FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755782
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT100134414.822029485261663No Hit
CAACGGAATCCCAAAAGCAGCTG3337284.939887047865073No Hit
CCCCCCACTGCTAAATTTGACTG2273953.3659315827538547No Hit
AAGCTGCCAGTTGAAGAACTGT2225033.2935195363023855No Hit
AACATTCAACGCTGTCGGTGAGT1953432.8914935384238274No Hit
TTCAAGTAATCCAGGATAGGCT1838082.720750906408762No Hit
TAGCAGCACGTAAATATTGGCG1377692.0392753940254438No Hit
TATTGCACTTGTCCCGGCCTGT1006141.4893020526713268No Hit
TCGTACGACTCTTAGCGG978611.448551773873106No Hit
AACATTCAACGCTGTCGGTGAG947761.402887186117018No Hit
TAGCTTATCAGACTGATGTTGAC893121.322008318208018No Hit
CCCCCACTGCTAAATTTGACTG813501.2041537160316897No Hit
TATTGCACTTGTCCCGGCCTGTAT770881.1410670148918363No Hit
CGCCTGTCTGAGCGTCGCT757281.120936110727078No Hit
TGAGGTAGTAGGTTGTATAGTT739401.0944698926045868No Hit
TATTGCACTTGTCCCGGCCTGTAA701281.038044152401602No Hit
TAGCTTATCAGACTGATGTTGA625850.9263916449642692No Hit
TAGCTTATCAGACTGATGTTGACT622760.9218177851209528No Hit
AACCGGGCGGAAACACCA507060.7505570783663534No Hit
CATAAAGTAGAAAGCACTACT498580.7380048675342099No Hit
TGAGGTAGTAGATTGTATAGTT466500.6905196171220445No Hit
TATTGCACTTGTCCCGGCCTGTA428660.6345083367106872No Hit
AGCAGCATTGTACAGGGCTATGA401270.5939652878082804No Hit
ATCCCGGACGAGCCCCCA388700.5753590035912941No Hit
ACTGGACTTGGAGTCAGAAGGC378920.5608825151551663No Hit
TGTAAACATCCTTGACTGGAAGCT345380.5112361529723725No Hit
TTCAAGTAATTCAGGATAGGTT342460.5069139294311155No Hit
CATTGCACTTGTCTCGGTCTGA299360.44311672579132955No Hit
TCCTGTCTGAGCGTCGCT267530.3960015287645457No Hit
CCCATAAAGTAGAAAGCACTAC254470.3766699399122115No Hit
GCGTTCGCGCTTTCCCCTG249700.3696093213191308No Hit
CTACGCCTGTCTGAGCGTCGCT225330.3335365173121335No Hit
CCCATAAAGTAGAAAGCACT218510.3234414609589238No Hit
TACGCCTGTCTGAGCGTCGCT211380.31288753840784084No Hit
ACCGGGTGCTGTAGGCTT206880.30622657747097226No Hit
CAACGGAATCCCAAAAGCAGCTGT204820.303177337575428No Hit
TGTAAACATCCCCGACTGGAAGCT196380.2906843352849455No Hit
ACGCCTGTCTGAGCGTCGCT180390.26701572075593916No Hit
CTCCCACTGCTTCACTTGACTAG176750.26162774346478324No Hit
TGAGGTAGTAGTTTGTGCTGTT168510.24943078388260606No Hit
TGTAAACATCCTACACTCTCAGCT162620.2407123261230158No Hit
ACCGGGTGCTGTAGGCTTT162520.2405643047688632No Hit
TGAGGTAGTAGTTTGTACAGTT158120.23405136518614722No Hit
ATCCCACCGCTGCCACCA153670.2274644149263549No Hit
AACATTCAACGCTGTCGGTGAGTT153580.2273311957076176No Hit
CAACACCAGTCGATGGGCTGT142510.21094523180292085No Hit
TTCAAGTAATCCAGGATAGGCTT138820.20548324383468855No Hit
