FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8230569
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG152400818.516435497958888No Hit
CAACGGAATCCCAAAAGCAGCTG6404597.781466870637983No Hit
CCCCCACTGCTAAATTTGACTG5808587.057324955297744No Hit
AACATTCAACGCTGTCGGTGAGT2479953.0130966643982937No Hit
AACATTCAACGCTGTCGGTGAG1867222.2686402361732223No Hit
TTCAAGTAATCCAGGATAGGCT1802132.1895570038960854No Hit
TAGCAGCACGTAAATATTGGCG1782282.1654395947594876No Hit
AAGCTGCCAGTTGAAGAACTGT1770542.1511756963582958No Hit
TAGCTTATCAGACTGATGTTGAC1335361.6224394692517612No Hit
TAGCTTATCAGACTGATGTTGACT1250031.518764984535091No Hit
TAGCTTATCAGACTGATGTTGA1219431.4815865099970609No Hit
GCCTGTCTGAGCGTCGCT1188301.4437640945601695No Hit
TGAGGTAGTAGGTTGTATAGTT1001851.2172305462720743No Hit
TATTGCACTTGTCCCGGCCTGTAA935831.1370173799648604No Hit
TATTGCACTTGTCCCGGCCTGTAT823291.0002832124972163No Hit
GGAATACCGGGTGCTGTAGGCTTT772420.9384770360348111No Hit
TATTGCACTTGTCCCGGCCTGT713450.8668294986652806No Hit
GGAATACCGGGTGCTGTAGGCTT615120.7473602371840877No Hit
TGAGGTAGTAGATTGTATAGTT556490.6761257939760908No Hit
TATTGCACTTGTCCCGGCCTGTA542640.6592982818077341No Hit
TCCTGTCTGAGCGTCGCT483710.587699343751325No Hit
AGCAGCATTGTACAGGGCTATGA419940.5102198888072016No Hit
CATAAAGTAGAAAGCACTACT411720.5002327299607111No Hit
TGTAAACATCCTTGACTGGAAGCT397540.4830042734591011No Hit
CATTGCACTTGTCTCGGTCTGA371700.45160911718254226No Hit
ACTGGACTTGGAGTCAGAAGGC342220.4157914234119172No Hit
CAACGGAATCCCAAAAGCAGCT331300.4025238109297182No Hit
TGAATACCGGGTGCTGTAGGCTTT326310.3964610466177976No Hit
CAACACCAGTCGATGGGCTGT320670.3896085434676509No Hit
TTCAAGTAATTCAGGATAGGTT320030.38883095445770516No Hit
TGTAAACATCCCCGACTGGAAGCT315770.38365512761025394No Hit
CAACGGAATCCCAAAAGCAGCTGT275620.3348735670644399No Hit
TCGTACGACTCTTAGCGG259190.31491139919973943No Hit
TGAATACCGGGTGCTGTAGGCTT253500.30799814690819066No Hit
TTAATGCTAATCGTGATAGGGGTT247280.3004409537177806No Hit
TTCTCACTACTGCACTTGACTAG232750.2827872532263565No Hit
CCCATAAAGTAGAAAGCACT224170.2723627005617716No Hit
TGATGTAGTAGGTTGTATAGTT198940.24170868381031738No Hit
GACCCGCGGGCGCTCTCCAGTCCT185320.22516061769240983No Hit
TGAGGTAGTAGTTTGTACAGTT183950.22349609121799477No Hit
CTTCTCACTACTGCACTTGACTAG183290.22269420255148822No Hit
CCCATAAAGTAGAAAGCACTAC179980.21867260939067518No Hit
AACGGAATCCCAAAAGCAGCTG174370.21185655572536966No Hit
CGCCCCACTGCTAAATTTGACTG171130.20792001136251942No Hit
ACCCCCCACTGCTAAATTTGACTG169280.20567229313064503No Hit
TGAGGTAGTAGTTTGTGCTGTT158670.1927813253251385No Hit
CACTAGATTGTGAGCTCCTGGA158040.19201588614347317No Hit
TAGCTTATCAGACTGATGTTGACA155260.18863823388152146No Hit
CCCCCCACTGCTAAATTTGACTGG153280.18623256788200185No Hit
AACATTCAACGCTGTCGGTGAGTT150210.18250257059991842No Hit
CTCCCCACTGCTAAATTTGACTG144730.17584446470225815No Hit
TGTCAGTTTGTCAAATACCCCAA138130.1678255780371928No Hit
TGAGAACTGAATTCCATGGGTT136010.16524981444174758No Hit
CAAAGAATTCTCCTTTTGGGCT131230.1594421965237154No Hit
TAGCAGCACGTAAATATTGGC128790.15647763842329734No Hit
GGAATACCGGGTGCTGTAGGCTTA128250.15582154769615564No Hit
CCCGCCACTGCTAAATTTGACTG120880.14686712425349935No Hit
TATTGCACTTGTCCCGGCCTGTT119630.14534839571844912No Hit
CTCCCACTGCTTCACTTGACTAG118320.14375676821371644No Hit
CACTAGATTGTGAGCTCCTGGAA118290.14372031872887525No Hit
TGTAAACATCCTACACTCTCAGCT117820.14314927679969636No Hit
ACCGGGTGCTGTAGGCTT117220.14242028710287222No Hit
GCCCCCACTGCTAAATTTGACTG117140.142323088476629No Hit
TATTGCACTTGTCCCGGCCTGTAG116420.14144830084044008No Hit
CCCCCCACTGCTAAATTTGACTGT112780.13702576334637373No Hit
TGATGTAGTAGATTGTATAGTT110670.13446214957920893No Hit
CCCCCCACTGCTAAATTTGACTGTA110250.13395185679143204No Hit
ACTGGACTTGGAGTCAGAAGGCT107350.13042840659011545No Hit
CCCCCACTGCTAAATTTGACTGGCTT106950.12994241345889937No Hit
GGAATACCGGGTGCTGTAGGCT103470.12571427321731948No Hit
TTATAAAGCAATGAGACTGATT102360.12436564227819487No Hit
TTCAAGTAATCCAGGATAGGCTT101480.12329645738951948No Hit
TTCACAGTGGCTAAGTTCTG96150.11682059891606521No Hit
CAAAGAATTCTCCTTTTGGGCTT95940.11656545252217677No Hit
CACCCGGCTGTGTGCACATGTGC95430.11594581127987627No Hit
TGTCAGTTTGTCAAATACCCCA90720.11022324215980693No Hit
AACATTCAACGCTGTCGGTGA90650.11013819336184412No Hit
ACCGGGTGCTGTAGGCTTT87370.10615304968587226No Hit
ACTGGACTTGGAGTCAGAAGGCA87180.10592220294854462No Hit
TGAGGTAGTAGGTTGTATAGT86370.10493806685783207No Hit
AACCGGGCGGAAACACCA85550.10394178093883912No Hit
AGCAGCATTGTACAGGGCTATG84570.10275109776735969No Hit
TTCACAGTGGCTAAGTTCTGC83720.10171836236352552No Hit
ACCGGGTGCTGTAGGCTTA83120.10098937266670141No Hit
AATGCACCCGGGCAAGGATTCT83090.10095292318186022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)