182590.21309037055363933No Hit
CCCATAAAGTAGAAAGCACT170720.19923757084680055No Hit
GCCCCCACTGCTAAATTTGACTG169500.1978137784590715No Hit
TGAGGTAGTAGTTTGTACAGTT169490.19780210802966391No Hit
CCCGCCACTGCTAAATTTGACTG168150.1962382704890435No Hit
CGCCCCACTGCTAAATTTGACTG161290.18823235591541973No Hit
TGAGAACTGAATTCCATGGGTT155180.18110172354736706No Hit
CAACACCAGTCGATGGGCTGT152700.17820745705427857No Hit
TAGCAGCACGTAAATATTGGC145920.17029490591591573No Hit
AACATTCAACGCTGTCGGTGAGTT136980.15986154202550806No Hit
TGAGGTAGTAGTTTGTGCTGTT136040.15876452166119226No Hit
CCCCCCACTGCTAAATTTTACTG134570.15704896853827288No Hit
CACTAGATTGTGAGCTCCTGGA132160.15423639505103773No Hit
CCCCCCACTGCTAAATTTGACTGT131200.15311603382790667No Hit
CCCCCCACTGCTAAATTTGACTGTA130800.1526492166516021No Hit
CCCCCCACTGCTAAATTTGACTGGCT130580.15239246720463456No Hit
TATTGCACTTGTCCCGGCCTGTAG130500.15229910376937364No Hit
CAAAGAATTCTCCTTTTGGGCT130210.1519606613165528No Hit
AACCGGGCGGAAACACCA118140.1378744530215617No Hit
TATTGCACTTGTCCCGGCCTGTT117790.13746598799229517No Hit
TGTAAACATCCTACACTCTCAGCT117720.1373842949864419No Hit
AGCTCGGTCTGAGGCCCCTCAGT115810.13515524296958745No Hit
TGATGTAGTAGGTTGTATAGTT113960.1329962135291787No Hit
CACTAGATTGTGAGCTCCTGGAA109200.12744108913115404No Hit
TCGTTTCCCGGCCAATGCACCA107740.12573720643764227No Hit
CACCCGGCTGTGTGCACATGTGC107700.1256905247200118No Hit
TACCCGCGGGCGCTCTCCAGTCCT106790.12462851564391886No Hit
TGTAAACATCCCCGACTGGAAGC105710.12336810926789644No Hit
TTCAAGTAATCCAGGATAGGCTT100620.1174278606994205No Hit
CAAAGAATTCTCCTTTTGGGCTT99590.11622580647043616No Hit
TGAATACCGGGTGCTGTAGGCTTT99500.11612077260576764No Hit
ACTGGACTTGGAGTCAGAAGGCT99310.11589903444702296No Hit
ACCGGGTGCTGTAGGCTT98070.11445190120047873No Hit
TAGCTTATCAGACTGATGTTGACA97370.11363497114194568No Hit
CCCACCACTGCTAAATTTGACTG95640.11161598685442832No Hit
GGGAATACCGGGTGCTGTAGGCTTT93020.10855833434963323No Hit
AACATTCAACGCTGTCGGTGA92990.10852332306141038No Hit
ACCGGGTGCTGTAGGCTTA89710.10469542221571272No Hit
TTCAAGTAATCCAGGATAGGC89690.10467208135689748No Hit
CCACCCACTGCTAAATTTGACTG88330.10308490295746187No Hit
CCCCCCACTGCTAAATTTGACTGA88000.10269977878701057No Hit
CAACGGAATCCCAAAAGCAGCTGA86880.10139269069335771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)