FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7840989
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6161707.858319913470099No Hit
GCCTGTCTGAGCGTCGCT5511367.028909235811962No Hit
TTCAAGTAATCCAGGATAGGCT3138894.003181231347219No Hit
AAGCTGCCAGTTGAAGAACTGT2643513.371398684528189No Hit
TAGCAGCACGTAAATATTGGCG2599213.3149007095916088No Hit
AACATTCAACGCTGTCGGTGAGT2336332.9796368799905215No Hit
TCCTGTCTGAGCGTCGCT2188842.7915356085820298No Hit
CCCCCCACTGCTAAATTTGACTG1711432.1826710890679735No Hit
AACATTCAACGCTGTCGGTGAG1685442.149524760205632No Hit
TGAGGTAGTAGGTTGTATAGTT1635322.0856042522186935No Hit
AACCGGGCGGAAACACCA1196701.5262105328804823No Hit
TAGCTTATCAGACTGATGTTGAC1135361.4479806055078002No Hit
TAGCTTATCAGACTGATGTTGA1077291.3739210704159897No Hit
TGAGGTAGTAGATTGTATAGTT1037521.3232004279052043No Hit
TAGCTTATCAGACTGATGTTGACT975261.243797179156864No Hit
TATTGCACTTGTCCCGGCCTGTAA961351.2260570700966422No Hit
TTAATGCTAATCGTGATAGGGGTT838471.0693421454870042No Hit
TATTGCACTTGTCCCGGCCTGTAT828621.0567799546715344No Hit
TATTGCACTTGTCCCGGCCTGT723650.9229065364075884No Hit
CCCCCACTGCTAAATTTGACTG718980.9169506550768023No Hit
CATAAAGTAGAAAGCACTACT666740.8503264065285642No Hit
TGTAAACATCCTTGACTGGAAGCT648920.8275996816217953No Hit
TTCAAGTAATTCAGGATAGGTT631690.8056254128146334No Hit
AGCAGCATTGTACAGGGCTATGA534200.6812916074745162No Hit
TATTGCACTTGTCCCGGCCTGTA518960.6618552838168756No Hit
ACTGGACTTGGAGTCAGAAGGC410940.5240920501227587No Hit
CGCCTGTCTGAGCGTCGCT387070.49364946182171665No Hit
CAACGGAATCCCAAAAGCAGCTGT362150.46186775673323865No Hit
TGAGGTAGTAGTTTGTACAGTT350150.44656356487682863No Hit
TGTAAACATCCCCGACTGGAAGCT333800.42571160347247017No Hit
CAACGGAATCCCAAAAGCAGCT308190.39304990735224854No Hit
CATTGCACTTGTCTCGGTCTGA271580.3463593686969845No Hit
CCCATAAAGTAGAAAGCACTAC258620.32983084149206177No Hit
TCGTACGACTCTTAGCGG239400.30531862753537853No Hit
CCCATAAAGTAGAAAGCACT237700.30315053368905376No Hit
TTCACAGTGGCTAAGTTCCG225670.2878080813530028No Hit
TGAGGTAGTAGTTTGTGCTGTT211940.27029753517062705No Hit
CAACACCAGTCGATGGGCTGT206830.2637805001384392No Hit
TAGCAGCACGTAAATATTGGC204130.26033705697074694No Hit
CCCTGTCTGAGCGTCGCT192260.24519866052611475No Hit
AACATTCAACGCTGTCGGTGAGTT187420.23902596981069607No Hit
AACGGAATCCCAAAAGCAGCTG183420.23392457252522608No Hit
TGAGAACTGAATTCCATGGGTT182570.23284052560206375No Hit
CCCCCACTGCTAAATTTGACTGGCTT177030.22577509036168777No Hit
TGTAAACATCCTACACTCTCAGCT176530.22513741570100404No Hit
CAAAGAATTCTCCTTTTGGGCT172590.22011253937481612No Hit
TTATAAAGCAATGAGACTGATT165770.21141465700308978No Hit
TACGCCTGTCTGAGCGTCGCT165360.2108917637813291No Hit