GGAATACCGGGTGCTGTAGGCTTT136500.20204914841834742No Hit
TATTGCACTTGTCCCGGCCTGTT135120.2000064537310411No Hit
ACCGGGTGCTGTAGGCTTA133040.19692760956466623No Hit
CACTAGATTGTGAGCTCCTGGA132090.1955214067002162No Hit
AGCTCGGTCTGAGGCCCCTCAGT127420.18860880946128813No Hit
TTATAAAGCAATGAGACTGATT125930.18640329128441385No Hit
TTCTCACTACTGCACTTGACTAG125760.1861516549823544No Hit
TTAATGCTAATCGTGATAGGGGTT123350.18258434034727586No Hit
TACCTGGTTGATCCTGCC122620.18150378446196164No Hit
CGCGACCTCAGATCAGACGTGG121680.18011238373292685No Hit
CTTCTCACTACTGCACTTGACTAG112340.1662871892550707No Hit
GGAATACCGGGTGCTGTAGGCTT110240.16317874081786535No Hit
ACCACAGGGTAGAACCACGGAC109350.1618613507659069No Hit
CAACGGAATCCCAAAAGCAGCT104390.1545194915999362No Hit
CTCGACACAAGGGTTTGT104240.15429745956870722No Hit
TTCACAGTGGCTAAGTTCCG103790.15363136347502038No Hit
AACGGAATCCCAAAAGCAGCTG101020.14953117196499235No Hit
TTCAAGTAATCCAGGATAGGC101000.14950156769416184No Hit
TGAGGTAGTAGGTTGTATAGT98180.14532736550705752No Hit
GCCTGTCTGAGCGTCGCTT97100.14372873488220905No Hit
AGCAGCATTGTACAGGGCTATGT95750.14173044660114847No Hit
CCTCGACACAAGGGTTTGT91700.13573558175796674No Hit
ACTGGACTTGGAGTCAGAAGG85290.1262474129567828No Hit
AGCAGCATTGTACAGGGCTATG84930.1257145360818333No Hit
TATTGCACTTGTCCCGGCCTGA84410.12494482504023961No Hit
CCCCCCACTGCTAAATTTGACTGGCT83600.12374585207160327No Hit
TTCACAGTGGCTAAGTTCCGC82600.12226563853007691No Hit
GGGCTACGCCTGTCTGAGCGTCGCT81450.12056339295732159No Hit
TAGCAGCACGTAAATATTGGCGT81240.12025254811360106No Hit
TATTGCACTTGTCCCGGCCTGTAG80740.1195124413428379No Hit
CGCGACCTCAGATCAGACGTGGCG80350.11893515806164259No Hit
CCCCCACTGCTAAATTTGACTGGCTT79970.11837267691586259No Hit
TGAGGTAGTAGGTTGTGTGGTT77850.11523462420782672No Hit
TCGTTTCCCGGCCAATGCACCA75010.11103081774989187No Hit
CACTAGATTGTGAGCTCCTGGAA74020.10956540634378079No Hit
CAAAGTGCTGTTCGTGCAGGTAG73880.1093581764479671No Hit
CACCCGGCTGTGTGCACATGTGC73570.10889931025009393No Hit
ACTGGACTTGGAGTCAGAAGGCA73480.10876609103135655No Hit
TGTCAGTTTGTCAAATACCCCAA73050.10812959920850021No Hit
TAGCAGCACGTAAATATTGGC72420.10719706467733861No Hit
TAGCTTATCAGACTGATGTTGACA72350.10709344972943177No Hit
TTCACAGTGGCTAAGTTCTGC71850.10635334295866858No Hit
CAAAGTGCTGTTCGTGCAGGT69730.10321529025063272No Hit
CCCCCCACTGCTAAATTTGACTGG69580.10299325821940376No Hit
AACATTCAACGCTGTCGGTGA69240.1024899856152848No Hit
TGTAAACATCCTACACTCAGCT69230.10247518347986954No Hit
TGATGTAGTAGGTTGTATAGTT67670.10016605035508842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)