FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979388
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT112112512.485539103555833No Hit
CAACGGAATCCCAAAAGCAGCTG7534808.391217753370274No Hit
CCCCCCACTGCTAAATTTGACTG3900544.343881787934769No Hit
TTCAAGTAATCCAGGATAGGCT3366613.749264426484299No Hit
AAGCTGCCAGTTGAAGAACTGT3039763.3852641182227563No Hit
TAGCAGCACGTAAATATTGGCG2838743.16139585459499No Hit
AACATTCAACGCTGTCGGTGAGT1811752.017676483074348No Hit
AACATTCAACGCTGTCGGTGAG1582191.7620243161337943No Hit
TGAGGTAGTAGGTTGTATAGTT1403261.562756838216591No Hit
CCCCCACTGCTAAATTTGACTG1335861.4876960434274584No Hit
TATTGCACTTGTCCCGGCCTGTAT1008361.1229718550974743No Hit
TATTGCACTTGTCCCGGCCTGTAA1007321.1218136469879685No Hit
TAGCTTATCAGACTGATGTTGAC948041.055795784746132No Hit
TATTGCACTTGTCCCGGCCTGT903581.0062823880647545No Hit
TGAGGTAGTAGATTGTATAGTT885270.985891243367588No Hit
TAGCTTATCAGACTGATGTTGACT784470.8736341496770158No Hit
CATAAAGTAGAAAGCACTACT730140.81312891257177No Hit
TAGCTTATCAGACTGATGTTGA675300.7520557080282086No Hit
TTCAAGTAATTCAGGATAGGTT657170.7318650224269182No Hit
TGTAAACATCCTTGACTGGAAGCT655070.7295263329750312No Hit
TCGTACGACTCTTAGCGG633050.7050035035795312No Hit
AACCGGGCGGAAACACCA551650.6143514457778191No Hit
CAACGGAATCCCAAAAGCAGCTGT535490.5963546736147274No Hit
TCCTGTCTGAGCGTCGCT525630.5853739698072964No Hit
AGCAGCATTGTACAGGGCTATGA461450.5138991655110571No Hit
TATTGCACTTGTCCCGGCCTGTA452440.5038650741008185No Hit
CCCATAAAGTAGAAAGCACTAC443950.49441008674533277No Hit
GGAATACCGGGTGCTGTAGGCTTT378230.42122024351770965No Hit
ACTGGACTTGGAGTCAGAAGGC373780.4162644492029969No Hit
TGAGGTAGTAGTTTGTACAGTT350940.39082841725961726No Hit
CTCCCACTGCTTCACTTGACTAG333570.3714841145075812No Hit
TTCTCACTACTGCACTTGACTAG327290.3644903193847955No Hit
CATTGCACTTGTCTCGGTCTGA326130.3631984718780389No Hit
TGTAAACATCCCCGACTGGAAGCT325820.36285323676847464No Hit
CGCCTGTCTGAGCGTCGCT313690.3493445210297183No Hit
GGAATACCGGGTGCTGTAGGCTT273940.30507647069043015No Hit
CCCCCACTGCTAAATTTGACTGGCTT254720.283671893897446No Hit
CTACGCCTGTCTGAGCGTCGCT230080.2562312709953061No Hit
TGAGGTAGTAGTTTGTGCTGTT222120.24736652431101097No Hit
CTTCTCACTACTGCACTTGACTAG221400.24656468792750688No Hit
TTCACAGTGGCTAAGTTCCGC220360.24540647981800096No Hit
CCCATAAAGTAGAAAGCACT217590.24232163706479773No Hit
TAGCAGCACGTAAATATTGGC215380.2398604448320977No Hit
CAACGGAATCCCAAAAGCAGCT205960.22936975214791921No Hit
AACGGAATCCCAAAAGCAGCTG198510.22107297290193942No Hit
ATCCCGGACGAGCCCCCA196750.21911292840892943No Hit
