FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8258192
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT120945414.645505941252033No Hit
CAACGGAATCCCAAAAGCAGCTG90202210.922754036234565No Hit
TTCAAGTAATCCAGGATAGGCT2766573.350091642335271No Hit
TAGCAGCACGTAAATATTGGCG2448542.9649831343228636No Hit
CCCCCCACTGCTAAATTTGACTG2123792.5717372519311734No Hit
AACATTCAACGCTGTCGGTGAGT2120302.5675111452966948No Hit
AAGCTGCCAGTTGAAGAACTGT1899592.300249255527118No Hit
TCCTGTCTGAGCGTCGCT1595311.931790881103273No Hit
AACATTCAACGCTGTCGGTGAG1461481.769733617237284No Hit
TGAGGTAGTAGGTTGTATAGTT1311831.5885196178533993No Hit
TATTGCACTTGTCCCGGCCTGTAA918511.1122410329040546No Hit
CCCCCACTGCTAAATTTGACTG885601.0723896949840837No Hit
AACCGGGCGGAAACACCA854501.0347301201037709No Hit
TAGCTTATCAGACTGATGTTGACT837611.0142777014629836No Hit
TAGCTTATCAGACTGATGTTGAC807430.9777321718845966No Hit
TATTGCACTTGTCCCGGCCTGTAT752020.9106351608197049No Hit
TAGCTTATCAGACTGATGTTGA731760.8861019458012117No Hit
TGAGGTAGTAGATTGTATAGTT697360.8444463388596439No Hit
CAACGGAATCCCAAAAGCAGCTGT580500.7029383671389573No Hit
CATAAAGTAGAAAGCACTACT501050.6067308679672258No Hit
TATTGCACTTGTCCCGGCCTGT500400.6059437707430391No Hit
TTCAAGTAATTCAGGATAGGTT473480.5733458364736495No Hit
TGTAAACATCCTTGACTGGAAGCT447500.5418861658823142No Hit
TCGTACGACTCTTAGCGG429630.5202470468112149No Hit
TTAATGCTAATCGTGATAGGGGTT429390.519956426297669No Hit
CCCATAAAGTAGAAAGCACTAC406830.49263809802436176No Hit
AGCAGCATTGTACAGGGCTATGA405160.49061586361760545No Hit
ACTGGACTTGGAGTCAGAAGGC376280.45564452848759146No Hit
CCCCCACTGCTAAATTTGACTGGCTT341020.4129475313724844No Hit
TATTGCACTTGTCCCGGCCTGTA310580.3760871629044227No Hit
CGCCTGTCTGAGCGTCGCT300130.363433061377115No Hit
TGTAAACATCCCCGACTGGAAGCT285110.3452450609043723No Hit
CATTGCACTTGTCTCGGTCTGA281400.34075255213247646No Hit
CAACGGAATCCCAAAAGCAGCT275390.3334749301057665No Hit
CCCATAAAGTAGAAAGCACT264990.32088137451878085No Hit
TGAGAACTGAATTCCATGGGTT261960.31721229053526484No Hit
TGAGGTAGTAGTTTGTACAGTT227390.27535082739660205No Hit
TGTAAACATCCTACACTCTCAGCT219900.2662810455363595No Hit
AACGGAATCCCAAAAGCAGCTG209090.2531910132387331No Hit
CCCTGTCTGAGCGTCGCT198790.2407185495323916No Hit
CAAAGAATTCTCCTTTTGGGCT194780.23586276511856347No Hit
TAGCAGCACGTAAATATTGGC186910.2263328341118734No Hit
TGAGAACTGAATTCCATAGGCTGT179900.2178442932787225No Hit
CAACACCAGTCGATGGGCTGT174310.21107525715071776No Hit
CTACGCCTGTCTGAGCGTCGCT172000.20827803470783918No Hit
CACCCGGCTGTGTGCACATGTGC166510.2016300904604785No Hit
AACATTCAACGCTGTCGGTGAGTT152790.185016284436109No Hit
TGAGGTAGTAGTTTGTGCTGTT150230.18191633229162024No Hit
AGCTCGGTCTGAGGCCCCTCAGT150080.1817346944706541No Hit
TGATGTAGTAGGTTGTATAGTT147480.1785863055739077No Hit
TTCTCACTACTGCACTTGACTAG146710.1776538980929482No Hit
CACTAGATTGTGAGCTCCTGGA146370.17724218569875827No Hit
CTCCCACTGCTTCACTTGACTAG142220.17221687265202842No Hit
CCCCCCACTGCTAAATTTGACTGGCT133220.1613186033940601No Hit
CAAAGAATTCTCCTTTTGGGCTT128420.15550619312314365No Hit
CACTAGATTGTGAGCTCCTGGAA127810.1547675326512147No Hit
TTCAAGTAATCCAGGATAGGC125480.15194609183220734No Hit
TCCGGTCTGAGCGTCGCT125260.15167968969479032No Hit
TTCACAGTGGCTAAGTTCCGC124470.150723063837702No Hit
TCGTTTCCCGGCCAATGCACCA122480.14831333541288455No Hit
TTCAAGTAATCCAGGATAGGCTT121550.14718718092289448No Hit
CACCCGTAGAACCGACCTTGCG116950.14161695441326624No Hit
ACTGGACTTGGAGTCAGAAGGCA114860.1390861341078047No Hit
TTCACAGTGGCTAAGTTCTGC113360.13726975589814333No Hit
ATCACATTGCCAGGGATTTCC108950.13192960396173883No Hit
TGAGGTAGTAGGTTGTATAGT108900.1318690580214168No Hit
CTTCTCACTACTGCACTTGACTAG108530.13142101806303366No Hit
TGAGGTAGTAGGTTGTGTGGTT108070.13086399541207083No Hit
ACTGGACTTGGAGTCAGAAGGCT107390.130040570623691No Hit
GCCGGTCTGAGCGTCGCT105500.12775193407951765No Hit
CCCATAAAGTAGAAAGCACTA104190.12616563044308005No Hit
GGAATACCGGGTGCTGTAGGCTTT104100.12605664775050035No Hit
TACGCCTGTCTGAGCGTCGCT101300.12266607509246576No Hit
CCCCCCACTGCTAAATTTGACTGG100570.12178210436376388No Hit
TAGCTTATCAGACTGATGTTGACA100010.12110398983215696No Hit
ATCCCGGACGAGCCCCCA99880.12094657038731965No Hit
TGAGGTAGTAAGTTGTATTGTT96200.11649038917961704No Hit
GCCTGTCTGAGCGTCGCTT95840.1160544584092983No Hit
TATTGCACTTGTCCCGGCCTGTAG94090.1139353504980267No Hit
AGAGGTAGTAGGTTGCATAGTT91260.11050845027579888No Hit
TATTGCACTTGTCCCGGCCTGTAAT87360.10578586693067926No Hit
ACGCCTGTCTGAGCGTCGCT87060.10542259128874698No Hit
TATTGCACTTGTCCCGGCCTGTT85160.10312184555650922No Hit
TTCACAGTGGCTAAGTTCCG84120.10186248999781067No Hit
AGCAGCATTGTACAGGGCTATGT83800.10147499597974957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)