FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9437491
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5819926.1668085299366115No Hit
GCCTGTCTGAGCGTCGCT4074234.3170690176022415No Hit
AAGCTGCCAGTTGAAGAACTGT3701623.9222500980398283No Hit
TTCAAGTAATCCAGGATAGGCT3557163.769179753389963No Hit
TAGCAGCACGTAAATATTGGCG3090213.274397824591303No Hit
AACATTCAACGCTGTCGGTGAGT2669962.829099386690806No Hit
CCCCCCACTGCTAAATTTGACTG2101062.2262908648071824No Hit
TAGCTTATCAGACTGATGTTGAC1774531.8802984818740491No Hit
TGAGGTAGTAGGTTGTATAGTT1627551.72455793600227No Hit
AACATTCAACGCTGTCGGTGAG1466791.5542160517027248No Hit
TATTGCACTTGTCCCGGCCTGTAA1428081.5131987940438831No Hit
TAGCTTATCAGACTGATGTTGACT1403221.4868570470689721No Hit
TATTGCACTTGTCCCGGCCTGTAT1370141.4518053580130565No Hit
TGTAAACATCCTTGACTGGAAGCT1223211.2961177923242524No Hit
TGAGGTAGTAGATTGTATAGTT1181661.25209126027246No Hit
TAGCTTATCAGACTGATGTTGA1147941.2163614248744714No Hit
TATTGCACTTGTCCCGGCCTGT1038631.100536148855665No Hit
AACCGGGCGGAAACACCA969791.0275930329364023No Hit
CGCCTGTCTGAGCGTCGCT901630.9553704475055923No Hit
AGCAGCATTGTACAGGGCTATGA824520.8736644093223506No Hit
TCCTGTCTGAGCGTCGCT803310.8511902157045765No Hit
TTCAAGTAATTCAGGATAGGTT711550.7539609839098125No Hit
CCCCCACTGCTAAATTTGACTG696140.737632491517078No Hit
TATTGCACTTGTCCCGGCCTGTA681230.7218338009540883No Hit
CATAAAGTAGAAAGCACTACT660590.6999635814222234No Hit
TGTAAACATCCCCGACTGGAAGCT591090.6263211270876974No Hit
ACTGGACTTGGAGTCAGAAGGC589240.6243608603176416No Hit
TTAATGCTAATCGTGATAGGGGTT545600.5781197566175162No Hit
TGAGGTAGTAGTTTGTACAGTT428770.45432626107934826No Hit
TCGTACGACTCTTAGCGG407920.4322335247789905No Hit
CAACGGAATCCCAAAAGCAGCTGT384190.4070891299393027No Hit
TGTAAACATCCTACACTCTCAGCT373150.3953911055385377No Hit
CCCATAAAGTAGAAAGCACTAC363990.3856851360176132No Hit
CATTGCACTTGTCTCGGTCTGA335970.355995041478715No Hit
TACGCCTGTCTGAGCGTCGCT298110.3158784469304394No Hit
CTACGCCTGTCTGAGCGTCGCT286310.30337512374846237No Hit
GGAATACCGGGTGCTGTAGGCTTT274810.2911896816643322No Hit
TGAGGTAGTAGTTTGTGCTGTT266020.2818757654974188No Hit
AACATTCAACGCTGTCGGTGAGTT252320.2673591953624115No Hit
CCCATAAAGTAGAAAGCACT252220.2672532349964625No Hit
CCCCCACTGCTAAATTTGACTGGCTT250050.2649538950553701No Hit
TTCAAGTAATCCAGGATAGGCTT236460.2505538813229067No Hit
ATCCCGGACGAGCCCCCA234110.24806381272310618No Hit
ACGCCTGTCTGAGCGTCGCT232070.2459022212577474No Hit
TTATAAAGCAATGAGACTGATT229160.24281877460863274No Hit
GCGTTCGCGCTTTCCCCTG223120.23641876850531568No Hit
AGCTCGGTCTGAGGCCCCTCAGT219000.2320532014282186No Hit
TTCTCACTACTGCACTTGACTAG213850.22659624258184724No Hit
TAGCTTATCAGACTGATGTTGACA213290.22600286453253307No Hit
CTCCCACTGCTTCACTTGACTAG208710.22114987977207076No Hit
GGAATACCGGGTGCTGTAGGCTT203960.21611676238949526No Hit
TAGCAGCACGTAAATATTGGC196800.20853000018754986No Hit
CAAAGAATTCTCCTTTTGGGCT193700.20524522884313215No Hit
