FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6119506
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG68157111.137680067639447No Hit
GCCTGTCTGAGCGTCGCT3792186.196872754107929No Hit
TTCAAGTAATCCAGGATAGGCT2803124.580631181667278No Hit
TAGCAGCACGTAAATATTGGCG1950523.18738146510519No Hit
CCCCCCACTGCTAAATTTGACTG1936223.164013565800899No Hit
AAGCTGCCAGTTGAAGAACTGT1860353.040032969981564No Hit
AACATTCAACGCTGTCGGTGAGT1498592.4488741411479946No Hit
AACATTCAACGCTGTCGGTGAG1242882.031013614497641No Hit
TATTGCACTTGTCCCGGCCTGTAA1110191.8141823866174818No Hit
TGAGGTAGTAGGTTGTATAGTT1104521.804916932837389No Hit
TATTGCACTTGTCCCGGCCTGTAT998161.6311120538161088No Hit
TAGCTTATCAGACTGATGTTGACT786631.2854468971841844No Hit
TAGCTTATCAGACTGATGTTGAC785051.2828649894288853No Hit
AACCGGGCGGAAACACCA753381.2311124460046285No Hit
TGAGGTAGTAGATTGTATAGTT677021.1063311319573836No Hit
CCCCCACTGCTAAATTTGACTG619151.0117646751224691No Hit
TAGCTTATCAGACTGATGTTGA581950.950975454554665No Hit
TGTAAACATCCTTGACTGGAAGCT566320.9254341772031925No Hit
TATTGCACTTGTCCCGGCCTGT553410.9043377030760327No Hit
TTCAAGTAATTCAGGATAGGTT475660.7772849638516574No Hit
CAACGGAATCCCAAAAGCAGCTGT448250.7324937666537136No Hit
CATAAAGTAGAAAGCACTACT431380.7049261819499809No Hit
TATTGCACTTGTCCCGGCCTGTA383130.6260799482834072No Hit
AGCAGCATTGTACAGGGCTATGA377570.616994247574886No Hit
TGTAAACATCCCCGACTGGAAGCT304590.4977362551813823No Hit
TCGTACGACTCTTAGCGG295160.48232651459121045No Hit
ACTGGACTTGGAGTCAGAAGGC292080.47729342858720947No Hit
CCCATAAAGTAGAAAGCACTAC286190.4676684686639738No Hit
CCCCCACTGCTAAATTTGACTGGCTT278320.45480795345245184No Hit
GGAATACCGGGTGCTGTAGGCTTT261430.42720768637206985No Hit
TGATGTAGTAGGTTGTATAGTT253930.414951795128561No Hit
TGAGGTAGTAGTTTGTACAGTT231470.37824948615133314No Hit
CTCCCACTGCTTCACTTGACTAG231040.37754681505337195No Hit
CATTGCACTTGTCTCGGTCTGA212640.3474790285359635No Hit
TGTAAACATCCTACACTCTCAGCT175190.28628127826004257No Hit
CGCCTGTCTGAGCGTCGCT170160.2780616605327293No Hit
GGAATACCGGGTGCTGTAGGCTT161860.2644984742232461No Hit
TTCTCACTACTGCACTTGACTAG158890.2596451412908166No Hit
TAGCAGCACGTAAATATTGGC158660.259269293959349No Hit
TGATGTAGTAGATTGTATAGTT153940.25155625307010077No Hit
AGCTCGGTCTGAGGCCCCTCAGT153160.2502816403807758No Hit
CACCCGGCTGTGTGCACATGTGC151690.2478794856970481No Hit
CTACGCCTGTCTGAGCGTCGCT151550.24765070906050257No Hit
CAACGGAATCCCAAAAGCAGCT149440.24420271832399543No Hit
AACGGAATCCCAAAAGCAGCTG148390.24248689354990421No Hit
CCCATAAAGTAGAAAGCACT147290.24068936283418957No Hit