TTCTCACTACTGCACTTGACTAG164190.20939960507532918No Hit
TGAGGTAGTAGGTTGTATAGT163100.20800947431503855No Hit
AGCTCGGTCTGAGGCCCCTCAGT159530.2034564772377566No Hit
TTCACAGTGGCTAAGTTCTG157250.2005486807850387No Hit
TCGTTTCCCGGCCAATGCACCA156280.19931159194331227No Hit
CTACGCCTGTCTGAGCGTCGCT154970.19764088433232083No Hit
TTCAAGTAATCCAGGATAGGC149850.19111109580691926No Hit
TAGCTTATCAGACTGATGTTGACA143530.1830508880958767No Hit
TTCAAGTAATCCAGGATAGGCTT142110.18123989205953484No Hit
TTCACAGTGGCTAAGTTCTGC140900.17969671938068016No Hit
TGAGGTAGTAGGTTGTGTGGTT136630.17425097777844095No Hit
TATTGCACTTGTCCCGGCCTGTT136280.17380460551596233No Hit
ACCGGGTGCTGTAGGCTT122090.15570739864575758No Hit
TGAGGTAGTAAGTTGTATTGTT120040.1530929325369542No Hit
AGCTACATTGTCTGCTGGGTTT112070.14292839844565527No Hit
ACTGGACTTGGAGTCAGAAGGCA109380.1394977087711767No Hit
CACTAGATTGTGAGCTCCTGGA108260.1380693175312451No Hit
CTCCCACTGCTTCACTTGACTAG107950.13767395924162118No Hit
ATCCCGGACGAGCCCCCA104940.13383515778430502No Hit
ACCGGGTGCTGTAGGCTTT104680.13350356696074947No Hit
CACCCGGCTGTGTGCACATGTGC104620.13342704600146743No Hit
AGCAGCATTGTACAGGGCTATGT104010.13264908291543326No Hit
ACGCCTGTCTGAGCGTCGCT104000.1326363294222196No Hit
TATTGCACTTGTCCCGGCCTGTAG103070.1314502545533478No Hit
TGAGGTAGTAGATTGTATAGT102910.131246198661929No Hit
AGAGGTAGTAGGTTGCATAGTT102860.13118243119586062No Hit
CAACGGAATCCCAAAAGCAGCTGA102590.1308380868790914No Hit
CAAAGAATTCTCCTTTTGGGCTT101960.13003461680662987No Hit
ACTGGACTTGGAGTCAGAAGG99870.1273691367249718No Hit
ACCGGGTGCTGTAGGCTTA99090.12637436425430518No Hit
TAGCAGCACGTAAATATTGGCGT97070.12379815862514282No Hit
GGAATACCGGGTGCTGTAGGCTTT96320.1228416466341172No Hit
TTAGGTAGTAGGTTGTATAGTT95840.12222947895986082No Hit
ACTGGACTTGGAGTCAGAAGGCT95710.12206368354808303No Hit
CTTCTCACTACTGCACTTGACTAG95660.12199991608201466No Hit
TGTAAACATCCCCGACTGGAAGC94210.1201506595660318No Hit
AGCAGCATTGTACAGGGCTATG93690.1194874779189207No Hit
CACTAGATTGTGAGCTCCTGGAA91680.11692402578297202No Hit
CACCCGTAGAACCGACCTTGCG91220.11633736509514297No Hit
TTAATGCTAATCGTGATAGGGGT90250.11510027625341651No Hit
AATGACACGATCACTCCCGTTG90080.11488346686878402No Hit
TGTCAGTTTGTCAAATACCCCAA89410.1140289828234678No Hit
CAAAGTGCTGTTCGTGCAGGT88820.11327652672386099No Hit
TTCACAGTGGCTAAGTTCCGC87080.11105741890468153No Hit
TGAGAACTGAATTCCATAGGCTGT86040.10973105561045933No Hit
ACCACAGGGTAGAACCACGGAC85550.10910613444298928No Hit
TGTCAGTTTGTCAAATACCCCA84420.107664989709844No Hit
TTAATGCTAATCGTGATAGGGGTTT81110.10344358345611758No Hit
GCCTGTCTGAGCGTCGCA79150.10094389878623729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)