TGATGTAGTAGGTTGTATAGTT190510.20186509316936035No Hit
AGCAGCATTGTACAGGGCTATGT188560.1997988660333557No Hit
TGTCAGTTTGTCAAATACCCCAA186580.1977008507875663No Hit
TATTGCACTTGTCCCGGCCTGTT180580.19134322883062882No Hit
ACTGGACTTGGAGTCAGAAGGCA177950.1885564712061712No Hit
CAAAGAATTCTCCTTTTGGGCTT175010.18544123644727184No Hit
TTCACAGTGGCTAAGTTCCG173720.18407434772653028No Hit
TGTAAACATCCCCGACTGGAAGC171220.18142533857780632No Hit
CTTCTCACTACTGCACTTGACTAG168430.1784690443678304No Hit
TGTCAGTTTGTCAAATACCCCA165270.1751206968038433No Hit
CAACGGAATCCCAAAAGCAGCT163980.17375380808310176No Hit
TTCAAGTAATCCAGGATAGGC161180.1707869178365309No Hit
CCCCCCACTGCTAAATTTGACTGGCT160940.17053261295825342No Hit
TGTAAACATCCTACACTCAGCT160680.1702571160067861No Hit
TATTGCACTTGTCCCGGCCTGTAG160450.17001340716510352No Hit
TGAGGTAGTAGGTTGTATAGT160440.17000281112850862No Hit
TAGCAGCACGTAAATATTGGCGT158870.16833923338311No Hit
CAACACCAGTCGATGGGCTGT156680.1660187013688278No Hit
CACTAGATTGTGAGCTCCTGGA152360.1614412135598328No Hit
TCGTTTCCCGGCCAATGCACCA151850.1609008156934931No Hit
AACGGAATCCCAAAAGCAGCTG144330.15293259617413144No Hit
CACCCGGCTGTGTGCACATGTGC141200.14961603671992907No Hit
CACTAGATTGTGAGCTCCTGGAA140220.14857762513362927No Hit
TTCACAGTGGCTAAGTTCTGC138180.1464160336682705No Hit
TGATGTAGTAGATTGTATAGTT137460.14565311903343803No Hit
TGAGGTAGTAGGTTGTGTGGTT136550.1446888797033025No Hit
TCGTTTCCCGGGCGGCGCACCA129830.13756834311153252No Hit
ACCACAGGGTAGAACCACGGAC129220.13692198487924384No Hit
ATCCCACCGCTGCCACCA128900.1365829117082072No Hit
AGCAGCATTGTACAGGGCTATG127590.13519483091427584No Hit
ACTGGACTTGGAGTCAGAAGGCT126810.13436834005987394No Hit
CAAAGTGCTGTTCGTGCAGGTAG125320.1327895306072345No Hit
TTCACAGTGGCTAAGTTCCGC124950.13239747725322334No Hit
TGAGAACTGAATTCCATGGGTT123580.1309458202397226No Hit
ACTGGACTTGGAGTCAGAAGG122360.12965310377514533No Hit
AGAGGTAGTAGGTTGCATAGTT120250.12741734005362232No Hit
TATTGCACTTGTCCCGGCCTGTTT118830.1259127028571471No Hit
CAAAGTGCTGTTCGTGCAGGT115870.12277627602505793No Hit
CAGGTCCCTGTTCGGGCGCCA115000.12185442084130199No Hit
CCAAGTCCTTCTGATCGAGGCCC114460.12128223486517761No Hit
ACCGGGTGCTGTAGGCTT112530.11923719980236273No Hit
CCCCCCACTGCTAAATTTGACTGG112320.1190146830338699No Hit
TGAGGTAGTAAGTTGTATTGTT110540.11712858851997845No Hit
TGAGGTAGTAGATTGTATAGT109570.11610077297027356No Hit
ACCGGGTGCTGTAGGCTTA104440.11066500619709199No Hit
CTCCCACTGCTTCACTTGACTAGCCT103430.10959480650100753No Hit
ATCACATTGCCAGGGATTTCCA102520.108630567170872No Hit
TGTAAACATCCTTGACTGGAAGC101170.10720010223056106No Hit
TTCACAGTGGCTAAGTTCTG100760.10676566473017032No Hit
TATTGCACTTGTCCCGGCCTGTAAT100160.10612990253447659No Hit
CCTCGACACAAGGGTTTGT99200.10511268302136659No Hit
ATCACATTGCCAGGGATTTCC96270.10200804429906213No Hit
TGAGGTAGTAGGTTGTATAGTTA94650.10029148637068898No Hit
TGTCAGTTTGTCAAATACCCC94450.10007956563879107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)