TTCAAGTAATCCAGGATAGGC188560.20999203954657045No Hit
TGTAAACATCCTACACTCTCAGCT188170.2095577115055057No Hit
CAACACCAGTCGATGGGCTGT183520.20437918486204185No Hit
TGAGGTAGTAGGTTGTATAGT179620.20003590445139469No Hit
AGCTCGGTCTGAGGCCCCTCAGT167770.1868390139728899No Hit
CCCCCCACTGCTAAATTTGACTGGCT167080.1860705874386985No Hit
CAAAGAATTCTCCTTTTGGGCT159950.17813017991872052No Hit
TGATGTAGTAGGTTGTATAGTT154440.17199390426162675No Hit
CCCCCCACTGCTAAATTTGACTGG143200.15947634738581293No Hit
TTCACAGTGGCTAAGTTCTGC141790.15790608446811744No Hit
TGAGGTAGTAGGTTGTGTGGTT141200.15724902409830158No Hit
ATCCCACCGCTGCCACCA140980.1570040185366753No Hit
TATTGCACTTGTCCCGGCCTGTT139610.15547830208473007No Hit
TTCAAGTAATCCAGGATAGGCTT139090.1548991980299771No Hit
CACCCGGCTGTGTGCACATGTGC134400.14967612492076296No Hit
TCGTTTCCCGGCCAATGCACCA133950.14917497718107292No Hit
ACCCCCCACTGCTAAATTTGACTG133500.14867382944138285No Hit
TACGCCTGTCTGAGCGTCGCT126650.14104524718165648No Hit
CTCCCACTGCTTCACTTGACTAGCCT126560.14094501763371847No Hit
TGTCAGTTTGTCAAATACCCCAA119840.13346121138768033No Hit
TAGCTTATCAGACTGATGTTGACA119030.13255914545623823No Hit
ACCGGGTGCTGTAGGCTT115020.12809336226477797No Hit
AGAGGTAGTAGGTTGCATAGTT113820.12675696829227115No Hit
TTCACAGTGGCTAAGTTCCG113250.12612218115533042No Hit
AACATTCAACGCTGTCGGTGAGTT111710.12440714222394667No Hit
TTATAAAGCAATGAGACTGATT111010.12362757907331769No Hit
CACTAGATTGTGAGCTCCTGGA110790.12338257351169143No Hit
TGAGAACTGAATTCCATAGGCTGT110270.12280346945693849No Hit
CAAAGAATTCTCCTTTTGGGCTT109950.12244709773093668No Hit
TGTCAGTTTGTCAAATACCCCA108890.12126661638855567No Hit
TATTGCACTTGTCCCGGCCTGTAG108540.12087683481324116No Hit
CGCGACCTCAGATCAGACGTGG106520.11862723829285471No Hit
TGAGGTAGTAGATTGTATAGT105190.11714606830665966No Hit
GCCTGTCTGAGCGTCGCG104420.11628854884096777No Hit
ACGCCTGTCTGAGCGTCGCT103410.11516375058077456No Hit
TAGCAGCACGTAAATATTGGCGT102750.1144287338958958No Hit
ATCACATTGCCAGGGATTTCC102240.11386076645758042No Hit
ACCGGGTGCTGTAGGCTTT101900.11348212149870347No Hit
GCCTGTCTGAGCGTCGCA101460.11299211037545098No Hit
TGTAAACATCCCCGACTGGAAGC100750.11220141060838446No Hit
TCCCACTGCTTCACTTGACTAG98310.1094840761976206No Hit
TGATGTAGTAGATTGTATAGTT97610.10870451304699161No Hit
ACTGGACTTGGAGTCAGAAGGCA93570.1042053200062187No Hit
CAAAGTGCTGTTCGTGCAGGTAG93150.1037375821158413No Hit
ACCGGGTGCTGTAGGCTTA90640.10094229139001455No Hit
TGTAAACATCCCCGACTGGA89950.10017386485582312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)