CCCCCCACTGCTAAATTTGACTGGCT141470.23117879122922666No Hit
CAAAGAATTCTCCTTTTGGGCT141280.23086830865105779No Hit
TTCAAGTAATCCAGGATAGGC125710.20542507842953336No Hit
TGAGGTAGTAGTTTGTGCTGTT124170.20290853542753287No Hit
CTTCTCACTACTGCACTTGACTAG121310.19823495556667484No Hit
TTCAAGTAATCCAGGATAGGCTT120420.19678058980577845No Hit
TATTGCACTTGTCCCGGCCTGTAG115370.1885282897018158No Hit
TAGCTTATCAGACTGATGTTGACA114870.18771123028558187No Hit
CTCCCACTGCTTCACTTGACTAGCCT111350.18195913199529504No Hit
TTCACAGTGGCTAAGTTCTGC106900.17468730319081313No Hit
TCGTTTCCCGGCCAATGCACCA106660.17429511467102085No Hit
TATTGCACTTGTCCCGGCCTGTT104860.1713537007725787No Hit
AACATTCAACGCTGTCGGTGAGTT103760.16955617005686405No Hit
TGAGGTAGTAGGTTGTGTGGTT101360.16563428485894124No Hit
CACTAGATTGTGAGCTCCTGGA100140.16364065988333046No Hit
CACTAGATTGTGAGCTCCTGGAA98800.16145094064782353No Hit
TATTGCACTTGTCCCGGCCTGTAAT96150.15712052574178373No Hit
TGAGGTAGTAGGTTGTATAGT95680.15635248989052383No Hit
CAACACCAGTCGATGGGCTGT95600.15622176038392643No Hit
TTCACAGTGGCTAAGTTCCGC94030.1536561938169519No Hit
TCGTTTCCCGGGCGGCGCACCA90850.14845969592970412No Hit
TACGCCTGTCTGAGCGTCGCT88890.14525682301806714No Hit
CAAAGAATTCTCCTTTTGGGCTT86630.1415637144566898No Hit
ATCACATTGCCAGGGATTTCC86380.14115518474857283No Hit
TTATAAAGCAATGAGACTGATT86260.1409590904886767No Hit
CCCCCCACTGCTAAATTTGACTGG84840.13863864174657237No Hit
TATTGCACTTGTCCCGGCCTGTAAA83960.13720061717400064No Hit
TGTAAACATCCCCGACTGGAAGC83510.1364652636993901No Hit
AGCAGCATTGTACAGGGCTATGT81760.13360555574257138No Hit
TGAGGTAGTAAGTTGTATTGTT81730.13355653217759733No Hit
TATTGCACTTGTCCCGGCCTGTTT77710.12698737447107658No Hit
TGTCAGTTTGTCAAATACCCCAA77070.12594153841829717No Hit
ACCCCCCACTGCTAAATTTGACTG76320.12471594929394628No Hit
ATGCACCTGGGCAAGGATTCTGA72290.11813045039910085No Hit
TGTCAGTTTGTCAAATACCCCA71510.11685583770977591No Hit
CAAAGTGCTGTTCGTGCAGGTAG69580.11370198836311296No Hit
ACTTGACTTGGAGTCAGAAGGC68060.11121812773776184No Hit
AGAGGTAGTAGGTTGCATAGTT67840.1108586215946189No Hit
GAATACCGGGTGCTGTAGGCTTT67240.1098781502951382No Hit
ATCCCGGACGAGCCCCCA65920.10772111343628064No Hit
TAGCAGCACGTAAATATTGGCGT65210.10656088906522845No Hit
TTCACAGTGGCTAAGTTCCG63060.1030475335754226No Hit
CCCATAAAGTAGAAAGCACTA63030.10299851001044856No Hit
ACTGGACTTGGAGTCAGAAGGCA63030.10299851001044856No Hit
TTCACAGTGGCTAAGTTCTG62790.10260632149065627No Hit
ACTGGACTTGGAGTCAGAAGGCT62770.10257363911400691No Hit
GTCAGGATGGCCGAGCGGTCTAAGG62690.1024429096074095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)